...Schmitz, Jonathan E., et al. "Rapid DNA library construction for functional genomic and metagenomic screening...

...the different vectors used, lentiviral vector constructs have proven very useful due to their ability ...

...to know (and understand!) the hardware types, construction, and testing used for delivering your light ...

...expression in your experiment. Some inducible constructs can exhibit leaky expression in the absence of...

... the recombination of the double-floxed opsin construct, thereby enabling its expression under a strong...

...cell model, or overall throughput needed. Large constructs – Large plasmids, cosmids, or BACs, can be tricky...

...SapTrap Builder greatly simplifies design of these constructs, allowing novice researchers to quickly and easily...

...experiments include producing a positive control construct where the donor and the acceptor fluorophore binding...

...enough molecules have been analyzed to allow for construction of an image, providing detailed protein localization...fluorophore being imaged multiple times in order to reconstruct an image. The best PA-FPs used for super-resolution...

...using Minicircle DNA vectors encoding the FAST constructs. This proof-of-concept model opens intriguing...

...Using this method, these authors were able to reconstruct a cleared guinea pig cochlea in 3D. The basic...configuration of the instrument used in this reconstruction lay the foundation for all subsequent versions...sheet microscopy with sequential multiple-view reconstruction (5). In order to image large tissue such as...resolution (8,9).  Thus, LSFM is ideal for 3D reconstruction of clear specimens and for in vivo imaging ...development of vertebrate embryos.  The first 3D reconstructions of clear embryos were obtained by using optical...

...cells from 694 embryos over multiple stages and reconstructed the patterns of gene activity and cell divisions...30944477. Farrell, Jeffrey A., et al. "Single-cell reconstruction of developmental trajectories during zebrafish...PMID: 29853675. Keller, Philipp J., et al. "Reconstruction of zebrafish early embryonic development by...

... are not yet fully understood. We attempt to deconstruct and understand this complexity using existing...

...chromosomes via a cut and paste mechanism. Scientists reconstructed a transposase system based on the naturally ...

...Fourier-shell correlation curve (D), and the reconstructed 3D map with 3.1 Å global resolution (E). Figure...

...antibodies fall into two groups. Seven are reconstructions of the original hybridomas as new reproducible...

...Emission Depletion (STED), and Stochastic Optical Reconstruction Microscopy (STORM). These dyes have specific...

...editors by expression optimization and ancestral reconstruction. Nature Biotechnology, 36(9), 843–846. https...