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We narrowed to 662 results for: CLU

Showing: 221 - 240 of 662 results
  1. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    Published
    Nov. 6, 2015, 3:30 p.m.
    ...mentioned above. The initial IMP cohort of 78 mentees included: 47 women (60.2%) 20 graduate students (25.6%...directly attributable to participation in the program included improvements in writing fellowships, giving oral...impressions of the IMP experience. Conference highlights included remarks by the Spanish Secretary of Research, ...
  2. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    Published
    Aug. 5, 2025, 1:15 p.m.
    ...reproducibility of experiments and the validity of conclusions. Antibody engineering also benefits antibody-...antibody. (B) Examples of engineered antibodies, including chimerization, conjugation, and antibody fragments...There are different ways to achieve this goal, including error-prone PCR, degenerate primers, gene shuffling...
  3. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...phenotype in order to conclude that the phenotype is due to an on-target effect. Conclusions Selection of gRNAs...modification of interest, it would be unwise to make conclusions on the basis of the activity of a single gRNA...
  4. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...Wild-type A549 cells do not express CAs9 and are included as a control (WT). Vinculin is a loading control... assay before, consider following their tips, including: Before digesting the DNA duplexes with Surveyor...PAGE instead of agarose to get sharper bands. Conclusion Overall, making a human cell line with a gene...
  5. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ...this time, it is! Factorial ANOVAs include two-way ANOVAs but can include many more than two independent ... Of course, a one-tailed test also works for exclusively less-than comparisons. Paired and unpaired t-tests...
  6. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ..., 1998), this 15 amino acid peptide contains a cluster of four cysteines (CCXXCC, where X is any non-cysteine...with minimal cross-reactivity. Other strategies include using engineered ligases to attach a ligand to ...study your gene of interest with these tools. Conclusion Researchers and vendors continue to develop new...
  7. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...on immune system Conventional cancer therapies include radiation therapy (owing to contributions from ...blocking. Other molecular targets under research include TIM-3, LAG-3, STAT-3, EGFR, CD20 and CD73 on specific... factors researchers are beginning to focus on include: Cell heterogeneity Tumors are composed of a wide...
  8. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...Science is about fighting scientific partisanship, including more scientists in our leadership, and raising... Anschutz Medical Campus.  He studies viruses including HIV and is interested in global health policy....Committee about climate change. All the scientists – including global warming skeptics – insisted that more data...
  9. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...impediments. FRET has been used in many applications, including drug discovery by big pharma (i.e. HTRF and LanthaScreen...dynamic range of your assay. These experiments include producing a positive control construct where the... Women’s Hospital in Boston, MA. His interests include developing novel fluorescent tools for labeling...
  10. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    Published
    Nov. 3, 2015, 3:30 p.m.
    ...2014, tools began moving away from designing exclusively for NGG PAM targets and began allowing for arbitrary... and return later or share them. Community exclusivity: Different communities develop software for their...software is organism agnostic. Most tools are exclusive to one or a subset of organisms. Examples: “organism...
  11. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...Scorpions Low Probe is on a hairpin structure included in the 5’ primer Yes Yes. Agarose gel  ...Low Reporter and quencher on hairpin structure included in primer Yes Yes Agarose gel Table...well-to-well normalization of signal. They are often included in commercial sets and most machines have a protocol...
  12. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...find the original publication and ensure that it includes validation data relevant to your application —... makes the choice easy! Protein-based blockers include dry non-fat milk or serums like FBS, PBS, and BSA...typically spans 2–5 orders of magnitude, which includes a wide dynamic range for fluorescent proteins....
  13. CRISPR 101 eBook...en español

    Type
    Blog Post
    Published
    Jan. 18, 2024, 2:15 p.m.
    ...Addgene tiene una gran audiencia internacional, incluido trabajando con distribuidores de todo el mundo...  Addgene has a large international audience, including working with distributors across the world to ...
  14. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ... there! Completed blog posts are also great to include on CVs and Resumes. An Expanded Network - Addgene...many different people. Recent Guest Writers Have Included: Wenning Qin, Molecular biologist with Biogen ...
  15. Our New Antibody Mascot is....

    Type
    Blog Post
    Published
    Feb. 24, 2022, 2:15 p.m.
    ...hunts. In fact, they’ve been following a set of clues that has led them to their biggest discovery yet...name! Additionally, anyone whose submission is included in the final four will receive some rockin’ newly...
  16. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    Published
    May 2, 2024, 1:15 p.m.
    ...primarily been performed in activated T cells, precluding analyses of T cell differentiation beginning ...also reveal novel pathways. The FITS deposit also includes pLX_305_Ovalbumin, a plasmid that enables introduction...
Showing: 221 - 240 of 662 results