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We narrowed to 662 results for: mpo

Showing: 221 - 240 of 662 results

  1. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...well as the introduction of the cancer-fighting component, CAR. Clinical trials are underway to explore ...effectiveness, and delivery. Patient safety is important for allogenic therapies to prevent a graft vs....vs. host response. Off-target effects are also important safety considerations for all CRISPR therapies...

  2. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...Calcium is arguably one of the most important metal ions in biology (sodium and potassium are up there...blood clotting, and as we all know, a structural component of bones and teeth. It is an enzyme co-factor,..., and an allosteric modulator. Because of its importance, scientists have developed calcium sensors to...

  3. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ..., and North America. Diagnostics will play an important role in helping to monitor and slow this spread...American-lineages). Strain discrimination is especially important in the current outbreak because of the neurological...of Zika Virus Using Programmable Biomolecular Components." Cell (2016). PubMed PMID: 27160350. Find Plasmids...

  4. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...that infect bacteria), many of which are doing important work in our microbiomes. Given that viruses are...undergo DNA rearrangements. Depending on the composition of the viral vector and the repeat sequences ...ensuring viral production is to perform PCR against components of the viral vector using the harvested viral...

  5. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...was the outdoor market where one can shop for components of traditional Chinese medicine. There were hundreds...field. Nobel Laureates and the ever expanding importance of RNA The second keynote lecture was presented...in his lab. RNA is a structural and functional component of the ribosome complex and Dr. Steitz quipped...

  6. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    Published
    Jan. 21, 2015, 1:30 p.m.
    ...-aggressives, who are usually antagonistic and impolite, Complainers, who spend most of their time moaning...solutions that will meet both of your needs. 6) Put important agreements in writing One of the most frequent...from your supervisor. Whenever possible, discuss important issues in person or over the telephone instead...

  7. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    Published
    Oct. 11, 2018, 12:30 p.m.
    ...setting up assembly reactions in which a single component was purposefully omitted. Since any assembly is... no blue colonies were obtained if any single component was omitted. Screening for successful Golden ...The future of assembly DNA assembly methods are important tools for many areas of science, and researchers...

  8. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ... purposes. We’re here to help with that! Four important factors to consider for any experiment are: ...Efficiency For cloning in particular, another important consideration is: The number of inserts ......, like Gibson or SLIC, since one can pool the components and define their final order through sequence...

  9. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    Published
    July 13, 2017, 2:30 p.m.
    ...combs. To make genetic crosses properly, it is important to isolate females from males while they are still... after hatching into their adult phase. It is important to clear out the parent crosses from the container...feature when setting up genetic crosses. More importantly, this knowledge will stop you from embarking ...

  10. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...publishing is important to you, make sure that your project will not involve proprietary compounds or information...communicate your science to others and advocate for the importance of your work. In my experience in industry, you...

  11. Hot Plasmids: Summer 2025

    Type
    Blog Post
    Published
    Aug. 28, 2025, 1:15 p.m.
    ...variety of constructs for visualizing cellular components (nucleus, endosomes, lysosome, golgi, ER, inhibitory...inhibitory synapses, and cytoskeleton components) and in a FRET-based biosensor for cAMP dynamics. The ... synapses; however, its modular design allows components to be swapped out, potentially broadening its...

  12. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    Published
    Dec. 12, 2013, 3:35 p.m.
    ...carefully engineered to support reliable functional composition. In other words, these parts can be used over...YouTube primer on BIL gates.) Addgene: Why is it so important to you that these parts be made freely available...language is put into practice? Endy: I think it's important to understand that these platforms, or pieces ...

  13. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...presentation because, in my opinion, it was more important to communicate the applications of CRISPR over...use it? This last section was arguably the most important, and it was a little worrisome for me since bioethics...biology, are moving so quickly that it’s basically impossible for textbooks to keep up. Even the most ambitious...

  14. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...analyzable transgenic in hand, so it is pretty important to get this process right the first time. I’ll...to transform when they begin flowering. It is important to perform the transformation not long after flowering...s time to select for transformants. It’s very important to select multiple T1 plants from each transformation...

  15. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...gut, and other organs to the CNS, are also an important target for gene delivery, especially for the study...3293995. Additional Resources on the Addgene Blog Important considerations when using AAVs Learn more about...research. Visit Our AAV Guide Page for info on AAV components, common uses of AAV, and much more! Find ready-to-use...

  16. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...by ddPCR begins with diluting the virus. It is important to note that the dynamic range of the ddPCR is...PCR plate. Maintaining a high droplet count is important for calculating the concentration of your sample...separation (Witte et al., 2016). This is particularly important if your amplicon is longer than 150 base pairs...

  17. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...your samples, the relationships between cellular components, and the accessibility of your target epitope...work by dehydrating and precipitating cellular components. This method of fixation is good for epitopes...away your target (for example, some cytoskeletal components) and you want to fix and permeabilize in one ...

  18. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...is being involved in the scientific community important to you and your lab? Fujii: I first knew Addgene...interact with each other, which is critically important, especially for young researchers. It was a really...technologies. Majority of them are eager to just "import" technologies developed abroad, so it's a little...

  19. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    .... More importantly, the expression of this construct is Cre-dependent, enabling spatiotemporal control...are needed to deliver all the protein and RNA components, and several sgRNAs must be screened to identify...

  20. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...valuable lessons along the way. We think it’s so important that we have written plenty of articles on building...bench mentor-mentee relationship is extremely important – without a good set up it could be very difficult...help you get more out of the in-person training component. Documenting during training It’s hard to recall...
Showing: 221 - 240 of 662 results