We narrowed to 571 results for: CHI
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TypeBlog PostPublishedJan. 15, 2019, 2:19 p.m....MerryChristmas #happyholidays2018 #DeckTheLab @Addgene @joachimgoedhart @SAbiega @EikeMahlandt @Saskia_deMan @AnoeskaMoosdijk...Fireplace from the Dyer Lab If you’re feeling as chilly this winter as the Addgenies are in Massachusetts...
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New Viral Vectors - Summer 2024
TypeBlog PostPublishedJuly 9, 2024, 1:15 p.m....AAV9 Biosensors Patriarchi New viral service pAAV_hSyn1_nLightR AAV9 Biosensors Patriarchi New viral service... -
Visualizing Your Plasmid Request Data
TypeBlog PostPublishedApril 11, 2023, 4 p.m.... your own depositor metrics! About the Author Joachim Goedhart is an assistant professor at the Section...fluorescent probes. You can follow him on twitter: @joachimgoedhart. ... -
Editor's Choice, July 2016
TypeBlog PostPublishedAug. 5, 2016, 3 p.m....was a fantastic month for the Addgene blog. We achieved roughly 50,000 views over the entire blog and ... Expression in Mammals Recombinase-based State Machines Enable Order-dependent Logic in vivo As always... -
Providing Plasmids to Researchers in Developing Countries: Addgene and Seeding Labs Team Up
TypeBlog PostPublishedApril 5, 2016, 2:30 p.m....lab equipment to carefully-screened research and teaching departments at universities around the world. ...the Addgene Blog Learn About Our Recent Visit to China Read Zafira Castano Corsino’s Recent Post on the... -
The Developmental Studies Hybridoma Bank: Over 25 Years of Antibody Sharing
TypeBlog PostPublishedMarch 10, 2015, 2:25 p.m.... DSHB was created by the National Institute for Child Health and Human Development (NICHD) in 1986, to...toxic adjuvants; 2) generating complex antibody chips that allow assessment of many protein targets in... -
Addgene Stands with the Scientific Community
TypeBlog PostPublishedApril 23, 2025, 1:30 p.m....establish.These funding decisions will have far-reaching consequences throughout the scientific community... can turn to Addgene as a trusted partner for archiving their biological materials during funding transitions... -
Addgene's Top Blog Posts from 2020
TypeBlog PostPublishedDec. 29, 2020, 2:15 p.m.... Plasmids 101: Plasmid Incompatibility by Leah Schiesow If you're thinking about hosting multiple plasmids...incompatible. 4. RNA Extraction Without A Kit by Leah Schiesow We added to our protocol collection this year... -
Bringing Sustainable Practices to the Lab: Innovations
TypeBlog PostPublishedMarch 7, 2019, 2:39 p.m....contamination, but consider flow cytometers. These fluidic machines handle biological samples and are cleaned between...between each use. You wouldn’t throw away your FACS machine after a single use. So how about washing and reusing... -
Our Most Popular Blog Posts from 2019
TypeBlog PostPublishedDec. 31, 2019, 2:26 p.m....Making the Right Connection by Joachim Goedhart Guest blogger Joachim Goedhart shares some strategies... -
Easing the Protein Purification Process with pCri
TypeBlog PostPublishedJune 19, 2015, 3:08 p.m....Beyond E. coli: purifying from Bacillus subtilis and Pichia pastoris pCri also includes plasmids designed for...specifically the bacterium Bacillus subtilis and the yeast Pichia pastoris. The improved secretory capacities of ... -
CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]
TypeBlog PostPublishedFeb. 18, 2015, 3:09 p.m.... by Daniel Bauer and Matthew Canver of Boston Children’s Hospital and Harvard Medical School. Addgene ...hematologist and principal investigator at Boston Children’s Hospital and Harvard Medical School. His lab ... -
Tag Your Favorite Yeast Genes with Ease
TypeBlog PostPublishedNov. 19, 2013, 2:37 p.m.... in epitope tags? Others may be interested in attaching epitope tags to their genes of interest, allowing... developed in the labs of Anne Robinson, Eishi Noguchi, and Melissa Moore, to name a few. Have you used... -
Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies
TypeBlog PostPublishedNov. 27, 2019, 2:03 p.m.... Delivery of AAV into the brain can usually be achieved by a directed brain injection, but some serotypes...types. Specific transgene expression can be also achieved in nontransgenic animals by using cis regulatory... -
pSiM24: Simplifying Plant Genetic Engineering
TypeBlog PostPublishedSept. 29, 2015, 2:30 p.m....shortened the T-DNA borders to 25 bases each. By switching the E. coli origin of replication and codon-optimizing...codon-optimizing the Agrobacterium ORI, they achieved much higher DNA yield and transformation efficiency in... -
Mapping the 4D nucleome with CRISPR/Cas9
TypeBlog PostPublishedAug. 11, 2015, 2:30 p.m....been adapted to fluorescently label a sequence matching its gRNA, facilitating the live study of chromatin...under lower illumination settings, lowering photobleaching and phototoxicity concerns and extending the... -
The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins
TypeBlog PostPublishedJuly 29, 2014, 6:16 p.m....fluorescent proteins are, which makes mixing and matching a relatively easy proposition, explained Michelle...backbone and making fusions, see this blog post from Joachim Goedhart: Fluorescent Proteins 101: GFP Fusion ... -
In Living Color: The Skinny on In Vivo Imaging Tools
TypeBlog PostPublishedMarch 27, 2014, 5:14 p.m....-infrared range, and that’s exactly what iRFPs achieved. Those proteins allowed a signal-to-background...conventional FPs. In addition, PA FPs can improve the achievable signal-to-background ratio, thus, allowing higher... -
SunTag and Fluorescent Imaging
TypeBlog PostPublishedMarch 28, 2017, 2:30 p.m....a higher signal-to-noise ratio with a lower photobleaching rate. Given the power of SunTag, they attempted... ratio Less chance of phototoxicity Reduced photobleaching Simplified, long-term single-molecule tracking... -
Sensing Neuronal Dopamine
TypeBlog PostPublishedJuly 30, 2019, 12:59 p.m.... tool to detect and image DA in live animals (Patriarchi et al., 2018). Similar to calcium and glutamate...PMID: 4403305. PubMed Central PMCID: PMC426888. Patriarchi, Tommaso, et al. "Ultrafast neuronal imaging ...