Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 599 results for: REV;

Showing: 221 - 240 of 599 results

  1. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...(Pfeiffer et al., 2010). Yeast (Saccharomyces cerevisiae) Yeast, one of the simplest eukaryotic organism...190-195. Liu, Wei, et al. "From Saccharomyces cerevisiae to human: The important gene co‑expression modules...in therapeutic drug discovery." Pharmacological reviews 63.2 (2011): 411-436. PubMed PMID: 21415126. PubMed...

  2. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ...efficiently by (1) improving editing accuracy by preventing re-editing and (2) optimizing “cut-to-mutation... was that these “blocking mutations” mutations prevent CRISPR from re-cutting the target sequence once... mutation incorporation probabilities our data revealed, and found that for generating a homozygous HDR...

  3. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 3:42 p.m.
    ...rAAVs, can sound almost too good to be true! In a previous post, we covered systemic capsids, which allow...1, rAAV2/2, and so on, although we often use abbreviated nomenclature (AAV1, AAV2, etc.) to distinguish... known as AAV2-retro) We’ve covered AAVrg in a previous blog post, but to summarize briefly: AAVrg is ...

  4. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...cells compared to monomers, although this can be prevented via antibiotic selection (Crozat, 2014). How to... multimerization, and in some cases completely prevent the formation of plasmid multimers (Bedbrook & ...overgrowing the bacterial culture Another way to prevent plasmid multimerization is to avoid growing the...

  5. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ...gathering data. Former lab members can give open, revealing information about the culture and lab head. While...intimidation, sexual harassment Isolation – including preventing access to opportunities, physical or social isolation...record notes and observations so you have data to review when making a decision. If you don’t see a culture...

  6. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...biased and unbiased approaches. As suggested in a review by Tycko et al., 2016, unbiased sequencing and ...High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity." Nature...specificities of CRISPR-Cas9 nucleases." Nature Reviews Genetics17.5 (2016): 300-312. PubMed PMID: 27087594...

  7. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...inhibitor of uracil DNA glycosylase (UGI) to dCas9 prevents base excision repair, which would change the U...of inosine from DNA is likely infrequent, thus preventing the induction of base excision repair. In terms...improved base editing capacity, even at sites previously difficult to target. ABE8s can achieve 98-99%...

  8. Enabling high school research at the Journal of Emerging Investigators

    Type
    Blog Post
    Published
    Sept. 5, 2018, 12:01 p.m.
    ...that enables high school students to publish peer-reviewed scientific research. You’ll meet some of the folks...mouth and personal networking!) and the manuscript review/submission process at JEI. Chapter 3 - JEI target...

  9. Deck the Lab 2022!

    Type
    Blog Post
    Published
    Jan. 17, 2023, 2:15 p.m.
    ...DeckTheLab and tagging us, @addgene to be judged! In previous years, scientists have decorated their labs with...on the entries, select up to three winners, and reveal the winning entries on Twitter and Instagram! Happy...

  10. Streaking for Single Colonies: The Streak Plate Challenge

    Type
    Blog Post
    Published
    March 2, 2021, 2:15 p.m.
    ...microbiology lab every year for over 20 years. Previously, the lab used three different species for the...continues until all quadrants are used, hopefully revealing single, isolated colonies in the last quadrant...

  11. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ... expression of specific genes in the brain in reversible fashion. And that’s exactly what the LITEs now...response can be graded with light dose and is fully reversible after light retraction. Finally, because light...

  14. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    Published
    June 19, 2015, 3:08 p.m.
    ... stage is often much easier said than done. As reviewed in our Plasmids 101 series, proteins are overexpressed...protein expression and purification: a comprehensive review of affinity tags and microbial applications." Biotechnology...

  15. Science as Art

    Type
    Blog Post
    Published
    March 2, 2015, 3:13 p.m.
    ...x-ray machine not on teeth, but on blossoms, thus revealing the startling inner beauty of familiar flowers...such as those collected by Darwin on his voyage. Previous destructive techniques were incompatible with ...

  16. "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring

    Type
    Blog Post
    Published
    Dec. 17, 2013, 2:47 p.m.
    ...another is not just to share your riches but to reveal to him his own." -Benjamin Disraeli Mentor, Sponsor...and more. It is instructive (and entertaining) to review this list of bad mentor behaviors. Sadly, it is...

  17. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...repression of gene expression in Saccharomyces cerevisiae. The system consists of dCas9, the mammalian ...multiplexed gRNAs in human pluripotent stem cells. Previously, CRISPRi or CRISPRa depended on continued expression...

  18. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ...assembly from cryo-electron microscopy, the team reveal the mechanism behind its activity including an ...motif, averaging 19 targets per gene. The lab has previously used the library with SpdCas9 to perform pooled...

  19. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    Published
    Sept. 29, 2015, 2:30 p.m.
    ...As previous blogs have noted, plants are an important foundation for life on Earth. Selective breeding... resistance. Much of plant genetic engineering revolves around Agrobacterium tumifaciens. Agrobacterium...

  20. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...transcriptional activation/repression. dCas9 has also previously been adapted to fluorescently label a sequence...calculated fluorescent distances correlating with the previously established physical map. In comparing pairs ...
Showing: 221 - 240 of 599 results