We narrowed to 689 results for: mos

...year were synthetic. They also found out that the most common expression system within the Addgene repository...of unique gene sequences is Proteobacteria - the most frequent transfer of genes is from Proteobacteria...biology page Search for a plasmid Find Addgene's most popular plasmids ...

...non-academic jobs as “alternative” anymore.   Since most scientists start out in academia, it is especially...waiting Work is concentrated to business hours (mostly), but can often achieve more structured time management...and what parts of being a scientist you enjoy the most. Having a better idea of where you want to go will...

... development as your mentor. How do you make the most of this new relationship? Consider adding formality... a mentoring relationship really productive, the most important step you can take is to set goals to work...teaching to help a Mentee reach goals. One of the most important contributions made by a Mentor is to help...

... plasmids. These libraries are some of Addgene’s most exciting and versatile offerings! We recently re-amplified...protocol, but the overall workflow is similar for most libraries. Here are the key points you’ll need to...Figure 3A). Addgene finds that this representation is most easily understood visually. As an example, the curves...

...cell differentiation fate to tumor suppression (and most everything in between), understanding how, when,...selections In its current format, the MORF library is most immediately useful for TF library screens. Previously... More about Lentivirus Figure out How to Get the Most from Your Lentiviral Transduction Additional Resources...

...of heat-inducible lentiviral CAR constructs, the most basic of which is depicted here: The heat-inducible...and, as you might suspect, it has been one of our most commonly requested antibodies. But sometimes, one...temperatures, and other chaotropic conditions better than most FPs. The group also tested hfYFP in expansion microscopy...

...fluorescent proteins (FPs) have become one of the most indispensable tools in modern biological research...probe (be it an organic dye or FP) is one of the most important steps in the design of an imaging experiment...soon as possible after submission. Using FPbase The most obvious use case for FPbase is finding information...

...presented chronologically. With a resume however, most often people choose a reversed chronological style...often I see resumes from good candidates that I almost missed because I had to “dig” hard for the information... in the News Talk to your career services office Most universities actually have career services offices...

..., including dendritic and axonal extensions and, most importantly, any connections the neurons make to...capable of spreading across synaptic connections. Most famous is the Rabies Virus (RABV) which moves retrogradely...  Commonly used serotypes include AAV1 – 9, with most of these serotypes capable of entering neurons (...adeno-associated viral vector serotypes 1, 2, 5, 8 and 9 in marmoset, mouse and macaque cerebral cortex, Neurosci Res...

...For most people, the most familiar source of bioluminescence is the firefly. Along with charming nighttime...Many luciferase assays require cell lysis as the most efficient means to disrupt the cell membrane and...

...classroom cannot offer. Furthermore, companies across most industries tend to hire their former interns to ...assess your eligibility, interest, and career fit. Most internships share a common set of criteria such ...background, and lighting for a smooth performance. Most importantly, make certain that everyone you live...

... RNA samples. RNA is inherently less stable than most macromolecules used in scientific research such ...with post translational modifications. One of the most common of these is 2’-O-methylation, where a methyl...major drawback of CRISPR-Cas9 technology is that most expression systems are constantly “on”. There is...

...injected 1e+13 vg per mouse to get phenotypes (that’s almost 100ul/animal for the PHP.eBs that we have in house...expression is when injecting CAP-B10 or CAP-B22 in marmosets, from an individual to another individual perspective...CAP-B10 and CAP-B22 were tested individually in 4 marmosets each. Brain potency for each individual is reported...

...each other until you are ready to separate them. Most proteins are not likely to remain attached to the...want to break them. Crosslinking is necessary for most ChIP experiences, but the increased binding can ...and complex, you may be thinking, well, these are mostly fairly standard molecular and cell biology techniques...

...Addgene’s content team, we — Jo, Brook, and Angela — most often think about accessibility in terms of the ...working together to share information. Sometimes it is most helpful to use a lot of jargon and have a technical...takeaway.  In this image, we thought it would be most helpful to highlight the technical details of collecting...

...? There have been several systems to do this but most are labor-intensive, require multiple fluorescent...They continued to use GEmCherry2 to identify the most efficient sgRNAs to target a specific locus to create...efficiency. So if you are looking to identify the most effective gRNA and enrich cells for your CRISPR ...

...genetic modification by homologous recombination. In most recombineering strategies, only 3-4 phage proteins...a whopping 144 kb from a bacterial artificial chromosome in just one reaction. Using other methods, such... which are recommended for plasmids up to 25 kb, most cloners should be fine, especially using plasmids...

... write them on a board to see which ones get the most hits. Commit to a meeting schedule and meet regularly...ready for the discussion with resources. One of the most common questions I get is, “What are we going to...– compete for fastest to set up a coffee date or most cards collected. The next blog in this series will...

...systems, making it well suited for AAV packaging. Most importantly, this CasMINI system was shown to be...compact genome editors and a gRNA down-sized by almost 40%. The activity of Cas12f1 with these modified...of the system to optimize its outcomes. In their most recent work, the Liu Lab boosts the efficiency of...

... expression, you are almost certainly working with an endogenous locus. The most straightforward way to...function at the post-translational level, unlike most of the other mechanisms described. Fig. ...