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We narrowed to 488 results for: mme

Showing: 221 - 240 of 488 results

  1. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ... mycoplasma contamination. There are several commercially available mycoplasma detection kits that use... example, while freeze thaw is generally not recommended, Holic et al. found that lentivirus produced ...

  2. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...something to brighten our day, whether it’s memories of summer or just a bright new plasmid. One area with lots...about your favorite new biosensor plasmids in the comments below!   Additional resources on the Addgene ...

  3. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...antibodies and refer to the manufacturer’s recommendations.  As you optimize your protocol, always remember...To learn more about antibody validation, this commentary by Uhlen, et al. 2016 is a good place to start...

  5. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...countries around the world and to do so, the WHO recommends one strategy: testing, tracking, and social distancing...recombinase polymerase amplification (RPA) using a commercially available amplification kit. In this case, regions...

  6. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...improve his eBook, adds: "If you have any questions, comments or suggestions, please do not hesitate to email.... These should be plainly stated. I would not recommend putting these into slide format. You want people...

  7. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...into the field. Current versions are already commercially available although they are reported to have...your favorite "Historical Lab Technique" in the comments section below! Many thanks to our guest blogger...

  8. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    Published
    Oct. 2, 2025, 1:15 p.m.
    ...Marketing Manager at Promega. His work focuses on commercializing their cell and protein analysis products. You... M. P., Butler, B. L., Wood, M. G., Otto, P., Zimmerman, K., Vidugiris, G., Machleidt, T., Robers, M. ...

  9. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ... before you start an experiment with CRISPR The hammer, the jigsaw, and the wrench are all great tools...ignored. Created with BioRender.com. The hammer: Gene knockout by NHEJ Gene knockout with CRISPR...

  10. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...just natural differences between individuals. Recommended controls for antibody-based experiments Primary...above, viability and compensation controls are recommended for flow cytometry. A viability control allows...

  11. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...predict the perfect titer, but one of the main recommendations when using AAVs is to avoid freezing and thawing... ScAAV avoid this rate limiting step and can immediately undergo replication and transcription. However...

  12. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...distinguish a signal from noise; therefore, it is recommended to use brighter fluorophores for markers that...marker to be highly abundant, it is fine to use a dimmer fluorophore. However, these aren't strict rules...

  13. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ... Additional libraries are also available from commercial vendors. These libraries usually contain 4-12...community. It shows how science can be accelerated immensely when we share knowledge and reagents. I have not...

  14. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...inactivated and less photons are emitted leading to dimmer fluorescence. In fact, many of the early reversible... a favorite use for PA-FPs? Let us know in the comments section below! Currently, there are over 20 different...

  15. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...residues. While these Cy/NTA conjugates are not commercially available, they are easily synthesized with ...Griffin, Adams, & Tsien, 1998), these dyes are now commercially available as Lumio Green and Lumio Red from ...

  16. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ... are accurate and reproducible. I also highly recommend spending the money on a high quality stereotaxic...optimal time period between viral injection and commencement of behavioral experimentation.  Whether you ...

  17. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    Published
    June 2, 2017, 2:30 p.m.
    ...information that's out there, Addgenie Leila Haery has summed up some of the most important characteristics of...

  18. A Case Study of Addgene Bioinformatics

    Type
    Blog Post
    Published
    May 25, 2023, 1:15 p.m.
    ...Last summer, we posted about our bioinformatics quest, which led us to Seqera Labs, a company that provides...

  20. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    Published
    Dec. 28, 2021, 2:15 p.m.
    ...the seventh day of CRISPR, Addgene gave to me: programmed genome editing by a miniature CRISPR-Cas12f nuclease...
Showing: 221 - 240 of 488 results