We narrowed to 513 results for: gats

...possible pitfalls in your strategy and how can you mitigate them? If you were to write a grant for this project...project along the way. Advice from principal investigators Tom Ellis highlights the importance of keeping...

... focus on how to choose an advisor/principal investigator (PI) that will also serve as a good mentor. ...use publication records, university websites, ResearchGate and LinkedIn to find past lab members. Most ...

...partial loss of an ITR during routine plasmid propagation, with complete ITR loss abolishing AAV packaging...packaging. To prevent ITR loss, it’s best to propagate AAV constructs in recombination-deficient bacterial...

...advancing our knowledge in areas such as bacterial conjugation and recombination, replication and topology, ...Central PMCID: PMC1209583. Watanabe, Tsutomu, Chizuko Ogata, and Sachiko Sato. “EPISOME-MEDIATED TRANSFER OF...

...funded a center for mitochondrial research to investigate the scientific possibility, safety, and efficacy...efficacy of the mtDNA transfer options being investigated; A UK regulatory body tasked two separate non-governmental...

...Transcription factors (TFs) act as the gate keepers for gene expression, turning transcription on and...individual plasmids from the library to further investigate specific genes and TF isoforms. Due to the size...

...followed these experiments with more detailed investigations into the release of DNA post-cut. Using substrate...Postdoctoral Researcher in Jacob Corn’s lab investigating DNA repair pathways.     Additonal Corn Lab ...

...kinase. This hybrid construct senses light, and an obligate chromophore allows the system to respond to various...photosensor senses various light inputs, and an obligate chromophore allows the system to respond. In far-red...

...Chromosome Organization. These aren’t the only investigators in the chromatin field who have deposited with...small, sensitive immunohistochemical reagents. Yamagata M, Sanes JR Proc Natl Acad Sci U S A. 2018 Feb...

...editing speed dictates which phage variants can propagate faster than the rate of dilution (passage from...off-target DNA and off-target RNA activity. To mitigate this drawback, the lab introduced an additional...

... into modules that students and teachers can investigate together. Teachers decide which BioBuilder lessons...means to rapidly prototype DNA circuits and logic gates. This BioBuilder-Caltech collaboration has resulted...

...our SnapGene powered plasmid maps far easier to navigate. These practical and effective changes will give...restriction enzymes that are commonly used for Golden Gate cloning or for CRISPR gRNA cloning. With this more...

...i) brightness, (ii) photostability and (iii) aggregation tendency. Brightness Brightness refers to the...information about properties like photostability or aggregation tendency, our choice would be mKOk-2A-mTq2; it...

...experience of our website, the different website navigation paths, the organization and completeness of our...all Addgenies involved). The new homepage and navigational elements help users who prefer to browse and...

... blog post, we covered how to use positive and negative selection in plasmid cloning.  But oftentimes...experiment, you’ll want the proper positive and negative controls for your PCR.  Find the details and learn...

...mutations and truncations that can make vector propagation and sequencing a headache. How do I maintain ...integrity and prevent unwanted mutations?  During propagation, mutants rapidly replicate in a growth culture...

...extremely tricky to find: each of the factors can negatively impact both translation and fidelity of translation...possibilities for researchers, from selective protein ligation through introducing ncAAs with protein modifications...

...partition is individually assessed for a positive or negative (digital) signal at the end of the reaction (Figure...contain any target DNA at the onset of PCR are negative, whereas those that did are positive. Since there...

... methylase adds a methyl group to the adenine of GATC stretches of DNA Dcm methylase adds a methyl group...and will therefore have methylated adenines in any GATC sequences found in the plasmid. When the PCR products...

...each one requires different elements for vector propagation. This post, along with a future companion post...yeast plasmid) allows for 50+ copies to stably propagate per cell. The copy number of these vectors can...