We narrowed to 245 results for: construct
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TypeBlog PostPublishedMay 13, 2025, 1:15 p.m....plasmid available at Addgene: psPAX2-D64V. This construct has the D64V mutation, a single amino acid change...
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Harnessing Bacterial Toxins for Allelic Exchange
TypeBlog PostPublishedAug. 15, 2019, 12:30 p.m....Valentovich LN. "DNA tuner: a computer program for the construction of polylinker sequences of molecular vectors... -
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection
TypeBlog PostPublishedApril 9, 2019, 1:13 p.m.... design, it is advisable to generate multiple constructs with different insertions, since it is difficult... -
Using Phosphoserine to Study Protein Phosphorylation
TypeBlog PostPublishedJune 23, 2016, 2:30 p.m....Journal. Resources on the Addgene Blog Verify Your Constructs with Colony PCR Read about Tags for Protein Purification... -
Evolution of Lab Techniques
TypeBlog PostPublishedJune 21, 2016, 2:30 p.m..... Kulkarni. "Overview: engineering transgenic constructs and mice." Current Protocols in Cell Biology ... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog PostPublishedMay 10, 2016, 2:30 p.m....increased activation of HIV promoter-luciferase constructs. In multiple HIV-1 latent T cell lines, as well... -
Technique: Probe Phage Genomes for Host Binding Proteins
TypeBlog PostPublishedSept. 29, 2016, 2:30 p.m....Schmitz, Jonathan E., et al. "Rapid DNA library construction for functional genomic and metagenomic screening... -
Plasmids 101: Repressible Promoters
TypeBlog PostPublishedDec. 20, 2022, 2:15 p.m....stage-specific promoter and design a UAS reporter construct, the reporter will only be expressed where and... -
Viral Vectors 101: Optogenetic Tools
TypeBlog PostPublishedJune 27, 2023, 1:15 p.m....to know (and understand!) the hardware types, construction, and testing used for delivering your light ... -
Viral Vectors 101: AAV Variables That Matter
TypeBlog PostPublishedJune 1, 2023, 1:15 p.m....expression in your experiment. Some inducible constructs can exhibit leaky expression in the absence of... -
Quick Guide to All Things Lentivirus
TypeBlog PostPublishedMarch 21, 2017, 2:30 p.m....the different vectors used, lentiviral vector constructs have proven very useful due to their ability ... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog PostPublishedOct. 17, 2019, 12:28 p.m.... the recombination of the double-floxed opsin construct, thereby enabling its expression under a strong... -
Selecting Your Plasmid Purification Kit
TypeBlog PostPublishedMay 27, 2025, 1:15 p.m....cell model, or overall throughput needed. Large constructs – Large plasmids, cosmids, or BACs, can be tricky... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog PostPublishedJuly 19, 2016, 2:30 p.m....experiments include producing a positive control construct where the donor and the acceptor fluorophore binding... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog PostPublishedDec. 17, 2020, 1:30 p.m....SapTrap Builder greatly simplifies design of these constructs, allowing novice researchers to quickly and easily... -
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins
TypeBlog PostPublishedApril 25, 2017, 2:30 p.m....enough molecules have been analyzed to allow for construction of an image, providing detailed protein localization...fluorophore being imaged multiple times in order to reconstruct an image. The best PA-FPs used for super-resolution... -
Optogenetics + CRISPR, Using Light to Control Genome Editing
TypeBlog PostPublishedSept. 3, 2020, 12:15 p.m....using Minicircle DNA vectors encoding the FAST constructs. This proof-of-concept model opens intriguing... -
Light Sheet Fluorescence Microscopy
TypeBlog PostPublishedJuly 16, 2015, 2:30 p.m....Using this method, these authors were able to reconstruct a cleared guinea pig cochlea in 3D. The basic...configuration of the instrument used in this reconstruction lay the foundation for all subsequent versions...sheet microscopy with sequential multiple-view reconstruction (5). In order to image large tissue such as...resolution (8,9). Thus, LSFM is ideal for 3D reconstruction of clear specimens and for in vivo imaging ...development of vertebrate embryos. The first 3D reconstructions of clear embryos were obtained by using optical... -
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More
TypeBlog PostPublishedAug. 13, 2019, 12:58 p.m....cells from 694 embryos over multiple stages and reconstructed the patterns of gene activity and cell divisions...30944477. Farrell, Jeffrey A., et al. "Single-cell reconstruction of developmental trajectories during zebrafish...PMID: 29853675. Keller, Philipp J., et al. "Reconstruction of zebrafish early embryonic development by... -
Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog PostPublishedJuly 23, 2015, 2:30 p.m.... are not yet fully understood. We attempt to deconstruct and understand this complexity using existing...