We narrowed to 247 results for: construct

...produced 90% accurate integration of multiplexed constructs into target genomes and a 10-fold improvement...

...comments on your manuscript is a form of (hopefully constructive) conflict. As you perform the experiments to...

...plasmid available at Addgene: psPAX2-D64V. This construct has the D64V mutation, a single amino acid change...

...Valentovich LN. "DNA tuner: a computer program for the construction of polylinker sequences of molecular vectors...

... design, it is advisable to generate multiple constructs with different insertions, since it is difficult...

...Journal. Resources on the Addgene Blog Verify Your Constructs with Colony PCR Read about Tags for Protein Purification...

.... Kulkarni. "Overview: engineering transgenic constructs and mice." Current Protocols in Cell Biology ...

...increased activation of HIV promoter-luciferase constructs. In multiple HIV-1 latent T cell lines, as well...

...stage-specific promoter and design a UAS reporter construct, the reporter will only be expressed where and...

...Schmitz, Jonathan E., et al. "Rapid DNA library construction for functional genomic and metagenomic screening...

...to know (and understand!) the hardware types, construction, and testing used for delivering your light ...

...expression in your experiment. Some inducible constructs can exhibit leaky expression in the absence of...

...the different vectors used, lentiviral vector constructs have proven very useful due to their ability ...

... the recombination of the double-floxed opsin construct, thereby enabling its expression under a strong...

...SapTrap Builder greatly simplifies design of these constructs, allowing novice researchers to quickly and easily...

...cell model, or overall throughput needed. Large constructs – Large plasmids, cosmids, or BACs, can be tricky...

...experiments include producing a positive control construct where the donor and the acceptor fluorophore binding...

...enough molecules have been analyzed to allow for construction of an image, providing detailed protein localization...fluorophore being imaged multiple times in order to reconstruct an image. The best PA-FPs used for super-resolution...

...using Minicircle DNA vectors encoding the FAST constructs. This proof-of-concept model opens intriguing...

...Using this method, these authors were able to reconstruct a cleared guinea pig cochlea in 3D. The basic...configuration of the instrument used in this reconstruction lay the foundation for all subsequent versions...sheet microscopy with sequential multiple-view reconstruction (5). In order to image large tissue such as...resolution (8,9).  Thus, LSFM is ideal for 3D reconstruction of clear specimens and for in vivo imaging ...development of vertebrate embryos.  The first 3D reconstructions of clear embryos were obtained by using optical...