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We narrowed to 524 results for: PROC

Showing: 221 - 240 of 524 results

  1. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ... start studying up before you begin the hiring process. Read Genevive Bjorn's "Ready, Set, Hire" on Nature...home message Starting a lab can be a challenging process. Each PI emphasizes the importance of slowly building...

  2. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...material (such as plasmids) into mammalian cells, a process called transfection. Transfection is somewhat comparable.... Since the DNA introduced in the transfection process is not integrated into the nuclear genome, in the...

  3. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    Published
    Aug. 20, 2024, 1:15 p.m.
    ...intensity. If your samples and controls were processed the same way, the GFP- population should overlap...samples and control underwent different treatment processes, you may see a shift in the negative population...

  4. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    Published
    Sept. 15, 2020, 1:15 p.m.
    ...analyze plasmids, which can help you visualize this process, or visit our restriction analysis blog post. ...now use next-generation sequencing during our QC process. This way, we can confirm the sequence of the entire...

  5. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...factors as proxies for a cellular process or directly measure a cellular process. Cellular phenomena such as...

  7. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ... the nick site, which was expected since the processivity window of PolI is 15-20 bp. EvolvR’s on-target...bacteriophage T7 DNA polymerase increased the processivity of PolI3M, and thereby increasing the editing...

  10. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ...quickly.  Through the years, we have perfected processes to curate the data our depositors share and enrich...and handle millions of queries. In an iterative process driven by our user experience experts, our software...

  11. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    Published
    June 30, 2016, 2:30 p.m.
    ...protein expression. Methylases involved with these processes (for example Dam and Dcm methylases) are independent...used for site directed mutagenesis. During this process, incorporation of a desired mutation into your ...

  12. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...understand, and the complicated and unique metabolic processes of plants are being investigated on limited funding... developmental, metabolic, and photosynthetic processes. Often the tools developed for plant molecular...

  13. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ... see how those mutations influence the disease process. In the Cell Stem Cell paper, for example, we generated... increase the efficiency of the genome editing process. The first really popular tool was the zinc finger...

  14. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...meant facilitating the Material Transfer Agreement process, which was a major barrier for depositors and requestors... requestors alike. We transformed this process by aligning institutions with standardized agreements and...

  15. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...you with some advice on the antibody selection process. Antibodies are not one size fits all When choosing...experiments are the same. Differences in sample processing, fixation, antigen-retrieval, and permeabilization...

  17. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    Published
    April 20, 2023, 4 p.m.
    ...your ideas, invite others into decision making processes, and even interview better! As a scientist turned..., re-oriented, continued presenting. The whole process took just under a minute. I can't say the journal...

  19. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...still express ccdB and therefore would die. This procedure dramatically reduces the number of clones that...recombinant plasmid and therefore makes the cloning process much more efficient, as one does not have to thoroughly...

  20. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ... massively parallel combinatorial genetics." Proceedings of the National Academy of Sciences 111.34 (2014... CRISPR-Cas9 screening enabled by CombiGEM." Proceedings of the National Academy of Sciences113.9 (2016...
Showing: 221 - 240 of 524 results