...produce complex fluorescent reporter constructs. These fluorescent reporters can be used to detect structure...protein sequences to develop diverse fluorescent reporters is a useful application of high-throughput cloning...

...pathway is ubiquitous, with successful recombination reported in all laboratory E. coli strains tested so far...use of this pathway as a cloning tool was first reported over 25 years ago (Douglas and Howard, 1991, Bubeck...exploiting bacterial in vivo assembly." Scientific reports 6 (2016): 27459. PubMed PMID: 27264908. PubMed ...

...research uses ancestry groups rather than self-reported race, it highlights the myriad issues researchers...designed a tool that could analyze CRISPR sgRNAs and report the likelihood of any given sgRNA having an ancestral...complicating the issue is the long and ugly history of reporting false essentialist links between biology and ancestry...

...vector with a reporter, such as a fluorescent protein, to visualize vector uptake. These reporters might be...t common for simple transfection experiments. Reporters Finally, in some cases a researcher may choose...

...collaborative team from the David Liu and Dali Li labs reported a similar tool shortly thereafter, using mouse...substrates. The HuidaGene Therapeutics team has deposited their editors, called gTBE and gCBE, with Addgene...Transversion Editors Read about four base editor reporters Resources on Addgene.org Browse CRISPR plasmids...

...primary antibody respectively. Avidin bound to a reporter is then added to the reaction for easy detection... experiments. During detection, a biotinylated reporter enzyme that is pre-incubated with free avidin ...protein of interest leading to amplification of the reporter signal and easier detection. This strategy is ...

... Team is responsible for guiding new deposits into the repository, performing quality control, and providing...kit creation and quality control efforts with depositors; Gizela, the lab member who knows how the physical...

...and were suggested to be the best gRNAs by the depositing lab. The different labs use different algorithms... for West-Nile-virus-induced cell death." Cell reports 12.4 (2015): 673-683. PubMed PMID: 26190106. PubMed...therapeutic targets in acute myeloid leukemia." Cell reports 17.4 (2016): 1193-1205. PubMed PMID: 27760321. ...

... tRNA suppression plasmids are available from depositing labs that have mastered this technique so it ...measurements in intact cells. The Gradia Lab has deposited a gateway vector with Addgene, where you can insert...protein kinase dynamics in living cells with FRET reporters." Methods 40.3 (2006): 279-286. PubMed PMID: 16908183...

... is not efficient at the protein level at the timepoint studied Off-target activities of the RNAi Correctly... need to study protein expression at multiple timepoints. When the target of interest is critical to cell... specific detection of human TRPA1. Scientific Reports, 9(1), 18500. https://doi.org/10.1038/s41598-019...

...high is based on the reprogramming efficiencies reported in González et al. A percentage is not listed ...comprised of OCT4, SOX2, LIN28, NANOG, and a GFP reporter. Minicircles are removed from cells by dilution... iPSCs Find gene-specific plasmids (including reporters and RNAi) for:  NANOG  OCT4 SOX2 MYC KLF4 LIN28...

...first CRISPR plasmid deposit. Now we have over 8,000 CRISPR plasmids in the repository that have been shared...

...el acceso global a los materiales de nuestro repositorio. Este increíble regalo permitirá que más personas...help increase global access to materials in our repository. This incredible gift will allow more people ...

...rabies materials! Some of the materials in our repository, including rabies plasmids, are export-controlled...tools for AAV tracing, made from rabies plasmids deposited by Ian Wickersham’s lab.   ...

...kb DNA molecular weight ladders were recently deposited with Addgene. A team of undergraduate students...Generating DNA Molecular Weight Markers. Scientific Reports,7(1). doi:10.1038/s41598-017-02693-1. PubMed PMID...

...plasmids you’d like to deposit with Addgene, be sure to visit addgene.org/deposit. Who knows, maybe yours...

...ground and what the founders hope to see in the repository’s future. If you’ve ever been curious about what...distribution!) Chapter 8 - Opportunities to create repositories with other materials Chapter 9 - The Founders...

...Milling and Markson et al., 2024), developed and deposited at Addgene by Arlene Sharpe’s lab, enables in ...system can also reveal novel pathways. The FITS deposit also includes pLX_305_Ovalbumin, a plasmid that...

...If you have a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in...Find CRISPR plasmids by function Find recently deposited plasmids ...

...responsible for encoding GFP, and, in 1992, he reported its sequence. Soon after that, in 1994, Prasher...he lived! His hunch paid off, and he was able to report new fluorophores like DsRed from Discosoma sp. ...