We narrowed to 307 results for: GAI
-
TypeBlog PostPublishedSept. 24, 2024, 1:15 p.m....screens of AsLOV2 variants showed some significant gains! After a few more rounds of mutagenesis, they eventually...
-
PCR Applications for Copy Number Variation Assays
TypeBlog PostPublishedApril 26, 2022, 1:15 p.m....price point, which should be carefully balanced against the limitations of the assay. Fig.... -
Transferable Skills: Negotiation
TypeBlog PostPublishedMarch 3, 2022, 2 p.m.... company. How can I negotiate with my manager to gain experience in the area I want to explore while also... -
Of Myc and Men
TypeBlog PostPublishedJan. 19, 2023, 2:15 p.m....over the years, several alternative antibodies against the Myc tag have been generated in an attempt to... -
xCas9: Engineering a CRISPR Variant with PAM Flexibility
TypeBlog PostPublishedMarch 28, 2018, 6:52 p.m....were capable of cleavage, they tested the clones against an NNN PAM library in bacteria where cleavage leads... -
Addgene's Cancer Collection Pages Connect the Research Community
TypeBlog PostPublishedSept. 6, 2016, 2:30 p.m.... right places. Checks and balances: protecting against cancerous cells Luckily, there are several safety... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog PostPublishedAug. 4, 2016, 2:30 p.m.... and development before it can truly be pitted against CRISPR head-to-head. Exciting features of NgAgo... -
Genome-wide Screening Using CRISPR
TypeBlog PostPublishedAug. 18, 2015, 2:30 p.m....experiment can shed light on the mechanism by which cells gain resistance to drugs and can identify future therapeutic... -
Tips for Using BLAST to Verify Plasmids
TypeBlog PostPublishedMay 29, 2014, 1:29 p.m....result – blastx checks all six possible frames against the database. Another benefit is that a frameshift... -
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection
TypeBlog PostPublishedDec. 12, 2013, 3:35 p.m....other words, these parts can be used over and over again in combination with novel genes to get very precise... -
SciComm: Taking Science to Elementary and Middle Schools
TypeBlog PostPublishedJuly 9, 2019, 12:52 p.m....jobs, and an enthusiasm for coming back and trying again next year. There are many reasons why you might ... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog PostPublishedAug. 27, 2020, 1:15 p.m....localization of BiFC constructs should also be compared against those of their endogenous counterparts (by immunocytochemistry... -
What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry
TypeBlog PostPublishedJuly 1, 2025, 1:15 p.m.... off starting from the beginning. Before trying again, revisit your purification protocol. Are any of ... -
Negative Can Be Positive: Open AAV Data with Addgene
TypeBlog PostPublishedSept. 6, 2022, 1:15 p.m....D-F) High-magnification images immuno-stained against dopamine beta-hydroxylase (DBH, magenta) to label... -
An Introduction to Adenovirus
TypeBlog PostPublishedOct. 3, 2023, 1:15 p.m.... Vectors Overcome Preexisting Humoral Immunity against Human Adenoviruses In Vivo. J Virol, 73(8), 6930... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog PostPublishedJuly 27, 2023, 1:15 p.m....emerges. Retrovirus life cycle Retroviruses go against the grain of the Central Dogma of Biology (gDNA... -
Getting to Know Expansion Microscopy
TypeBlog PostPublishedJune 20, 2023, 1:15 p.m....arrived on the scene, ExM has helped researchers gain a deeper understanding of neuronal synapses, centrosomes... -
Great Results Start with Great Standard Curves
TypeBlog PostPublishedAug. 15, 2024, 1:15 p.m....concentration of a serially diluted standard is plotted against its OD560 measurements and the trendline y = 0.6561x... -
Antibodies 101: Producing Recombinant Antibodies
TypeBlog PostPublishedAug. 13, 2024, 1:15 p.m.... density of the antibody bands can be measured against the protein standard and the concentration may ... -
A Guide to Starting Your Own Journal Club
TypeBlog PostPublishedFeb. 6, 2020, 3 p.m....the questions “Would you attend this journal club again?” and “How would you improve the structure of the...