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...presented my research to Addgenies and friends. These invited guests came to see the research from all the... interview process with Amy, who let me know in March that I successfully secured an internship at Addgene...project was based on plasmid cloning and I, the researcher, got to choose what gene to focus on. The gene...workspace — and got the chance to work on my very own research project! Coming from an environment with limited...

...encourage researchers all around the world by showing them that they are not alone; a researcher doing some... people all around the world to the lives of researchers and PhD students. Everybody in science knows ... experience this very human side to scientific research. Find Protocols Lab Tips to Keep Your Experiments...Blog Learn How You Can Use Video to Share Your Research 5 Reasons to Use Reddit for Science Communication...

...plasmids and your research stories. Consider practicing communicating your research by guest blogging ...make it a habit to talk – really talk – about the research you are doing in the lab with your mother or father...for science outreach. Here in the North Carolina Research Triangle area, scientists give talks and science...goal is ostensibly to get some funding for your research, SciFund is really motivated more by the desire...

...that will teach you how to: interpret data, solve research problems, develop general technical proficiency...professionally with other scientists. The specific research area is not as important as choosing a supportive... want to work on in your future career, so the research topic will be more important. In addition, it ...podcast interview with Harvard Medical School researcher Connie Cepko to learn about her mentoring style...use publication records, university websites, ResearchGate and LinkedIn to find past lab members. Most ...

...over and over again until you don’t.” As every researcher can attest to, science is nothing if not solving...I sit down and talk to a scientist about their research, these conflicts come out quite naturally as they...What did those odd results mean? How did the researchers solve the problem preventing them from moving...questions are next or how this will impact future research or policy.  It is worth mentioning that this is...audience a chance to get curious and invested in the research itself - and isn’t that sense of curiosity and...

...with whoever cares to listen. In March 2020 she will start her own research group at Boyce Tompson Institute...contributed by Magdalena Julkowska, a postdoctoral researcher at KAUST, Saudi Arabia. From the perspective...to the subsequent journal.  As a postdoctoral researcher studying plant development and abiotic stress...salt-stress induced responses in Root System Architecture of Arabidopsis HapMap population in Amsterdam...

...environments has never been easy and, for many years, researchers lacked good tools to directly follow immune cells...thymus to the lymph nodes. These methods gave researchers much more information regarding immune cell mobility...coloured lymphoid organs did in the past. How do researchers track fluorescently labeled cells? 1. Epifluorescence...For the first time, fluorescent proteins gave researchers the ability to track immune cells within organs... the next revolution in cell imaging, giving researchers the ability to monitor cellular movements in ...cells is the the Cre/lox recombination system. A researcher can flank fluorescent proteins in a plasmid with...

... S. cerevisiae and S. pombe have dominated the research scene. But what about the other yeasts – in the...all, but it’s not as well-known in the general research community. Phillips Petroleum Company first commercially...the K. phaffii expression system to the general research community in the 1990s when they abandoned the... and pharmaceutical companies (along with some research laboratories) have picked up the system for some...phaffi as Emerging Model Organisms in Fundamental Research. Front. Microbiol. 11-2020, 2021. DOI: 10.3389...

... deal of scientific research is funded by the public and performed by researchers who received their educations...even minor weakness or limitations in a piece of research (Drummond & Fischhoff, 2017; Kahan et al., 2012...However, much of the information gained from that research remains inaccessible, behind paywalls and in technical... Communication at the University of Otago. Her research focuses on misinformation about sexual and reproductive...

...Loren Looger’s lab at HHMI Janelia Research Campus to give researchers the ability to monitor glutamate ...iGluSnFR3 To improve upon the SF-iGluSnFR, the researchers did the following: Improve sensor’s photophysical.... In addition to the original PDGFR TMD, the researchers introduce iGluSnFR3 in two additional anchoring...Stargazin including a terminal PDZ ligand. The researchers recommend using the SGZ for in-culture work only...

