We narrowed to 303 results for: GAI

1. AAV Vector Quality Control: Going the Extra Mile with NGS

   Type

   Blog Post

   Published

   Sept. 12, 2017, 1:44 p.m.

   ...to the contaminant. Each contig is then BLASTed against the NCBI nucleotide collection database, and it...

2. Cloning Mammalian Cells with the Agarose Method

   Type

   Blog Post

   Published

   Sept. 7, 2017, 12:17 p.m.

   ...We test the (capped) 1:1 agarose/DMEM solution against a part of the body very sensitive to heat- the ...

3. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

   Type

   Blog Post

   Published

   June 22, 2017, 2:30 p.m.

   ...However, much knowledge about functionality can be gained by understanding the location and transport of ...

4. Addgene's Cancer Collection Pages Connect the Research Community

   Type

   Blog Post

   Published

   Sept. 6, 2016, 2:30 p.m.

   ... right places. Checks and balances: protecting against cancerous cells Luckily, there are several safety...

5. Google Forums Round Up: First Impressions of NgAgo

   Type

   Blog Post

   Published

   Aug. 4, 2016, 2:30 p.m.

   ... and development before it can truly be pitted against CRISPR head-to-head.  Exciting features of NgAgo...

6. Genome-wide Screening Using CRISPR

   Type

   Blog Post

   Published

   Aug. 18, 2015, 2:30 p.m.

   ...experiment can shed light on the mechanism by which cells gain resistance to drugs and can identify future therapeutic...

7. Tips for Using BLAST to Verify Plasmids

   Type

   Blog Post

   Published

   May 29, 2014, 1:29 p.m.

   ...result – blastx checks all six possible frames against the database. Another benefit is that a frameshift...

8. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

   Type

   Blog Post

   Published

   Dec. 12, 2013, 3:35 p.m.

   ...other words, these parts can be used over and over again in combination with novel genes to get very precise...

9. Getting to Know Expansion Microscopy

   Type

   Blog Post

   Published

   June 20, 2023, 1:15 p.m.

   ...arrived on the scene, ExM has helped researchers gain a deeper understanding of neuronal synapses, centrosomes...

10. A Guide to Starting Your Own Journal Club

    Type

    Blog Post

    Published

    Feb. 6, 2020, 3 p.m.

    ...the questions “Would you attend this journal club again?” and “How would you improve the structure of the...

11. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type

    Blog Post

    Published

    Dec. 3, 2019, 11:38 a.m.

    ...plasmid from the NeuroMab collection for an antibody against PARIS, a zinc finger protein involved in neurodegeneration...

12. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type

    Blog Post

    Published

    Aug. 27, 2020, 1:15 p.m.

    ...localization of BiFC constructs should also be compared against those of their endogenous counterparts (by immunocytochemistry...

13. Tips for arabidopsis transformation

    Type

    Blog Post

    Published

    Oct. 25, 2018, 1:23 p.m.

    ... let them recover for a week, and then dip them again.  This won’t hurt them, and it will increase the...

14. Simplify Cloning with in vivo Assembly

    Type

    Blog Post

    Published

    Oct. 18, 2018, 12:37 p.m.

    ...et al., 1993). But why did this technique fail to gain traction? It is likely that both vector amplification...

15. SciComm: Taking Science to Elementary and Middle Schools

    Type

    Blog Post

    Published

    July 9, 2019, 12:52 p.m.

    ...jobs, and an enthusiasm for coming back and trying again next year. There are many reasons why you might ...

16. Negative Can Be Positive: Open AAV Data with Addgene

    Type

    Blog Post

    Published

    Sept. 6, 2022, 1:15 p.m.

    ...D-F)  High-magnification images immuno-stained against dopamine beta-hydroxylase (DBH, magenta) to label...

17. AAVs in Retinal Gene Therapy

    Type

    Blog Post

    Published

    July 31, 2018, 12:55 p.m.

    ...emerges from disgrace to be the next big thing, again 3. Acland G. et al., 2001. Gene therapy restores...

18. An Introduction to Adenovirus

    Type

    Blog Post

    Published

    Oct. 3, 2023, 1:15 p.m.

    ... Vectors Overcome Preexisting Humoral Immunity against Human Adenoviruses In Vivo. J Virol, 73(8), 6930...

19. Viral Vectors 101: The Retroviral Lifecycle

    Type

    Blog Post

    Published

    July 27, 2023, 1:15 p.m.

    ...emerges. Retrovirus life cycle Retroviruses go against the grain of the Central Dogma of Biology (gDNA...

20. Great Results Start with Great Standard Curves

    Type

    Blog Post

    Published

    Aug. 15, 2024, 1:15 p.m.

    ...concentration of a serially diluted standard is plotted against its OD560 measurements and the trendline y = 0.6561x...