We narrowed to 488 results for: mme

... mycoplasma contamination. There are several commercially available mycoplasma detection kits that use... example, while freeze thaw is generally not recommended, Holic et al. found that lentivirus produced ...

...something to brighten our day, whether it’s memories of summer or just a bright new plasmid. One area with lots...about your favorite new biosensor plasmids in the comments below!   Additional resources on the Addgene ...

...antibodies and refer to the manufacturer’s recommendations.  As you optimize your protocol, always remember...To learn more about antibody validation, this commentary by Uhlen, et al. 2016 is a good place to start...

....org/10.1038/mt.2010.205 Trapani I, Colella P, Sommella A, Iodice C, Cesi G, Simone S, Marrocco E, Rossi... Simone S, Colella P, Iodice C, Polishchuk EV, Sommella A, Colecchi L, Rossi S, Simonelli F, Giunti M,...

...countries around the world and to do so, the WHO recommends one strategy: testing, tracking, and social distancing...recombinase polymerase amplification (RPA) using a commercially available amplification kit. In this case, regions...

...improve his eBook, adds: "If you have any questions, comments or suggestions, please do not hesitate to email.... These should be plainly stated. I would not recommend putting these into slide format. You want people...

...into the field. Current versions are already commercially available although they are reported to have...your favorite "Historical Lab Technique" in the comments section below! Many thanks to our guest blogger...

...just natural differences between individuals. Recommended controls for antibody-based experiments Primary...above, viability and compensation controls are recommended for flow cytometry. A viability control allows...

...Marketing Manager at Promega. His work focuses on commercializing their cell and protein analysis products. You... M. P., Butler, B. L., Wood, M. G., Otto, P., Zimmerman, K., Vidugiris, G., Machleidt, T., Robers, M. ...

... before you start an experiment with CRISPR The hammer, the jigsaw, and the wrench are all great tools...ignored. Created with BioRender.com. The hammer: Gene knockout by NHEJ Gene knockout with CRISPR...

...predict the perfect titer, but one of the main recommendations when using AAVs is to avoid freezing and thawing... ScAAV avoid this rate limiting step and can immediately undergo replication and transcription. However...

... Additional libraries are also available from commercial vendors. These libraries usually contain 4-12...community. It shows how science can be accelerated immensely when we share knowledge and reagents. I have not...

...inactivated and less photons are emitted leading to dimmer fluorescence. In fact, many of the early reversible... a favorite use for PA-FPs? Let us know in the comments section below! Currently, there are over 20 different...

...distinguish a signal from noise; therefore, it is recommended to use brighter fluorophores for markers that...marker to be highly abundant, it is fine to use a dimmer fluorophore. However, these aren't strict rules...

...residues. While these Cy/NTA conjugates are not commercially available, they are easily synthesized with ...Griffin, Adams, & Tsien, 1998), these dyes are now commercially available as Lumio Green and Lumio Red from ...

... are accurate and reproducible. I also highly recommend spending the money on a high quality stereotaxic...optimal time period between viral injection and commencement of behavioral experimentation.  Whether you ...

...information that's out there, Addgenie Leila Haery has summed up some of the most important characteristics of...

...Last summer, we posted about our bioinformatics quest, which led us to Seqera Labs, a company that provides...

...seem to find an antibody that works, we highly recommend “Chapter 3: Finding the Right Antibody for Your...

...the seventh day of CRISPR, Addgene gave to me: programmed genome editing by a miniature CRISPR-Cas12f nuclease...