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  1. The Addgene Antibody Data Hub

    Type
    Blog Post
    Published
    Sept. 12, 2024, 1:15 p.m.
    ... your research, you've probably found yourself staring at a browser full of tabs, each open to a different... a report with a DOI for citation. If you’re considering requesting an Addgene antibody, the Data Hub ...

  2. 500,000 Plasmids Shipped and Counting!

    Type
    Blog Post
    Published
    March 26, 2015, 3:24 p.m.
    ...extremely proud to be able to support scientists by bringing you all the plasmids you need. We're focused on... on our mission – documenting your plasmids, storing them, providing quality control and customer support...

  3. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ...infected in the United States while caring for a patient, despite wearing protective gear, and now comes news...

  5. What's New in CRISPR - May 2019

    Type
    Blog Post
    Published
    May 28, 2019, 12:49 p.m.
    .... coli genome-wide CRISPR inhibition Covalent tethering of DNA template to Cas9 SECURE base editors Nme2Cas9...genome-wide CRISPR inhibition library Covalent tethering of DNA repair template to Cas9 Homology-directed...

  6. A Conference By Postdocs For Postdocs: Future of Research

    Type
    Blog Post
    Published
    Sept. 2, 2014, 3:05 p.m.
    ...few of these trainees are sufficiently prepared during their 6-12+ (!) years of training to get jobs. ...designing career exploration programs or peer mentoring groups. But for now, you can click here to register...

  7. 6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results

    Type
    Blog Post
    Published
    June 26, 2014, 2:23 p.m.
    ...published on BitesizeBio here. As part of my job ensuring plasmid quality at Addgene, I analyze 50-100 sequencing... difficult template. Anything more and you’re venturing into the uncertain terrain. 4. Never trust the...

  8. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    Published
    Dec. 29, 2020, 2:15 p.m.
    ... service. We've had 11 blog posts on COVID-19 featuring plasmid tools from depositors who quickly shared...19 pandemic, we saw the need to expand reagent sharing even broader and began working with tech transfer...

  10. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    Published
    Jan. 11, 2017, 8:47 p.m.
    ...2016 was an exciting year for genome engineering research. A variety of new tools came out including ...here. References 1.Ran, F. Ann, et al. "Genome engineering using the CRISPR-Cas9 system." Nature protocols...

  11. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    Published
    Aug. 28, 2018, 12:04 p.m.
    ...ultrasound. In his research he applies naturally occurring bacterial “molecular buoyancy devices” (also known... Post on CRISPR Methods for Bacterial Genome Engineering Learn about Science Communication using Twitter...

  13. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    Published
    Jan. 23, 2014, 3:23 p.m.
    .... “What is different is the plasmids you use to bring this into your target organism. We have designed...,” Lohmann said. “We strongly believe the free sharing of material is absolutely essential for scientific...

  14. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ...transcription-activator-like effectors (TALEs) as anchors for genome engineering applications was still very new. Now, Konermann...flexible their LITE system could be by applying it to altering gene expression via epigenetics with the addition...

  15. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    Published
    Dec. 31, 2019, 2:26 p.m.
    ...Farewell 2019, and hello 2020! As we're gearing up to start a new decade, we’re also looking back at ...strategies for generating GFP fusion proteins. From considering protein size and shape, linker sequences, and...

  16. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    Published
    Jan. 17, 2018, 2:57 p.m.
    ...viral service in late 2016 and requests are still pouring in! While our lentiviral service is going strong...which cells are successfully infected by an AAV by altering your injection site and choosing a specific serotype...

  17. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    Published
    April 27, 2017, 2:30 p.m.
    ...broken down below for specific topics discussed during the interview. Happy listening! Listen to the interview... Sit'N Listen - In their podcast, the SITN team brings in scientists from a variety of fields as well ...

  18. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ...turns out this protein has a few more secrets - during a collaboration, members of Maureen Hanson’s and... Mary Gearing...

  20. Science as Art

    Type
    Blog Post
    Published
    March 2, 2015, 3:13 p.m.
    ...www.microworldsphotography.com/. Another artist who spent his life blurring the lines between science and art was Albert G...smaller competitions are now taking place and gathering momentum, including in Cambridge and Bristol. ...

  21. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    Published
    Feb. 18, 2015, 3:09 p.m.
    ...ectopic reporter assays. We have been interested in exploring the function of non-coding regulatory elements...Orkin and Daniel Bauer. He received his BSE in Bioengineering from the University of Pennsylvania.     References...

  22. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    ...the sensitivity of nLightG. Many thanks to Mike Waring and Tommaso Patriarchi for their assistance with.... (2023). Sensitive multicolor indicators for monitoring norepinephrine in vivo. Nature Methods, 20(9)...

