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Showing: 221 - 240 of 571 results

  1. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ...using CRISPR/Cas9 for genome editing, researchers simply need to express a gRNA designed to direct Cas9 ...these arrays, they did not know their functions and simply called the repeated elements “direct repeats” and...

  2. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...cell and any individual AAV particle is rare. But simply getting into the cell may not always be enough ... can optimize AAV production. We’ve listed some simple steps you can take below but you should read our...

  3. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...a white color. While CRISPRainbow allows for simultaneous visualization of sites on different chromosomes... color detection In order to label two loci simultaneously, Ma and Pederson created another sgRNA containing...

  4. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...produced? Purifying exo-AAVs is actually a bit simpler than the classical purification protocol involving...summary, exo-AAVs seem to target cell types with a similar specifically as conventionally produced AAV particles...

  6. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    Published
    Jan. 12, 2021, 2:15 p.m.
    ... you have something to ask or say. It can be as simple as “it was nice meeting you.” Include follow-up...person on LinkedIn I once helped by answering one simple question so I accepted the connection invitation...

  7. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    Published
    July 21, 2020, 1:15 p.m.
    ...dilution factor x 50 ug/ml This method is quick and simple and doesn’t require any special reagents. However...Quantifying DNA using capillary electrophoresis is similar to quantifying DNA using gel electrophoresis. Except...

  8. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...prime editing, achieving up to 3-loci MPE with similar high efficiencies, which is scalable for a higher...adeno-associated virus (AAV) and lentivirus, demonstrating simultaneous editing of genes against multiple genetic diseases...

  9. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    Published
    Dec. 20, 2018, 1:15 p.m.
    ...feasible, three separate things happened nearly simultaneously: The Wellcome Trust funded a center for mitochondrial... process of good research practice: computer simulations, in vitro research, animal models, and several...

  10. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...pocket.   5. +AMINO- by iGreenEarth AMINO is a very simple app that packs important biological information... names have a B, J, X, or Z. Some alternative, similar apps: Findings Lab Notebook Cell Culture Split...

  12. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ...strong collaborative research team motivated by similar visions and goals? We continue our conversation...and a friend. George Church runs his lab fairly similarly to his advisor Wally Gilbert: “Very interdisciplinary...

  13. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...can either express the plasmid DNA transiently (similar to bacteria) or incorporate the genetic material.... Positive selection in mammalian cells works similarly to that in bacteria and a table of the most commonly...

  14. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...Packaging using 293T cells This method is very similar to lentiviral packaging methods. The three components...constructs. Why do many γ-retroviral plasmids have similar names? The nomenclature of the plasmid backbone...

  15. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ... an advanced search option. For the former, you simply type in identifier keywords (Cas name, RefSeq ID...phylogenetic nomenclature and discover orthologs with similar properties (Makarova et al). CasPEDIA is an evolving...

  16. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...organisms. This is often cDNA, shRNA, or something similar, and is contained on the transfer plasmid. If expression...different from gamma-retrovirus, but they perform very similar functions to infiltrate the host cell and deposit...

  17. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ...management, and how does it relate to science? In the simplest of terms, a software product manager helps ensure...could code the best operations software possible. Similarly, when the developers needed to know information...

  19. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ...research purposes requires stable host cells (the simplest example would be a transformed mesophilic, for... warm weather. Psychrophilic enzymes face very similar issues when forced to express in mesophilic host...
Showing: 221 - 240 of 571 results