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We narrowed to 411 results for: genome

Showing: 221 - 240 of 411 results

  1. Grad School Advice Part 1: Picking a Lab and a Project

    Type
    Blog Post
    Published
    Sept. 1, 2016, 2:30 p.m.
    ... Ph.D candidate in the Program in Genetics and Genomics at Harvard Medical School. He studies developmental... a graduate of the PhD program in Genetics and Genomics at Harvard Medical School, and is currently a ...

  2. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    Published
    May 3, 2018, 1:48 p.m.
    ...enzymes can knock down RNA without altering the genome, but Cas13s have higher on-target specificity. ... two guide RNAs to remove a given exon from the genome, and showed success in models of muscular dystrophy...CRISPR systems, only time will tell how large the genome and transcriptome engineering toolbox will be. ...characterized orthologs from “Anaerobic digester metagenome” (AdmCas13d) and Ruminococcus flavefaciens (nicknamed...

  3. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    Published
    Feb. 16, 2023, 7:05 p.m.
    ...at a specific genomic site, or randomly integrated (once or many times) into the genome. Make sure to ...lentivirus and retrovirus can package cDNAs for genomic delivery and have many therapeutic advantages, ...

  4. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ...transcription-activator-like effectors (TALEs) as anchors for genome engineering applications was still very new. Now...role of specific epigenetic modifications in the genome,” Zhang said in MIT’s press release. In an accompanying...

  5. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...Interested in more genome engineering technologies? Browse others on Addgene's Genome Engineering Guide...genes, reporters, or other markers into a target genome. They did this by selecting the important elements...fusion proteins with a variety of genes across the genome. Although this tool was published in 2011, it was...scientists to use CRISPR-Cas technology to perform genome-wide knockout screens. These vectors and sgRNA ...our blog post,Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening. Also, check out our ...plasmid that was used by Chen and colleagues for genome editing inXenopus tropicalis. (Guo et al., Development...Development. 2014..) A new, higher specificity genome editing system that combines TALENs and CRISPRS. Developed...

  6. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...The Rare Genomics Institute BeHEARD (Helping Empower and Accelerate Research Discoveries) Challenge provides... year is our fourth year working with the Rare Genomics Institute by providing plasmid-based tools for...Additional Resources Find out more about the Rare Genomics Institute and this year's BeHeard Award! ...

  7. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...component that physically integrates into the host genome, the virus is replication incompetent, meaning ...humans and does not always integrate into the host genome as part of the viral infection process (though ...AAV in that it does not integrate into the host’s genome. However, some adenovirus strains are known to ...

  8. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...especially useful within both the synthetic biology and genome engineering fields. We’ll walk you through how ...website or Engler & Marillonet. Golden Gate and genome engineering In early 2011, the Bogdanove and Voytas...described a new Golden Gate-based technology for genome editing which allowed for the ordered assembly ...

  9. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...the worm genome and make additional mutations in our worms.  We are on the lookout for genome modifications...molecular biology are based on the standard worm genome. While C. elegans are often thought of as simple...

  10. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...development of phosphoserine incorporation Extensive genomic sequencing of prokaryotic lineages revealed a curious...effects on cell growth. The C321.ΔA strain was genomically recoded to eliminate all 321 amber stop codons...Pirman, Natasha L., et al. "A flexible codon in genomically recoded Escherichia coli permits programmable...PMCID: PMC4566969. 7. Lajoie, Marc J., et al. "Genomically recoded organisms expand biological functions...

  12. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...samples in our queue such as viral samples for Viral Genome Sequencing (VGS). QC is performed throughout the...yield sufficient data to capture the entire viral genome. The full details of that workflow and its analysis...Platt RJ, Brigham MD, Sanjana NE, Zhang F (2017) Genome-scale CRISPR-Cas9 knockout and transcriptional ...

  13. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...sensors, our approach uses portions of the pathogen’s genome as unique barcodes to identify samples containing... Of the 48 sensors, targeting 24 regions of the genome, we have deposited the best performing sensors ...encoding corresponding portions of the Zika virus genome with Addgene to facilitate rapid sharing of these... Sample 6 Cofounder Michael Koeris Learn How Minigenomes Are Being Used to Study Dangerous Viruses Resources...

  14. Editor's Choice, September 2016

    Type
    Blog Post
    Published
    Oct. 7, 2016, 4 p.m.
    ...useful to your colleagues. Technique: Probe Phage Genomes for Host Binding Proteins & Running for Rare Disease...this month. The first, "Technique: Probe Phage Genomes for Host Binding Proteins," was contributed by ...

  15. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...RABV, these sequences are not copied into viral genome, so viral progeny will not necessarily express ...can be achieved by modifying the wild-type RABV genome, which encodes a single envelope protein called...incorporated into the viral envelope (but not the viral genome). Nascent G protein-coated RABV is then released...

  16. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...desired genetic modification integrates into the genome (Figure 3). Here, the chromoprotein is expressed...tsPurple) color. Though the toxin is integrated in the genome, it is not expressed as it is under the control...for the Scarless Modification of Proteobacterial Genomes. Applied and Environmental Microbiology, 85(16)...

  18. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    Published
    July 20, 2017, 2:30 p.m.
    ...these patients suffer any less acutely.  The Rare Genomics Institute works to help those who suffer from ...disease cures. Through their BeHEARD Award, the Rare Genomics Institute partners with companies to provide technological...

  20. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...considered an authority in this area. What is genome engineering? How does CRISPR work? This section...that, like computers and the internet before it, genome engineering technology is moving along much faster...examples of how understanding genetics is so timely.” Genome engineering, and many other technologies and fields...
Showing: 221 - 240 of 411 results