We narrowed to 282 results for: c rel

...signaling dynamics are influenced by the relatively long life-cycle of a protein from expression to degradation...the mitochondrial targeting sequence of cytochrome c oxidase subunit VIII, so that they can be imported...pCoofy series of plasmids contain a variety of N- and C-terminal tags (including His, S-tag, OneStrep, CBP...N-terminal tag, coding sequence of the gene of interest, C-terminal tag, plant terminator, and plant resistance...compartment of action via sequestration. The strategy relies on several observations: Rapamycin is a membrane-permeable...physiological setting. Genetic techniques typically rely on overexpression or knock-down, where signaling...or small molecules and exceed a physiologically relevant exposure in intensity and/or duration. Both of...

...https://doi.org/10.1038/s41598-020-78827-9  Breen, C. J., Raverdeau, M., & Voorheis, H. P. (2016). Development...Muthusamy, A. K., Liang, Y., Brown, T. A., Lemon, W. C., Patel, R., Lu, R., Macklin, J. J., Keller, P. J....60 minutes of illumination, highlighting its reliability for long-term experiments (Salari et al., 2019...unconjugated dye molecules are critical for accurate and reliable imaging and assay results (Hayashi-Takanaka et...

...allow the digestion to take place for an hour at 37°C. This should be enough time to completely digest the...time requirements. FastCloning is a simpler yet reliable cloning technique that was developed by Li, et...

...of our most popular mammalian expression vectors, c-Flag pcDNA3, comes from the pcDNA series. It has the...pDEST-HisMBP). After purification the protein can be released from the resin and used for various binding or...

...technologies nanopore-based DNA sequencing machines are relatively small meaning that DNA sequencing could move ...1975): 503-517. PubMed PMID: 1195397. 2. Chang, Judy C., and Yuet Wai Kan. "A sensitive new prenatal test...next-generation sequencing techniques do not typically rely on Sanger sequencing but instead use methods like...

...Lindenbaum P, Martin-Fontaine M, Broucque F, Robin C, Hebben M, Merten O-W, Blouin V, François A, Redon...preparation approaches described previously require a preliminary double stranding step. The double stranding step...preparations and showed that VGS can quickly and reliably confirm the identity of AAV genomes and detect...the Addgene blog Read all of our viral vectors related blog posts Read our blog post on AAV titers Browse...

... I as this enzyme recognizes the DNA sequence 5´-(C/T)CCTT-3' and digests double stranded DNA at this ...post-PCR procedures. Sounds easy right? The technique relies on the basic ability of complementary basepairs...covalent bond thus joining the two DNA strands and releasing topoisomerase (2).  These days commercially available...extra salt to your reaction: Topoisomerase I is released from the vector when the PCR product and vector...

...Gelsinger, D. R., Vo, P. L. H., Klompe, S. E., Ronda, C., Wang, H. H., & Sternberg, S. H. (2024). Bacterial...genomes (Peters et al., 2017). Type I CAST systems rely on the Cascade complex minus its nuclease component... found to be particularly useful. But they were barely effective at editing eukaryotic cells — at least...

...light. Previous iterations of FLiPs only used N- and C-terminal dronpa fusions. With these various designs... produce two biosensors that could quickly and reliably detect HMOs. Fatima’s first design is a whole ...enzyme breaks down the HMO of interest, the lactose released activates expression of GFP from the lac operon...

...depositing can be grown using standard conditions (37 °C, LB, antibiotics, etc.) and stored as glycerol stocks...drop-down menu to indicate whether the tag is on the N or C terminus and whether it was present in the backbone...) after three years on hold, Addgene staff may release the hold unless otherwise requested. Distribute...assembled or theoretical — it does not have to be entirely verified by sequencing. Upload any support files...easier for other scientists to find information related to your plasmid. It is optional to include the ...and alternative name boxes as needed. Use the "Relevant mutations/deletions" box to describe any mutations...

...arrays (e.g. for spacers A, B, and C, you could obtain arrays A, B, C, AB, AC, BC, and ABC). Nielsen Lab...specific sites that allow them to be cleaved and released. These constructs tend to be smaller than constructs... If Csy4 is not expressed, the gRNAs cannot be released, adding temporal and/or spatial control to the...recognize the tRNA sequences, cleave them, and release the gRNAs. PTGs are assembled into a wt Cas9-containing...editing of six different genes. This system doesn’t rely on any additional or host-dependent factors and ...

...on designing and using RNA scaffolds: Delebecque, C. J. et al. Designing and using RNA scaffolds to assemble...rational, straightforward design. Since the technique relies on base pairing, it has promise to be host-independent...

...this may be due to different lipid compositions of C. elegans membranes, which might beneficially influence... of ApoB-LPs has been shown to have a strong correlation with cardiovascular disease and indirect measurement...that the system is active in E. coli and closely related species, is amenable to use with other bEBP/UAS...and the system may not be adaptable to distantly related species from E. coli. This tool should serve as...

... untagged, cleavage sites (thrombin and TEV), and C-terminal tags (StrepII, 3xFLAG, 6xHIS, MBP, Myc). ...For the many other COVID-19 related plasmids in our collection, we’ve relied on Sanger sequencing as a ...

... Cas9 for easy genome engineering in species like C. elegans and Drosophila. Light inducible promoters...heat shock. In the case of Hsp70, the heat shock releases heat shock factor 1 (HSF-1), which subsequently...cells and typically only in the same or closely related species from which they were derived. Similarly...

...long been extremely useful in the lab – they are relatively small and easy to work with, making them the ...10.1016/j.cell.2013.04.025 Zhang X, Liang P, Ding C, Zhang Z, Zhou J, Xie X, Huang R, Sun Y, Sun H, Zhang...the day, March 16, 2014, that I got my first preliminary results from a CRISPR genome editing experiment...

... tracrRNA and Cas9, bind to these transcripts and C) cleave them leaving mature crRNAs bound to the Cas9...use a non-specific endonuclease (Cas9 or closely related Cpf1) to cut the genome and a small RNA (gRNA) ...

...to produce and distribute virus. Addgene is a 501(c)3 nonprofit and our plasmid service was started based... Addgene’s Legal Team acquired permission from relevant plasmid depositors and their institutions to distribute...

...and References References Wang, I. X., Grunseich, C., Fox, J., et al., Human proteins that interact with...bidirectional transcription. In an uninfected cell, dsRNA rarely circulates, but upon viral infection, this nucleic...barring a loss of mitochondrial integrity that would release them into the cytosol. Fig. 2: Endogenous...upon viral infection. Endogenously produced dsRNA rarely reach this threshold and thus do not typically ...

...Central PMCID: PMC3785459. 2. Krashes MJ, Koda S, Ye C, Rogan SC, Adams AC, Cusher DS, Maratos-Flier E, Roth...CRISPR tools, though we do provide a few non-CRISPR related viral preps. Cas9 is the headliner of most CRISPR...mCherry Our current AAV inventory consists almost entirely of viruses expressing DREADDs from Bryan Roth’...