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Addgene

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We narrowed to 815 results for: ache

Showing: 221 - 240 of 815 results

  1. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...foreign dsRNA activates the innate immune response machinery of the host. This pathway has been clinically... response is activated if and when dsRNA levels reach a given threshold within the cell, as is often the...viral infection. Endogenously produced dsRNA rarely reach this threshold and thus do not typically activate...and H2) handle most of these intermediates, with each one acting on specific substrates and in distinct...cycles. When RNA:DNA hybrids levels are allowed to reach very high or low levels, possibly due to dysregulation...

  2. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...came on March 9, 2005. It took almost six years to reach our first major repository milestone — 10,000 plasmids...did this the old-fashioned way: through physical outreach, visiting organizations, knocking on doors, and...approvals. In 2013 we were thrilled to receive the Massachusetts Nonprofit Network Excellence Award for our electronic...   Expanding our work In March 2015, Addgene reached the 500,000 plasmids distributed milestone! We ... Rachel Leeson...

  3. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...Scientists are currently doing this using two approaches: single-cell RNA-sequencing to identify neuronal...neuronal class and deep sequencing of neurons from each class to fully characterize the transcriptome. The... 2019. For more information about the aims and approach of CeNGEN, check out their publication.   3’UTRome... this tool by splitting cGAL4 in two and binding each half to a gp41-1-N-intein. Split inteins are protein...for the creation of CRISPR targeting vectors that each contains a guide RNA and repair template of your...

  4. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ... a good idea to prepare 1-2 unique questions for each interviewer by looking at their LinkedIn profiles.... I’d also use this notebook to take notes after each interview.   Tailor your 1-hour presentation to ... numerous people during your industry interview. Each interview is an opportunity to learn something and...clarification on the dress code for the interview. Greet each interviewer and then thank them once the interview...question. Maybe you ask for questions after completing each section of your talk. Some video conferencing software...

  5. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ...number of papers with your search term published each year, which is a nice way to see growth or decline... in order to turn Twitter into a paper-finding-machine. There are four general types of accounts you can...Scholar analyzes publications and figures using machine learning to extract important features such as ...the number of papers with your key term published each year is displayed with your search results and can...your feed. Feeds are organized by papers published each day, week, or month. A measure of the number of ...

  6. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    ... the target protein, so they do not compete with each other. This antibody is also conjugated to another...reflects the concentration of your target protein in each well. The reaction is stopped by the addition of...reaction, turns the solution yellow. The absorbance of each well, which is measured using a microplate reader..., so if you max out your standard curve and it reaches an asymptote, you won’t be able to accurately calculate...portion or the OD of your experimental samples has reached 4.0, you can resolve this issue when you repeat...

  7. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    Published
    Aug. 1, 2023, 1:15 p.m.
    ...thought otherwise! They suspected a data-driven approach might allow for an increase in the complexity....to sets of up to 6-8 overhangs, the data-driven approach was needed to massively expand complexity. The...search window for a site—or sites—that must be used. Each of the tools works with either 3-base or 4-base ... 10-piece circular assembly, with the 52-piece approach yielding approximately ~800-fold fewer plaques...  Rachel Leeson...

  8. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...that are easily distinguishable from each other. In a direct approach, that is simple - but what about when...a mix of different isotypes and subclasses. They each have their own advantages. Since monoclonal antibodies...use an indirect (primary and secondary antibody) approach?  Staining for multiple targets When staining ...plan primary/secondary antibody pairing such that each target has a unique species or isotype that can ...

  9. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...When possible, the PE3b/PE5b approach is recommended over the PE3/PE5 approach. Figure 7: Nicking...with BioRender.com. PE3b and PE5b: In this approach, the nicking sgRNA is designed to bind only after...BioRender.com. MMR inhibitors PE4 and PE5: These approaches inhibit MMR to improve editing efficiency, but...BioRender.com. That’s all our tips for today! Each tip includes a citation to the paper it was drawn...

  10. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...insertions or deletions in the DNA sequence. This same approach is effective to target short ncRNAs, such as microRNAs...A schematic illustration of CRISPR/Cas9-based approaches to study ncRNAs. (A) Transcription start site... sgRNA to temporarily block the transcription machinery of a target gene or DNA that codes for ncRNA. ...to enhance or upregulate ncRNA expression. This approach is useful for lncRNAs because they regulate nearby...science.1225829  Hazan J, Bester AC. CRISPR-Based Approaches for the High-Throughput Characterization of Long...

