We narrowed to 813 results for: TIM

...pool down to plasmids expressing variants with optimal function. High-throughput DNA sequencing is then...concentration of Taq polymerase, 2) increased PCR extension time, 3) increased concentration of MgCl2, 4) increased...Wrenbeck et al for details on how nicking was optimized. How should I design my mutagenic primers? The...orientation of the BbcVI restrict site matter? Most of the time no, but there are 2 instances where it does. 1) ...saturation mutagenesis method are 1) ease of use, 2) time savings, and 3) ability to perform single site or...Stapleton, Adebola Adeniran, Keith E J Tyo, and Timothy A. Whitehead. "Plasmid-based one-pot saturation...

... to alleviate those differences, they had a hard time maintaining their friendships. I put “neutral” in...ability to remember the right stories at the right time. Like many of the posts in our Transferable Skills...address these types of harmful conflict early, because time frequently does not heal these types of wounds. ... know about those particular teams at that given time. I think about how previous iterations of those ...collaborating, and compromising. Though there may be a time and place for avoiding, competing, or accommodating...looking for a promotion, have some solid examples of times when you have used your empathy, listening skills...

...incredibly fast protocol, with only 5 hours incubation time needed for a single round of editing, and a subsequent...other advantages including shorter plasmid curing time. pCRISPomyces for editing in Streptomyces  Streptomyces...identification of a new CRISPRi tool, they also optimized the system by modulating the PAM to further tune...-Cas nucleases to produce sequence-specific antimicrobials. Nat Biotechnol 32:1146–1150 . https://doi....RJ, Mimee M, Lu TK (2014) Sequence-specific antimicrobials using efficiently delivered RNA-guided nucleases... Sommer MOA, Nielsen AT (2016) CRMAGE: CRISPR Optimized MAGE Recombineering. Sci Rep 6: . https://doi....difficile Using Phage-Delivered CRISPR-Cas3 Antimicrobials. mBio 11: . https://doi.org/10.1128/mbio.00019...

...culturist has to deal with this problem. It is estimated that mycoplasma is responsible for up to 60% of...air if the cell line remains exposed for a longer time. The aerosols are invisible to naked eyes and hence...Mycoplasmas can remain in the dry state for a longer time. When they come in contact with a nutrient source...continuous culture for at least two weeks to give time for low levels of contaminations to grow. For testing...products or reagents that have been stored for a longer time. Use fresh media as much as possible and the ones...ways to rescue the cultures. Elimination is a very time-consuming process and increases the risk of secondary...

...Tm and primer dimer estimation, and other primer characteristics. NEB Data-optimized Assembly Design (DAD...post-translational modifications and polymorphisms Codon optimization tool: To access this tool, set up an IDT account...Sequence tool.   DNA sequence manipulation tools  Sometimes DNA sequences need to be “cleaned up” or changed...Sharing your lab protocols using apps to save you time and track your experiments  Resources on Addgene.org...

... do my internship in the daytime.  Getting into the office for the first time, being introduced to all...on a time crunch and knew that if we were to restart the procedure we would not have enough time. During...I got from the La Vida Scholars tour. After some time had passed, I reached out to Amy with exciting news...

...at temperatures ranging from 30-74 °C, with an optimal growth temperature of 55 °C. For a brief comparison...Spacer Length 20 nt 22 nt Temperature Range for Optimal Activity 35 - 45 °C 50 - 70 °C Genome Editing...have increased thermostability also had a longer lifetime in human blood. To test if this was also true ...without the need to grow the organisms at lower than optimal temperatures. GeoCas9’s thermostability could also...Lucas, et al. "A thermostable Cas9 with increased lifetime in human plasma." bioRxiv (2017): 138867. Additional...

...targeted region, as shown below.  To identify optimal nickase designs, IDT scientists Mollie Schubert...and Yan next examined a situation thought to be suboptimal for HDR. At the AAVS1 locus, the two nearest ...13 nt away from the target site rather than the optimal <10 nt distance. Employing two gRNA sites that ...’s best advice: Use a PAM-out configuration Optimize your spacing D10A: nick sites separated by 37...in this post were adapted from an IDT webinar: Optimized methods to use Cas9 nickases in genome editing...

...will influence the stability of your sample over time and determine which experiments you can effectively... solution is fundamental, and it will impact the time you will be able to store your sample without having...preserving the stability of your preparation for a long time. Tris buffer controls the pH, while the EDTA chelates...stability during storage over prolonged periods of time, especially at room temperature or at 4°C. Storing...better than water in these conditions.  The next time you do a plasmid prep, plan in detail before you...