...experiments and other researchers by implementing reproducibility best practices in your research. For a place ...GHP_HarvardChan — Shekhar Saxena (@ssaxenageneva) March 16, 2020 Give back to your community. For those...experiments.  Use Addgene’s AAV Data Hub. You can search the AAV Data Hub for tools to use in future experiments...contribute data to the AAV Data Hub to help the research community. Set expectations for your productivity...

...meaning, making it difficult or impossible for researchers to track down that resource. A more long-term...information, and more. The plasmid page also helps researchers find other publications that have used the same...support standardized persistent identification of research reagents, several journals or organizations now...several journals like eLife, Nature, and Cell use  Research Resource identifiers (RRIDs) which are supported...repository are verified and identifiable to save researchers time and make their work more reproducible. While...

...guest blogger Chris Richardson, a Postdoctoral Researcher in Jacob Corn’s lab. CRISPR-Cas9 (Cas9) is an...strategies to manipulate that process. We began our research with a simple question: how do Cas9 and sgRNA ...donor DNA. Chris Richardson is a Postdoctoral Researcher in Jacob Corn’s lab investigating DNA repair ...delivery of purified Cas9 ribonucleoproteins." Genome research 24.6 (2014): 1012-1019. PubMed PMID: 24696461....Our CRISPR Guide Find CRISPR Plasmids for Your Research Check out Our List of gRNA Design Tools ...

...requirements. These different PAM requirements allow researchers to target many different genomic loci. 3. Cleave.... When using CRISPR/Cas9 for genome editing, researchers simply need to express a gRNA designed to direct...elements separated by unique sequences. When researchers first discovered these arrays, they did not know...them “spacers” (see figure below). After years of research, we now know that each direct repeat, combined...leaving a F) double-strand break. A “gRNA” is a researcher-designed hybrid of the tracrRNA and the crRNA...

... Neural Dynamics and HHMI’s Janelia Research Campus. His research focuses on developing biosensors and...GECIs) have become a vital tool in neuroscience research. These fluorescent proteins light up in response...sensors in multicolor experiments. With OCaMP, researchers can image multiple brain regions or cell types...medical student at the University of Calgary and a research associate with Alexander Lohman at the Hotchkiss...

...interaction). The modularity of the system provides researchers better flexibility and makes the approach more...generate “single-use” transgenic lines. Instead, researchers can generate and maintain transgenic lines that...error rate. For a one kilobase insertion, the researchers reported an editing error rate of less than 0.3%...   This new barcoding technology would allow researchers to avoid the off-target effects of hi-multiplexing... to 200-plex with two barcodes per cell. The researchers validated their work with the “signalome,” a ...

...continue to accelerate research and discovery by improving access to useful research materials and information...have been lost to continue to be available to the research community.    Validation For most cloning projects...construct by Sanger sequence analysis. But for this research, it was likely that the sequence of the functional...range secondary antibodies are commonly used in research laboratories to detect the different subclasses...

...iPS cells and embryos make a case for conducting research in embryos should our society decide that germline...not being used to establish a pregnancy, the researchers were able to examine each individual blastomere...embryos and iPS models, it appears that embryo research is the only way to answer questions of efficiency...Given moral and ethical concerns surrounding such research, it is crucial that scientists proceed with caution...non-biologists alike. Given the public significance of this research, we have the obligation to discuss it as openly...

...experimental tools. They certainly are very handy in research, but we’re really borrowing these tools from Mother...think of bacteria when we think of plasmids, both Archaea and Eukaryota can also harbor these circular DNA...plasmids can be dangerous, they also provide researchers with new means to deliver DNA and other molecules...tend to be small and poorly studied, but some researchers have suggested they may have relevance to human...natural plasmids and their functions has enabled researchers to develop and deposit the many plasmid tools...

...discovery, researchers have done significant work to improve the Adv genome’s utility in research and its...Adenoviral vectors (AdV) are attractive vectors for research applications and gene therapy: they can be produced...PMID: 19373227. 4. Mittereder, Nanette, Keith L. March, and Bruce C. Trapnell. "Evaluation of the concentration...Resources on the Addgene Blog Using virus in your research - a primer for beginners Adenoviral delivery of...