We narrowed to 287 results for: PREP

1. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

   Type

   Blog Post

   Published

   June 25, 2019, 12:54 p.m.

   ...and AAV plasmids as well as ready-to-use viral preparations at Addgene. For more information on viral vectors...

2. A Deep Dive into BioBuilder

   Type

   Blog Post

   Published

   Oct. 11, 2017, 1:44 p.m.

   ... The BioBuilder High School Apprenticeship, preparing local (Massachusetts), economically disadvantaged...

3. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

   Type

   Blog Post

   Published

   July 6, 2017, 1:59 p.m.

   ...reflect how cancer/immunology researchers are preparing and carrying out this multi-pronged attack. Inflammation...

4. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

   Type

   Blog Post

   Published

   Feb. 18, 2014, 4:22 p.m.

   ...pre-reads, videos or exercises to be completed in preparation for the meeting or to be read or watched during...

5. Will You Be My Mentor? Finding and Asking for Mentoring Support

   Type

   Blog Post

   Published

   Feb. 4, 2014, 3:50 p.m.

   ...Thank them anyway and move on. If they say yes, be prepared with questions and topics to discuss. The mentee...

6. Transferable Skills Guide: Cross-team Communication

   Type

   Blog Post

   Published

   Nov. 21, 2019, 1:53 p.m.

   ...miscommunication and b) find practical solutions.  My PhD prepared me remarkably well for this task. Good scientists...

7. Troubleshooting Your Plasmid Cloning Experiment

   Type

   Blog Post

   Published

   Sept. 24, 2019, 12:55 p.m.

   ...strain is growing fine, it is probably time to prepare new plates with a fresh stock of antibiotic. Many...

8. Plasmid Tools for Microbiome Studies

   Type

   Blog Post

   Published

   June 27, 2019, 12:25 p.m.

   ...distribution of gut bacteria without fixation or preparation, and deliver CRISPR components for gene disruption...

9. Human Germline Editing Using CRISPR

   Type

   Blog Post

   Published

   Aug. 10, 2017, 2:19 p.m.

   ...Note: After this blog was published, a bioRxiv preprint that questions the conclusion of inter-homologue...

10. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type

    Blog Post

    Published

    June 15, 2017, 2:30 p.m.

    ...publishing it as (part of) figure 1, uploading it as a preprint, or documenting it in some other way. Besides ...

11. Antibodies 101: Secondary Antibodies

    Type

    Blog Post

    Published

    Dec. 21, 2021, 2:15 p.m.

    ...architecture of the indirect approach is as follows: Prepare the sample. Incubate the sample with the primary...

12. Transferable Skills Guide: Problem Solving

    Type

    Blog Post

    Published

    Nov. 30, 2021, 2:15 p.m.

    ... of contexts. As such, scientists are uniquely prepared to be strong problem solvers both in and out of...

13. Antibodies 101: Selecting the Right Antibody

    Type

    Blog Post

    Published

    March 15, 2022, 1:15 p.m.

    ...different samples and experimental conditions. Be prepared to troubleshoot and be sure to include proper ...

14. Addgene's Tips for Plasmid Quality Control

    Type

    Blog Post

    Published

    Jan. 14, 2016, 3:30 p.m.

    ... over 2000 primers. Two 96-well PCR plates are prepared with the primer stocks for forward and reverse...

15. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type

    Blog Post

    Published

    May 5, 2015, 3:54 p.m.

    ...subscription), archived on PubMed Central, or in preprint onBioRXIV. One of the biggest issues you will ...

16. Making CRISPR Plasmids Using Fragmid

    Type

    Blog Post

    Published

    Aug. 29, 2024, 1:15 p.m.

    ...always, we recommend checking your cloning via a miniprep and restriction digest, and then plasmid sequencing...

17. Antibodies 101: Stripping and Reprobing Western Blots

    Type

    Blog Post

    Published

    Aug. 1, 2024, 1:15 p.m.

    ...that section of the blot unusable for reprobing. Preparing your western blot Run your gel and membrane transfer...

18. Engaging with science and society at pgEd

    Type

    Blog Post

    Published

    Sept. 20, 2018, 12:17 p.m.

    ...running these events will be important both in preparing you for future work in these areas, and in helping...

19. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type

    Blog Post

    Published

    May 24, 2018, 1:20 p.m.

    ...riboprobes are created, the WISH protocol involves preparation of the tissue sample, several washes, and temperature...

20. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type

    Blog Post

    Published

    May 22, 2018, 1:38 p.m.

    .... cerevisiae met deficiencies (manuscript in preparation), indicating that Met protein functions have ...