We narrowed to 253 results for: IRES

1. Hot Biosensors 2022: Year-End Roundup

   Type

   Blog Post

   Published

   Dec. 6, 2022, 2:15 p.m.

   ...variations and heat transfer at the subcellular level requires a probe with fast kinetics and high signal/noise...

2. Antibodies 101: Single Chain Fragment Variables (scFvs)

   Type

   Blog Post

   Published

   June 3, 2021, 1:15 p.m.

   ...for the scFv (Gaciarz and Ruddock., 2017), but requires an oxidizing environment to form. When expressed...

3. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

   Type

   Blog Post

   Published

   May 5, 2020, 1:15 p.m.

   ...sequence preferences at the target site, while Cas12a requires a PAM (Protospacer Adjacent Motif) for target ...

4. The Fluorescent Vegetables in Aptamer Soup

   Type

   Blog Post

   Published

   Jan. 29, 2019, 2:27 p.m.

   ...instability at 37°C (Ouellet et al., 2016) and requires a tRNA scaffold to improve its stability in vivo...

5. Deep Mutational Scanning with One Pot Saturation Mutagenesis

   Type

   Blog Post

   Published

   Feb. 22, 2017, 3:30 p.m.

   ...phage-derived uracil labeled template, this method requires further DNA shuffling to combine pairs of mutations...

6. Site Directed Mutagenesis by PCR

   Type

   Blog Post

   Published

   Aug. 2, 2016, 2:30 p.m.

   ...double-stranded break. This site-directed genome editing requires a template of high homology to the endogenous ...

7. Using Phosphoserine to Study Protein Phosphorylation

   Type

   Blog Post

   Published

   June 23, 2016, 2:30 p.m.

   ... The generation of pSer-incorporated proteins requires a molecular toolkit containing the OTS, an appropriate...

8. Evolution of Lab Techniques

   Type

   Blog Post

   Published

   June 21, 2016, 2:30 p.m.

   ...Whereas Southern blotting is labor intensive and requires a large amount of high-quality DNA, real-time ...

9. How to Negotiate a Successful Lab Start Up Budget

   Type

   Blog Post

   Published

   Sept. 1, 2015, 2:30 p.m.

   ...to support your work. For example, if your work requires specialized equipment, try finding institutes ...

10. A Control for All Seasons

    Type

    Blog Post

    Published

    Oct. 5, 2023, 1:15 p.m.

    ...experiments. A multiplexed experiment, for example, requires staining with multiple primary and secondary antibodies...

11. Hot Plasmids: Spring 2025

    Type

    Blog Post

    Published

    June 3, 2025, 1:15 p.m.

    ...where those interactions occur in the genome. This requires both targeting a specific genome location and ...

12. Antibodies 101: The Basics of Western Blotting

    Type

    Blog Post

    Published

    March 8, 2021, 2:41 p.m.

    ...overnight.  In contrast, a semi-dry transfer only requires enough buffer to saturate the sandwich and typically...

13. Technique: Probe Phage Genomes for Host Binding Proteins

    Type

    Blog Post

    Published

    Sept. 29, 2016, 2:30 p.m.

    ...transformants for single colonies (this usually requires 10-20 plates) (see box below). This initial transformation...

14. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type

    Blog Post

    Published

    Oct. 17, 2019, 12:28 p.m.

    ...membrane, a threshold is reached and the neuron fires, sending the electrical signal down the axon (Lodish...

15. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type

    Blog Post

    Published

    Dec. 17, 2020, 1:30 p.m.

    ...sequence added, leaving a 34 bp “scar”, but currently requires additional experimental manipulation. In some ...

16. Finding Your Science Policy Path

    Type

    Blog Post

    Published

    Sept. 22, 2020, 1:15 p.m.

    ...and ideas toward solving one problem. It often requires thinking about a problem in a new way through ...

17. Antibodies 101: Validation

    Type

    Blog Post

    Published

    March 24, 2022, 1:15 p.m.

    ...amenable to high-throughput analyses, however, it requires access to a mass spec facility and the ability...

18. A Guide to Getting Started in Undergrad Research

    Type

    Blog Post

    Published

    June 8, 2021, 1:15 p.m.

    ...assistants), about their experiences. Research requires dedication and effort, so building a strong understanding...

19. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type

    Blog Post

    Published

    Aug. 15, 2017, 1:24 p.m.

    ...auto-fluorescence (Drobizhev et al. 2011). But this requires other lasers than the standard Ti:Sapphire laser...

20. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type

    Blog Post

    Published

    March 21, 2024, 1:15 p.m.

    ...another factor to consider. If your application requires longer periods of exposure, you’ll want a more...