  23. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    .... Read the paper in Cellular and Molecular Bioengineering Find the optogenetic repressor plasmids Cytosine...RNA-guided methylcytosine editing by TET1 by co-delivering TET1 and protein factors for linking methylcytosine...

  25. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    Published
    Feb. 20, 2014, 4:57 p.m.
    ...Eric Lander, comprise 73,171 guide RNAs (gRNAs) covering a total of 7,114 human genes. Unlike the GeCKO...cycle proteins, and more.  “In the past, genome engineering with CRISPRs was limited to modification of a...

  26. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    Published
    June 29, 2023, 7 p.m.
    ... lot of different worries that come along with entering the workforce, but my main concerns were building...larger content and marketing team, and I started preparing for my first official professional presentation...

  27. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ... organoids derived from other tissue-types. By bringing together CRISPR-based approaches and organoid ...the fluorescence intensity of the cpGFP without altering that of the internal control (Fig 1). Because ...

  28. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    Published
    Dec. 3, 2020, 2:15 p.m.
    ...the AAV p40 promoter. Alternative splicing and differing translational start sites generate the three VP...ratio (VP1:VP2:VP3). There are eleven naturally occurring AAV serotypes that have thus far been identified...

  29. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...gene. In cells where the stop cassette is removed during the recombination step, the effector is expressed...Learn about Gateway cloning Read about genome engineering in flies Learn more about controlling gene expression...

  30. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...reporter plasmids, viral plasmids, and genome engineering plasmids. Some of the many things that plasmids...controlled changes to an organism’s genome (genome engineering) Produce synthetic viruses that can be used in...

  33. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    Published
    Nov. 22, 2016, 3:30 p.m.
    ... online course had three aspects to it: videos starring Roberto Kolter, webinars taught by Peggy Cotter...the online class, we met as a group for sessions covering individual portions of the manuscript. Stephen...

  34. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ... science project started when I realized I was pouring large agarose gels for DNA visualization or isolation... had to be careful not to stick your fingers in during a run or risk minor electrocution.  These days ...

  35. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...infection, that is. Thankfully, through the use of engineering controls — changes made to the virus that eliminate...and Man: A History of Interactions (pp. 23–40). Springer International Publishing. https://doi.org/10.1007...

  36. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ... high G+C content genes, as validated through monitoring the expression of fluorescent proteins and oxytetracycline...-nucleotide modifications to edit DNA without requiring a double-stranded break. The Xiao Wang Lab has...

  37. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    Published
    Sept. 14, 2021, 1:15 p.m.
    ...intramolecular DNA recombination. This naturally occurring process is mediated by recombinases like RecA ...bacterial selection marker and origin of replication. During bacterial culture, only the plasmid with the antibiotic...

  39. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...transduction?  Transduction is the process of transferring foreign nucleic acid into a cell by a viral ...Expressed episomally long-term? Expressed short-term? Answering these questions will help guide you to the right...

  41. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ...plan to continue updating the feature database. Powering the plasmid maps on Addgene.org with SnapGene ... powered plasmid maps Learn how we use SnapGene during the plasmid quality control process Check out full...

  42. The Strength of Story Telling

    Type
    Blog Post
    Published
    Dec. 19, 2017, 2 p.m.
    ...talks and interviews. They even tell short stories during weekly lab meetings. When scientists tell good ...when appropriate. It might not be scientific to bring emotional discovery into a scientific story, but...

  43. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ... ITR integrity and prevent unwanted mutations?  During propagation, mutants rapidly replicate in a growth...help to reduce the likelihood of ITR deletions occurring.  How do I validate my ITR sequences?  Next-generation...

  45. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...therefore titer may not need to be as high when delivering receptor constructs with AAV. In the case of ...less particles to be released into suspension and lowering the number of viral particles that can be harvested...

  46. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...tech at Addgene, part of my job, in addition to preparing DNA and generating/replenishing kits, is to find...also gave us a few goodies which included an NEB drawstring backpack and a double wall insulated glass to...

  48. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ... a 4th year PhD student at MIT in Biological Engineering and is interested in developing synthetic biology...research Learn how the Arabidopsis research center brings plant science to life Resources on Addgene.org...

  49. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...multiplex strategies have been facing difficulties in pairing with base editing and prime editing approaches....also enable more convenient multiplex genome engineering of crops or allow for sophisticated CRISPR screenings...

  50. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ...are there. Make the most of everyone’s time by considering the level of meeting you need. Project Meetings...review the agenda and add anything that is missing.  During the meeting, take notes on the agenda points. At...
Showing: 551 - 600 of 831 results