We narrowed to 459 results for: SHI
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TypeBlog PostPublishedSept. 24, 2024, 1:15 p.m....MagLOV. Andrew was thrilled to see that MagLOV’s “astonishing” magnetoresponse was clearly visible whether ...range of topics. He embraces open science, self-publishing directly on his website (and often posting updates...
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Viral Vectors 101: Voltage Indicators
TypeBlog PostPublishedJan. 30, 2024, 2:15 p.m....vitro. Factors like photobleaching, lack of red-shifted indicators, and brightness of fluorescence all ... significantly brighter and available with red-shifted fluorophores (find FRET capable GEVIs here!). The... -
PhD Applications After COVID
TypeBlog PostPublishedJan. 5, 2023, 2:15 p.m....the question “So, what comes next?” For those finishing up an undergraduate education or ending a Master... Bastin is a first year PhD student at George Washington University whose research focuses on Drosophila-parasite... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog PostPublishedDec. 10, 2020, 1 p.m.... as a result of an insertion, a deletion or a frameshift (Ding et al., 2014, Wang et al., 2015). In these...available at Addgene and could be useful to labs wishing to create this kind of animal models. Start using... -
The Scientific Conference Poster Session: Tips for Success
TypeBlog PostPublishedAug. 29, 2019, 12:50 p.m.... science. Prepare the night before: To avoid rushing out the door without your poster, place it by your... you may receive. People often ask about the relationship of your work to recently published papers or... -
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies
TypeBlog PostPublishedJuly 13, 2017, 2:30 p.m....fruit flies before, get yourself a copy of Fly Pushing by Ralph J. Greenspan. It’s a fantastic book for...thanks to our guest blogger, Jon Chow! Jon Chow is finishing his immunology PhD at Harvard University. He has... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog PostPublishedApril 19, 2016, 2:30 p.m....Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz at the National...1999. 17(10): p. 969-73. PubMed PMID: 10504696. 4. Ohashi, T., et al., An experimental study of GFP-based... -
Experimenting with New Careers while in Grad School
TypeBlog PostPublishedFeb. 25, 2016, 3:30 p.m.... Brain Scoop YouTube channel @Ehmee, and David Shiffman @WhySharksMatter and see what kind of content ... been the best supporter and most productive partnership CINSO has had. Volunteer! The turning point in... -
Great Results Start with Great Standard Curves
TypeBlog PostPublishedAug. 15, 2024, 1:15 p.m....fit and 0 indicating that there is no linear relationship between the observed and predicted values. This... is no correlation or it could mean that the relationship is non-linear and you should use a different... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog PostPublishedNov. 29, 2022, 2:15 p.m.... they looked for a biological explanation, a relationship between the genetic dependencies they identified... ancestries. While there were some exciting relationships identified, 48% were found to be artificial,... -
Capturing a Moment with Marc Zimmer’s “The State of Science”
TypeBlog PostPublishedJuly 30, 2020, 1:15 p.m....examines the inherent brokenness of scientific publishing and research funding. I’ve been aware of these... place. Can we address problems in scientific publishing without acknowledging the systemic sexism in ... -
Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog PostPublishedAug. 30, 2022, 1:15 p.m....maintaining the structure of your samples, the relationships between cellular components, and the accessibility...saponin. However, if you are not worried about washing away your target (for example, some cytoskeletal... -
Lentiviral Vector Uses and Overview
TypeBlog PostPublishedMay 19, 2016, 2:30 p.m....self-inactivating lentivirus vector. Miyoshi H, Blömer U, Takahashi M, Gage FH, Verma IM. J Virol. 1998... -
A Career in Grants: How To Become a Grant Professional
TypeBlog PostPublishedAug. 20, 2015, 2:30 p.m....budget narratives, logic models, established partnerships and collaborations, and commitments to and proof...close-out a grant. Grant professionals also build relationships with foundations and government grantmakers.... -
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog PostPublishedDec. 2, 2014, 7:23 p.m....Addgene takes care of everything including MTA and shipping. Addgene realizes the One-Stop shopping for a ...short period of time and may need to do some fashionable projects. However, I hope that they also have... -
Negotiating Work and Life: How to Find the Joy
TypeBlog PostPublishedMarch 20, 2014, 3:04 p.m.... If you are miserable at home or work, your relationships will suffer in both the short run and the long...agree on before you get enmeshed in a serious relationship. #5 Thou shalt not sweat the small stuff The... -
Hot Plasmids: Fall 2024
TypeBlog PostPublishedNov. 21, 2024, 1:30 p.m....Antibody here! Anderson, Z., Zhu, H., Riedel, T., Moshinsky, D., Meijers, R. (2024). Western Blot for Anti-V5...and in ternary complexes with other proteins, establishing CRBNmidi as a promising tool for future structure-guided... -
Plasmids 101: Broad Host Range Plasmids
TypeBlog PostPublishedApril 11, 2024, 1:15 p.m.... typhimurium, S. ordonez, Serratia marcescens, Shigella spp., Vibrio cholerae, Xanthomonas campestris ...10.1111/1574-6968.12241 Keen, N. T., Tamaki, S., Kobayashi, D., & Trollinger, D. (1988). Improved broad-host-range... -
Antibiotic Resistance: An Old Solution but a New Problem
TypeBlog PostPublishedNov. 10, 2016, 3:30 p.m....difficult to treat in the future. Patients not finishing the whole course of antibiotics prescribed to ... cleanliness and personal hygiene standards. Handwashing is an essential component of preventing infection... -
Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog PostPublishedSept. 17, 2019, 12:40 p.m....vectors expressing BDNF in the hippocampus causes a shift towards antidepressant behavior in rats in the forced...neuroscience to reveal insights into the complex relationship between genes, neurons, and behavior. There ...