We narrowed to 254 results for: IRES

... hence, intracellular labeling with antibodies requires chemically pre-treating the cells to allow for...

...double-stranded break. This site-directed genome editing requires a template of high homology to the endogenous ...

...switching from paper to an electronic lab notebook requires time and knowledge. In a recent article, we translated...

...variations and heat transfer at the subcellular level requires a probe with fast kinetics and high signal/noise...

...visualize their target. In contrast, indirect IF requires two antibodies: a primary and secondary. For indirect...

...for the scFv (Gaciarz and Ruddock., 2017), but requires an oxidizing environment to form. When expressed...

...sequence preferences at the target site, while Cas12a requires a PAM (Protospacer Adjacent Motif) for target ...

... The generation of pSer-incorporated proteins requires a molecular toolkit containing the OTS, an appropriate...

...Whereas Southern blotting is labor intensive and requires a large amount of high-quality DNA, real-time ...

...to support your work. For example, if your work requires specialized equipment, try finding institutes ...

...experiments. A multiplexed experiment, for example, requires staining with multiple primary and secondary antibodies...

...where those interactions occur in the genome. This requires both targeting a specific genome location and ...

...transformants for single colonies (this usually requires 10-20 plates) (see box below). This initial transformation...

...overnight.  In contrast, a semi-dry transfer only requires enough buffer to saturate the sandwich and typically...

...membrane, a threshold is reached and the neuron fires, sending the electrical signal down the axon (Lodish...

...amenable to high-throughput analyses, however, it requires access to a mass spec facility and the ability...

...assistants), about their experiences. Research requires dedication and effort, so building a strong understanding...

...sequence added, leaving a 34 bp “scar”, but currently requires additional experimental manipulation. In some ...

...and ideas toward solving one problem. It often requires thinking about a problem in a new way through ...

...another factor to consider. If your application requires longer periods of exposure, you’ll want a more...