  11. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    Published
    Jan. 21, 2015, 1:30 p.m.
    ...supervisor, or do you have to work with difficult people? Each time I ask this question at my workshops, I get ...trouble making decisions. In my work as a thesis coach for graduate students, I heard about supervisors...work hours. While specific strategies to deal with each of these personality types is beyond the scope of...and if you cannot resolve a problem on your own, reach out for help so you can get the support you need...Find plasmids to Expedite Your Experiments and Achieve Your Research Goals    ...

  12. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ... it has been challenging to introduce CRISPRi machinery into diverse bacteria species. Existing protocols... disease-associated. Mobile-CRISPRi: a modular approach for CRISPRi transfer Mobile-CRISPRi offers two...bacteria with a chromosomal copy of the RP4 transfer machinery mobilize these plasmids for transfer to recipient...contain a chromosomal copy of the RP4 transfer machinery, which mobilizes the Tn7 plasmids, and lack the...wild-type bacteria and the relative frequency of each of the 40 library strains was measured after 6 and...

  13. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...expression vector pSb-Init, which attaches a Myc-tag and a His6-tag to the end of each sybody candidate. These ...have gotten a lot of press for being a promising approach for treatment or prophylaxis of COVID-19. Researchers...nanobody structures (concave, loop, and convex). Each of these 3 mRNA libraries have a concave, loop, ... for in-vitro translation of the mRNA libraries. Each mRNA molecule in the library doesn’t encode a stop...

  14. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ...proportionally to the amount of product produced each cycle.  To determine the concentration of the starting...reaction sample into approximately 20,000 droplets. Each droplet contains the material required for PCR amplification... amplification. Following the PCR reactions each droplet is analyzed by a droplet reader, which measures...measures the fluorescence amplitude of each droplet. The fraction of fluorescent PCR-positive droplets is ... primer pairs that align to the target region of each template are used in one reaction. Multiplex PCR...

  15. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...efficiency barrier in tomatoes" by combining several approaches (Vu et al., 2024). Their optimized strategy uses...CC-BY-NC-ND 4.0 International license. With this approach, the team was able to drive prime editing in both...inherited the genomic edit but not the editing machinery. Together, these advances have the potential to...developed a cost-effective expression and labeling approach using these constructs in HEK 293 cells (Smith...) biosensors (Janicot and Maziarz et al., 2024). Each plasmid expresses all the components of the biosensor...

  16. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...common types of degrons and some of the nuances to each one.   SMASh Small molecule-assisted shutoff (SMASh...self-cleave and the protease and degron remain attached to the tagged protein. In this event, the degron... tag, and the endogenous cellular degradation machinery to function. The dTAG is the smallest tag reviewed...functions – there are antibodies to it and ways to achieve fluorescence with it. AID The auxin-inducible degron...441. doi: 10.1038/s41589-018-0021-8 Holland AJ, Fachinetti D, Han JS, Cleveland DW. Inducible, reversible...

  17. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...with many of the same drawbacks to each reagent type, so this approach is not suitable for all antibodies...and outlined the considerations to be made during each step. Now that you know the basic principles of ...the accessibility of your target epitope(s). To achieve these goals, researchers commonly use one of two...detergents, such as Triton X-100, Tween-20, or saponin. Each detergent has different chemical properties that...

  18. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...limited in the level of resolution that they can achieve. This means that regardless of how powerful of ..., you can’t resolve two points within ~250 nm of each other. For reference, individual presynaptic terminals...sample (i.e. cultured cells or tissues).  Step 2: attach anchors to your sample that will link biomolecules...evenly in all directions, aka isotropically. But achieving isotropic expansion depends on how well the gel...especially when paired with complementary experimental approaches. Since it arrived on the scene, ExM has helped...

  19. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...-associated genetic dependencies associated with each ancestry group. Since about 90% of the cell lines...their data.     Fig. 1: Genomic fraction for each of five major ancestry groups for 994 of the Cancer...RNA that bind to a cell’s DNA, telling the Cas machinery where to cut.      Fig. 2: Heatmap indicating...): 250-252. Carlson J, Henn BM, Al-Hindi DR, Ramachandran S. Counter the weaponization of genetics research...  Rachel Leeson...

  20. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ... to be able to cross the blood-brain barrier to reach the brain. Delivering the CREATE AAV library via...jumble of fluorescent neurons. A simple but flawed approach to multi-color labeling is to inject a mix of ...was derived from figure 3b in Chan et al.   To achieve the full diversity of colors AND sparse labeling...co-injected. The dose of this AAV can be adjusted to achieve sparse levels of labeling, without loss of color...virus, but the desired sparsity of labeling is achieved with lose doses of inducer virus. Additionally...
Showing: 221 - 240 of 815 results