We narrowed to 582 results for: BASE;

...number of ways, from small edits that change single base pairs, to chromosomal inversions and large deletions...1 CRISPR systems make up the majority of CRISPR-based immune systems in bacteria and archaea. Class 1 ...deletions of up to hundreds or even thousands of base pairs, stopping either at boundaries defined by ...originally suspected that Cas3 moved a couple of kilobases down the strand of DNA, Cascade caught up to it...

...would represent a major step forward for CRISPR-based RNA editing - and further experiments strongly supported...CRISPR could cut DNA, the second generation could do base editing, and with the third generation came Prime...ability to now make any small changes, including base edits, insertions, and deletions. But CRISPR’s uses...deletions and small insertions, leaving a several kilobase-sized gap in the gene editing field. The AbuGoot...now developed PASTE, which can “drag and drop” kilobases’ worth of DNA with CRISPR-driven precision into...

...label based on antibody-like sensors) changes that. Developed by the Arnold Lab, ATLAS is based on an ...synapses, and cytoskeleton components) and in a FRET-based biosensor for cAMP dynamics. The mGold2 variants...

...the most popular options, so most of this post is based on reading results from a NanoDrop. The principles...calculate a sample’s nucleic acid concentration based on the sample’s absorption at 260 nm (A260). ...use a complementary assay, such as a fluorescence based assay or running an agarose gel. In the case of ...

..., technical support is often covered by the lab based team as part of their general job duties. Bigger...etc. Technical support roles are primarily office based, and email/phone are the most popular ways to communicate... pressure which are often found in sales or lab-based roles in industry. What to look forward to in a ...answers for customers, internal resources and databases are scrutinized, and this information is enriched...

... the very beginning of the third chromosome, ten base pairs in length. An emerging family of sequencing...increase the expression of a gene that is millions of base pairs away. (For details on DNA compaction and the...parsed for spCas9 PAM sites (NGG) and then filtered based on two main criteria: no TTTTT allowed (this is ...here with permission. Quilt is a collaborative database for genomics. In this article, Quilt CTO Aneesh...is CTO at Quilt Data. Quilt is a collaborative database. Aneesh's research interests span machine learning...

...common to use many preys with a single bait in Y2H-based screens. In this way, proteins that interact with...proteome of a particular cell type, or a subset based on a cellular compartment or growth stage). A prey...protein-protein interactions in mammalian cells based on protein splicing. Anal Chem. 2001; 73:2516-2521... Including a prey-only control also provides a baseline level of reporter activation. Vary the expression...

...immunoglobulin genes, recombinant antibodies are plasmid-based. Their sequences can be optimized for specificity...Arbabi-Ghahroudi et al., 2017). Although they’re based on the immune system of llamas, synthetic nanobodies...mixture of proteins. The proteins are separated based on molecular weight by SDS-PAGE, and are subsequently...well as tools for developing and producing plasmid-based antibodies - and we’re always growing our collection...

...Thinking about using antibody-based applications in your experiment...but not sure where to start or ...

...lab at Duke University developed similar systems based on the light-inducible heterodimerizing cryptochrome...systems have been developed for use with CRISPR-based gene editing (see table below). For example, the...possibilities for optogenetic manipulation of CRISPR-based systems in animal models. Use Anti-CRISPRs to Turn...Hasegawa S, Yoshimoto K, Sato M (2017) CRISPR–Cas9-based photoactivatable transcription systems to induce..., Kawano F, Suzuki H, Sato M (2015) CRISPR-Cas9-based Photoactivatable Transcription System. Chemistry...transcription Zhou et al., 2020   NPOM-caged nucleobases Cas9, photocaged-gRNA Gene editing For more...

... Santalucía Lab has developed a cloning toolkit based on the Multisite Gateway® technology from Life Technologies...MultiSite Gateway Kit is a customizable Gateway®-based toolkit that allows scientists to generate fusion... Medical School has recently developed a CRISPR-based multicolor tool enabling the imaging of multiple...cancer-associated chromosome shattering. This method is based on the recognition of genomic loci by three orthogonal...does it work? RUSH is an ingenious two-state assay based on the reversible interaction between a hook protein...Neveu laboratory designed a new iterative, chaining-based cloning method that simplifies the process of constructing...presented Archaerhodopsin 3 (Arch) as a new rhodopsin based voltage indicator for use in the detection of neuronal...

...to provide improvements to existing technologies based on polymerase processivity. Please find the MarathonRT...Myers, a Scientist in the group, developed a CRISPR-based approach to detect proteins that associate with ...

...tools to overcome this restraint include an AAV-based sparse labeling system where a limiting amount of... truncated attP site. The three SPARC variants, based on the length of the attP site, allow for dense ...

... 2015). All of Addgene’s antibodies are plasmid-based and recombinant. Learn more about recombinant antibodies...affinity reagents, such as nanobodies and ScFvs, based on community demand. Figure 1: Antibody...from Addgene via our Antibody Data Hub once the database is available. This data will help the collection...

...Addgene’s collection also includes a base editing sensor library, a base editing knockout library, a prime...

... time, we’re developing ourselves as a knowledge base for the processes of viral production and testing...connections. Expanding the viral vector knowledge base: You can help! We’ve been expanding our viral vector...

...alternative careers. Science Careers has developed a web-based, career-planning platform called the Individual ...to learn about a variety of science professions based on their skills and interests. Once scientists identify...

...found in my tissue.” It is based on the same principles as other antibody-based assays, like an ELISA or...cases, a whole organism. But, as with any antibody-based technique, validation and proper experimental design...different companies, such as Validated Antibody Database and CiteAb. The antibody you choose can make or...

...University where she is developing fluorescent protein-based biosensors for Human Milk Oligosaccharides (HMOs...production of HMOs. Current HPLC-MS and fluorophore-based technologies are too time consuming in the first...

...should be in real scientific practice, so it is based on a win-win strategy. After each team presented...developed to teach gamma-retrovirus or lentivirus-based ex-vivo gene therapy’ strategies to undergraduates...