We narrowed to 254 results for: IRES

...Whereas Southern blotting is labor intensive and requires a large amount of high-quality DNA, real-time ...

...variations and heat transfer at the subcellular level requires a probe with fast kinetics and high signal/noise...

...visualize their target. In contrast, indirect IF requires two antibodies: a primary and secondary. For indirect...

...for the scFv (Gaciarz and Ruddock., 2017), but requires an oxidizing environment to form. When expressed...

...of S. pyogenes Cas9. While the wild-type enzyme requires a PAM sequence of NGG (where N represents any ...

...housekeeping proteins.” Fully quantifying a western blot requires a standard curve with a pure sample of your protein...

...really are selling yourself. A sales pitch that requires reading two pages or even a paragraph to get to...

...instability at 37°C (Ouellet et al., 2016) and requires a tRNA scaffold to improve its stability in vivo...

... hence, intracellular labeling with antibodies requires chemically pre-treating the cells to allow for...

...to support your work. For example, if your work requires specialized equipment, try finding institutes ...

...transformants for single colonies (this usually requires 10-20 plates) (see box below). This initial transformation...

...where those interactions occur in the genome. This requires both targeting a specific genome location and ...

...experiments. A multiplexed experiment, for example, requires staining with multiple primary and secondary antibodies...

...overnight.  In contrast, a semi-dry transfer only requires enough buffer to saturate the sandwich and typically...

...sequence added, leaving a 34 bp “scar”, but currently requires additional experimental manipulation. In some ...

...and ideas toward solving one problem. It often requires thinking about a problem in a new way through ...

...amenable to high-throughput analyses, however, it requires access to a mass spec facility and the ability...

...assistants), about their experiences. Research requires dedication and effort, so building a strong understanding...

...membrane, a threshold is reached and the neuron fires, sending the electrical signal down the axon (Lodish...

...auto-fluorescence (Drobizhev et al. 2011). But this requires other lasers than the standard Ti:Sapphire laser...