We narrowed to 284 results for: cki
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TypeBlog PostPublishedJan. 19, 2023, 2:15 p.m....involve taking the Myc1-9E10 variable regions and sticking them onto different scaffolds. But over the years...
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10 Basic tips for mammalian cell culture
TypeBlog PostPublishedJuly 12, 2018, 1:09 p.m....wiped with 70% ethanol immediately. Finally, avoid sticking pipettes into solution bottles directly, rather... -
Degrading DNA with Cascade-Cas3
TypeBlog PostPublishedFeb. 11, 2025, 2:15 p.m....carried out much like the name implies — after nicking, Cas3 reels in the non-target strand of DNA much... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog PostPublishedFeb. 23, 2016, 3:30 p.m....sets of microhomologies (5’ and 3’) to anneal, knocking the GFP-Puro cassette into the locus (see figure... -
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs
TypeBlog PostPublishedJune 25, 2024, 1:15 p.m....prediction models. In addition, we provide options for nicking gRNAs (used for PE3 and PE5 prime editing strategies... -
Truncated gRNAs for Regulating Gene Expression
TypeBlog PostPublishedJan. 10, 2017, 3:37 p.m....PMC4747789. 6. Platt, Randall J., et al. "CRISPR-Cas9 knockin mice for genome editing and cancer modeling." Cell... -
Which Fluorescent Protein Should I Use?
TypeBlog PostPublishedMay 20, 2014, 2:06 p.m....the FP (fused to the protein of interest), thus tracking the localization of that biomolecule inside living... -
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection
TypeBlog PostPublishedDec. 12, 2013, 3:35 p.m....Who owns computer-programming languages? That's trickier. Sometimes it's the developers. But, it turns ... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog PostPublishedAug. 27, 2020, 1:15 p.m....sequences, the amino acid sequences RSIAT and RPACKIPNDLKQKVMNH have been used successfully (Kerppola, 2006... -
Capturing a Moment with Marc Zimmer’s “The State of Science”
TypeBlog PostPublishedJuly 30, 2020, 1:15 p.m....likely range from mildly surprising to genuinely shocking. Mainstream media outlets have addressed CRISPR... -
DNA Purification Without a Kit
TypeBlog PostPublishedFeb. 11, 2020, 2:15 p.m.... bonds with water molecules and coaxes it into sticking to the hydrophobic glass filter. Each glass filter... -
A Guide to Starting Your Own Journal Club
TypeBlog PostPublishedFeb. 6, 2020, 3 p.m....time to stay current or enjoy that discussion. Luckily, one needn't worry if they choose to move away ... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog PostPublishedFeb. 27, 2018, 2:04 p.m....available nanobodies from Pleiner et al. Before picking a secondary nanobody, it’s important to review ... -
Lentiviral Vector Uses and Overview
TypeBlog PostPublishedMay 19, 2016, 2:30 p.m....In-vivo RGB marking and multicolour single-cell tracking in the adult brain. Gomez-Nicola D, Riecken K,... -
Transferable Skills Guide: Identifying Your Transferable Skills
TypeBlog PostPublishedJan. 11, 2018, 3:29 p.m....retrieved your lucky socks, persevered through a nerve wracking commute complete with a disabled train, and now... -
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog PostPublishedDec. 2, 2014, 7:23 p.m.... molecules even enables us to skip the step of knocking-in. In 2013, we showed that isolation of a single... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog PostPublishedMay 15, 2014, 3:33 p.m....Because of its stability, GFP can be used in lineage tracking capacities in cell fate studies. It can also be... -
Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog PostPublishedSept. 17, 2019, 12:40 p.m.... circular corridor apparatus using a camera or tracking software. Locomotion is a highly complex process... -
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More
TypeBlog PostPublishedAug. 13, 2019, 12:58 p.m.... target mRNA inhibit its translation either by blocking initiation or splicing. (RNAi, in contrast, engages... -
Save Time with Transient Plant Leaf Transformations
TypeBlog PostPublishedJuly 25, 2019, 12:03 p.m.... your tissue. Run a control with Agrobacterium lacking the GUS gene since plants can have endogenous GUS-like...