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We narrowed to 615 results for: LIS

Showing: 221 - 240 of 615 results

  1. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...enrichment via enzyme-linked immunosorbent assay (ELISA) The enriched sybody pools from the phage display...the protein of interest. The Seeger lab used the ELISA technique described in their protocol because it...starts out with anti-Myc antibodies bound to an ELISA plate to capture Myc-tagged sybodies. Then, the ...detected using a colorimetric reaction. After the ELISA step, the narrowed-down pool (usually 10-30) of ...

  2. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ... is a list of poster titles or abstracts available before the conference, comb through that list and note... this is being used, it’s best to turn to an established social media platform. During the conference ...something on social media. If someone is presenting unpublished data they may ask the audience to not share their...

  3. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...well defined peptides. Munro and Pelham’s first published peptide tagging experiment involved a peptide ...recognized by Myc1-9E10 down to 10 amino acids (EQKLISEEDL) - larger than the substance P peptide, but still... antibody binds. For example, Myc1-9E10 binds EQKLISEEDL (pronounced “equi-kli-seed-le”). (3) Tag all ...limitations was described by Schüchner, et al., who published a paper showing that the amino acids neighboring...

  4. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...specific cell types (Rouet et al. 2018). This was accomplished using a Cas9 mutant (Cas9M1C/C80S) that harbors...been an area of intense study. Two methods to accomplish this are Polyethylene Glycol (PEG) mediated transfection... transfection of protoplasts and biolistic bombardment of immature embryos (Liang et al. 2017).  PEG-mediated... to perform, often show low efficiency, while biolistic bombardment, which can have high efficiency, requires...

  5. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    Published
    Dec. 3, 2019, 11:38 a.m.
    ...’ button a new content-aware suggestion dropdown list will let you jump directly to the type of information...to the plasmid page at the bottom of the dropdown list: The search bar will suggest direct links to other... antibody plasmids from the James Trimmer Lab published in eLife. Addgene is currently distributing 94...the ‘X’ next to any filter to clear it. Here’s a list of available filters to refine your search results...

  6. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ... Protein tags are usually smallish peptides incorporated into a translated protein. As depicted in the...purification with amylose, but wicked huge Myc EQKLISEEDL  1.2 Affinity Frequently used for western blots...tags and fusion proteins as examples. The tables list more common tags than are described in the post,...Journal 2012 , (7): 620-634. Full Text A complete list of free handbooks for protein purification is provided...

  7. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...fluorophores are constantly being engineered. Table 1 lists a few common fluorescent proteins and their mutations...mutations relative to wildtype GFP. Although not listed here, many permutations within each color also exist...plasmids as a means to many functional ends. We’ve listed our favorites below, but many other uses currently...Haimovich of greenfluorescentblog. Gal will share his list of 10 things to keep in mind when choosing the best...

  8. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...reversible. Late last year, the Akhmanova Lab published a new optogenetic tool for localized microtubule...transport, and cell division, but given the long list of roles played by microtubules, Opto-katanin will...understand their roles in forming the cilia that help establish left-right organization of the embryo (Aljiboury...the membrane itself? The Baskin Lab previously published OptoPLD, an optogenetically controlled phospholipase...

  9. Help Us Name Blugene’s New Virus Friend

    Type
    Blog Post
    Published
    Jan. 7, 2020, 1:55 p.m.
    ... contest is now closed. Stay tuned to see the finalists and vote for your favorite! Our mascot who has...neutral name. The Addgenies will choose up to four finalists and we’ll ask you to vote for your favorite name...We hope you submit your best ideas because the finalists will receive some newly designed Addgene swag....

  10. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...Mammalian Cells Note: This is not an extensive list Acceptor labeling: Sometimes it's okay to be wrong...However, proper control experiments are needed to establish the dynamic range of your assay. These experiments...energy transfer values you can expect, thereby establishing the dynamic range of your FRET assay (Figure... developed, so don’t be afraid to try novel, unpublished combinations of labeling strategies. FRET experimental...

  11. Addgene at SfN23!

    Type
    Blog Post
    Published
    Nov. 9, 2023, 2:15 p.m.
    ...Addgenies! We'll have an antibody specialist, Amrita, and a viral vector specialist, Rebecca. They will be working...is rounded out with our science communication specialist (you'll know me by my Tweeting — er, posting!...

  12. Imaging Tools and Following a Passion for Basic Biology: Interview with Joachim Goεdhart

    Type
    Blog Post
    Published
    March 9, 2018, 2 p.m.
    ...helped us update many of our educational resources. Listen to hear all about Professor Goedhart, his lab, ..., and some of the tools he’s developed.   Listen to the interview with Joachim Goedhart Podcast breakdown... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer...

  13. SciComm with the Experts at Science in the News Part 1

    Type
    Blog Post
    Published
    March 16, 2017, 1:06 p.m.
    ...learn how they think about communicating science. Listen to the interview with Science in the News, Part...Non-Scientists How to Approach Different Audiences Listen to the SITN Podcast Many thanks to our guests,... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer...

  14. Activate Addgene's "Find Plasmids" Badge on Pubmed Abstracts

    Type
    Blog Post
    Published
    May 12, 2017, 12:58 p.m.
    ...you ever thought - Is there an easy way to find published plasmids? Do you spend a lot of time in PubMed...This selection should populate your “PubMed filter list” to the left of your screen. That’s it! Next time...to set-up emails to alert you when papers are published on your favorite topic. If you have any other...

  15. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

    Type
    Blog Post
    Published
    Nov. 15, 2016, 3:30 p.m.
    ...board where he will help shape Addgene's future. Listen to this episode for insights into the process of... tools, and the potential uses of gene drives. Listen here! Podcast breakdown (Time : Topic) 0:00 - 2... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer...

  16. Kwabena Duedu on Public Health, Biofuels, and Doing Science in Ghana

    Type
    Blog Post
    Published
    Dec. 20, 2016, 3:30 p.m.
    ...some time learning and doing research at Novartis. Listen to learn how Kwabena plans to use his research ...opportunities to Ghanaian scientists Click here to listen! Podcast breakdown (Time : Topic) 0:00 - 7:11 :... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer...

  17. Bringing Sustainable Practices to the Lab: Easy Action Items

    Type
    Blog Post
    Published
    April 22, 2019, 12:26 p.m.
    ... on sustainable practices in the lab, where I’ll list some easy but effective habits you can incorporate...long-term goals to stay motivated and to feel accomplished. In addition to doing some of the quick things...has a lot of resources about this, and even has a list of biological samples that are stored long term ...

  18. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    Published
    Oct. 6, 2016, 2:30 p.m.
    ...great advice on all of these topics. Listen to Part 1 Here Listen to Part 2 here! Time : Topic 0:00 - ... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer...

  19. Michael Koeris' Journey from Grad Student to Entrepreneur: The Story of Sample 6

    Type
    Blog Post
    Published
    Jan. 21, 2016, 4:52 p.m.
    ...transition from graduate student to entrepreneur, listen to our full interview with Dr. Koeris below. Check... specific subjects in the interview and happy listening! Download the podcast here! Podcast breakdown ... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer...

  20. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    Published
    Feb. 27, 2020, 2:15 p.m.
    ...simple as possible. We are the Customer Support Specialists here at Addgene. If you’ve called our customer... purchase order, which is an official document listing materials and pricing information that is sent ...send an invoice to the billing address that you listed during checkout. This invoice will reference your...
Showing: 221 - 240 of 615 results