...transition to stationary phase, Segall-Shapiro et al. placed a trans-acting RepA protein under the control of...needed sequentially. When Segall-Shapiro et al. placed the genes under the control of a constitutive promoter...production titers decreased. But when the genes were placed under the control of a stabilized promoter, deoxychromoviridans...

...27.9% and homology-directed repair (HDR) taking place in 41.2% of edited cells. The results were even ...into M phase oocytes, thus allowing repair to take place before DNA replication. Indeed, they observed that...articles and commentaries) pop up on Twitter and Facebook, shared by biologists and non-biologists alike...

...quicker to pick up new languages!) Jargon has a place in science communication, but the more you use, ...of detail Again, this is an obvious one on the surface, but it’s quite easy to add in “necessary” detail...communicate. It’s okay (and encouraged) to leave blank spaces or to gloss over some aspects when needed. The ...

...talked with Voigt about this collision that’s taking place between CRISPR technology and synthetic biology,.... The Apollo 11 mission circuitry that took a spaceship to the moon was built on NOR gates. It’s a fundamental... Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 ...

...the complex, such as p300 and CBP histone acetyltransferases or CARM1 methyltransferase. This attendant...version of which will feature a simplified user interface, expanded numbers of curated molecules, including...over the years evolved from chloramphenicol acetyltransferase to luciferase, the basic assay remains widely...

...and recurrent infections along with a mild or exacerbated inflammatory response which leads to nearly 25%...inhibits a potassium channel, causing release of acetylcholine. Given its unique molecular biology and previous...pathophysiological role of glycans. Follow her on Facebook: @glycoimmunologygroup.     Michael McMurray is...

...ago. I just recently started working in the pharmaceuticals industry after earning my Master’s in 2016 ... like cliff notes, so I use these as a starting place for drafting my stories. In total, I prepare 5-6...arrangements with those you live with to have a quiet place to take your video call.  Expect technical difficulties...

...experimenter picks up a mouse from the home cage and places it into the experimental setup, the mouse becomes...Both AAV and lentiviral vectors are used for intracerebral injections. AAV particles are smaller and may...liquor of the cerebral ventricles (ICV, intracerebroventricular) to minimize tissue damage; however, the...

...indicated by exclamation marks. Error points include places where DNA is being created, such as PCR amplification...request the lab provide a new, sequence-verified replacement for the plasmid, and the full QC process is started... started over again after receiving the replacement plasmid at Addgene. QC is a team effort Addgene’s ...

...for you in one place! First, a quick refresher: prime editing is a “search and replace” genome editing..., Raguram, A., & Liu, D. R. (2019). Search-and-replace genome editing without double-strand breaks or ...

...module entirely, but instead will have to replace it with a spacer.) Each component available on the Fragmid...and selection markers. Need fewer components? A spacer can be inserted instead, allowing everything to...

...FRET pair. If you need to free up crowded optical space for multiplexing, GECO sensors are available in ... violet light and (2) exposure to increased intracellular calcium. It has been applied to mark active ...Whether you’re studying the brain, muscles, or intracellular landscape, remember: Keep Ca+2lm and Ca+2rry...

...formation of disulfide bridges, glycosylation and acetylation to create functional, stable proteins. Protein...range of proteins including antibodies and pharmaceuticals, specifically interleukins. Yeast expression...biochemistry. When using S. cerevisiae, researchers often place recombinant proteins under the control of the galactose...

...proteins (Wrapp et al., 2020), which sit on the surface of coronaviruses and enable the virus to enter ...in the molecular crevices proteins on the cell surface. Figure 1: Comparison of common antibody,... is tricky because they have fewer hydrophilic surfaces, require detergents or lipids to remain folded...

...definitive, but visual identification is a good place to start before taking on more time consuming and...fitness cost yet. However, many fluorescent proteins face similar limitations, and many labs are already using...Figure 4). They created ShadowR by modifying the surface of the chromoprotein Ultramarine with hydrophilic...

...but it actually requires more technical skill to place the needle in the correct vein without collapsing...injections are given in the peritoneal cavity, or the space surrounding organs in the abdomen. They are a popular...cerebral spinal fluid. The injection site is the space in and around the vertebrae, so hitting the correct...

...Korte et al., 2021) for all disposable items and surfaces that come into contact with the rAAV and apply...centrifugation (e.g., iodixanol) using a high-speed ultracentrifuge. During the spin, the virions will form a band...ddPCRAAV Purification by Iodixanol Gradient UltracentrifugationAAV Production in HEK293 Cells...

...technology that allows precise modifications of DNA (replacements, small insertions, and deletions) without introducing.... The pegRNA consists of a target-complementary spacer, a scaffold sequence, and an extension sequence..., Raguram, A., & Liu, D. R. (2019). Search-and-replace genome editing without double-strand breaks or ...

...the genome and located immediately 5’ to a protospacer adjacent motif (PAM) sequence. Co-delivery of wild-type...produces the phenotype you screened for in the first place. With the proper experimental design and validation...

... who need to overexpress and purify proteins can face any number of technical challenges depending on ...protease. A TEV protease cleavage site is frequently placed between two tags being used for two rounds of purification...purification, with the cleavage reaction taking place between column runs. The TEV protease itself, with...