...attempting to put together such a presentation many times, I could never actually figure out what skills were... mastery. Scientists need to be doers for a long time before they master the skills they need to be successful... learners so we are all likely spending a lot of time in doer mode even late in our careers.  Managers...collaborating effectively with other teams. While they sometimes dig in and do some of the work, they must be more...others take the reins. They work alone much of the time, depending on the organization they lead. Effective...

...factors in mind at all times when making decisions, assignments and polices. Sometimes the boss has to be ...harder to manage when the measure of work is not just time spent at the desk, but it is worth it. This requires...Good relationships with clients and colleagues Sometimes this means you have to fire a good performer if...deadlines (there’s that need for flexibility, sometimes taken to an extreme by scientists) Are highly ...

...you spend a good chuck of your mental energy and time. The research project also serves as a stepping ...productivity and direction of the project? What is your timeline? A typical research project can take several years... your project will be fresh as well.” Don't underestimate the importance of choosing a solid research ...proposal before even touching a pipetteman. Take the time to generate new ideas and think about them critically...George Church (Harvard Medical School) for taking the time to speak with us about adding new scientists to ...

...advancement. Scientists do and should tell stories all the time. They provide accounts of their research struggles...interview question: Interviewer: “Tell me about a time you had a conflict at work.” Interviewee: “I did...for a paper we were publishing, but when it came time to add author details a different person in the ...great example of storytelling – you get context, time, cause, effect, and a solid conclusion. Advice for...possible — if you find yourself jumping backwards in time to explain something, rethink your structure. Develop...

...multiple timepoints during reprogramming. These library-specific identifiers act like a timestamp and help...captures the gene expression of cells at a single time point, making it difficult to investigate how early... 10-week period. Their expression decreased over time but they were not silenced.    Find CellTag libraries...tagged with different CellTag libraries at three time points during the 28 day study: prior to reprogramming...

...grafting different parts together. By combining two optimized elements of an antibody probe, scientists can ...some limitations. GFP has long protein maturation times which limits its use for imaging a protein’s entire...them hard to amplify and image for long periods of time.  Are antibody based probes a solution? To address... localization of single HA-tagged molecules over time.  The Stasevich lab also proved that HA frankenbody... a developing zebrafish embryo over an 80 minute time course.  By stitching together a stable scaffold...

...respect the time your Mentor is giving by following up on plans. Make a commitment to put time and energy... Specific, Measurable, Attainable, Relevant, and Time-bound. It is a good idea to take larger goals (e.g...and record milestones in reaching goals. The more time and thought you put into setting goals, the more... posse holds me accountable for attending and sometimes it is only the knowledge that they will be watching...

... system, each isotype is expressed at different times of the immune response and initiates different immune...occur, known as genetic drift, meaning that over time, the antibody you expect it to produce is not necessarily...antibodies are plasmid-based. Their sequences can be optimized for specificity and sensitivity, for example by...synbodies, can be produced from plasmids, saving time and money. Nanobodies and synbodies are just as ...Enzyme-linked Immunosorbent Assay). Immunoprecipitation Sometimes, instead of just detecting the presence of protein....2017.01589 Ayyar BV, Arora S, Ravi SS (2017) Optimizing antibody expression: The nuts and bolts. Methods...

...in his free time, he is pursuing a plant engineering project at BosLab. Once he optimizes plant cell culture...training prior to getting started. For example, Timothy Stiles joined BosLab last year with a computer ...experience. However, after a few months of dedicated time at BosLab, he became our bacterial transformation... we’ve developed community projects. Recently, Timothy Stiles and Mrina Nikrad have started the Open Yeast...

...saves you from having to create a new fly line every time you want to express YFG in a new tissue type (more... new fly with hedgehog under a new promoter each time a new hypothesis is tested. Controlling multiple...running with fly lines that are already available, sometimes you’ll have to make your own. In my next post,...process of generating your own fly lines. In the meantime, be sure to browse the publication references ...expression systems in Drosophila: A synthesis of time and space, Trends Genet. 20, 384–391 (2004). PubMed...

...adjust our estimates based on the journal that published the articles.  That is, we re-estimate the citations...plasmids deposited at Addgene – typically about four times as many as papers without plasmids deposited with... This is also true for other journals, so our estimates now take into account any differences in citations...deposited at Addgene still accrue on average 2 to 3 times as many citations as other papers in the same journals...