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We narrowed to 745 results for: nes

Showing: 221 - 240 of 745 results

  1. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    Published
    April 1, 2014, 5:33 p.m.
    ...dictator or laissez-faire; theory or practice; business-like or artistic; tech, anti-tech or pragmatic...employers love PhD students to have: working to deadlines, writing up clear and attractive reports, being...students and postdocs go on to work in patent law, business development, teaching, etc. Church also encourages...science and engineering (STEM) expertise in law, business, journalism, entertainment, politics, K-12, etc... passion, hard work, collaboration, and staying honest with personal goals. Stay tuned for the follow ...

  2. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    Published
    Aug. 22, 2019, 12:43 p.m.
    ...naturally occurring genes for cloning purposes (antibiotic resistance genes, toxin genes, and the sacB gene...gene of interest, but others will uptake plasmid backbones that re-ligated back on themselves. Therefore,...Plasmid cloning: Creativity with naturally occurring genes  At this point in the blog post, you may have realized...

  3. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...explosion SUperNova," SunTag can help you turn up the brightness in your fluorescent imaging experiments. Fluorescent...managed to track run lengths of the motor protein kinesin across microtubules. Tanenbaum et al. then tested...to traditional fluorescent proteins Improved brightness and signal-to-noise ratio Less chance of phototoxicity...used to improve CRISPR-based activation of target genes, but we’ll save that application for another day...on Addgene.org Find Fluorescent Protein Empy Backbones Visit Our Fluorescent Protein Guide Page Find ...

  4. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...both introduce genes into an organism’s genome (see figure) and to disrupt endogenous genes at the site ...by transposition assays of antibiotic-resistance genes conducted in HeLa cells). In their Nature Genetics... the rapid generation of stable transgenic cell lines which can robustly express your gene-of-interest...transposons rapidly generate stable transgenic cell lines. Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821...

  5. Advice for Choosing a Research Project

    Type
    Blog Post
    Published
    April 17, 2014, 2:45 p.m.
    ...research project must have innovative yet attainable milestones that contribute to intellectual thought and discovery... and skills do you need to use to reach these milestones? Can you get assistance from people who have ...Think about what your personal goals are and set milestones along the way so that you don't get stuck doing...been developed. Can you think of a project that combines an exciting new method or approach with another... Passion fuels the drive to continue persuing milestones and working hard. Being patient, being selective...

  6. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ...Now that we know all about antibiotic resistance genes, let’s consider another basic element of any plasmid...focused only on those found in bacteria. Some common ones you might see include ColE1, pMB1 (which comes in...20-40 CloDF13 (CDF) D Relaxed This table defines common cloning vectors, their copy number, ori,...copies of plasmids if they contain large inserts or genes that create a toxic product. The E. coli strain...for single- and multiple-unnatural amino acid mutagenesis in Escherichia coli. Biochemistry. 2013 Mar 12...

  7. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ...validated simultaneously in the brain and the intestines of mice. The result? A very cool tool to advance...polymers are heated up and pulled to a desired thickness. The fibers are drawn with microelectronics included...engineering road map of sorts, giving an idea of the stiffness the fibers would need to be, as well as other ...fiber implants. Due to the soft nature of the intestines and the requirement for digestive motility to...excited a class of enteroendocrine cells within the intestines known as neuropods. They observed increased vagus...

  8. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    .... Yet, we cannot prosper in a world where our medicines, technologies, defense, and governing policies...more scientists in our leadership, and raising awareness regarding the pitfalls of accepting alternative...chromosomes. Montreal, QC, Canada 45.5017°N 73.5673°W – Vanessa Sung Hundreds of scientists and supporters in ...well as allies outside the scientific community. Vanessa Sung is currently a PhD student studying breast...restorative justice.  Follow her on twitter @sung_vanessa. Albany, NY, USA 42.6526°N 73.7562°W – Nicholas...denying science, from celebrities falsely claiming vaccines cause autism to politicians saying that climate...

  9. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...mice Transgenic mouse lines have also been engineered to express various opsin genes [7-9]. The advantage...other tool in a scientist’s vast toolbox, its effectiveness depends on how and why it’s used. There are ...behavioral output is sufficient to assess the effectiveness of your optogenetic manipulations (though a ...has genetically manipulated a number of the opsin genes to alter the light spectrum for activation and the...advantage of using these lines is, if one is crossed with an appropriate mouse line expressing a particular ...

  10. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

    Type
    Blog Post
    Published
    Sept. 3, 2019, 1:15 p.m.
    ...Cecchetelli Auxin Inducible Degradation (AID) uses plant hormones called auxins to conditionally and reversibly ...AID to yeast, as well as mammalian and human cell lines (Nishimura et al., 2009, Natsume et al., 2016).  ...protein of interest to create conditional human cell lines. Lastly, the Kanemaki lab demonstrated that auxinole...targeted by the Cas9 nuclease. The Streptococcus pyogenes Cas9 (SpCas9) requires an NGG PAM to recognize... role in the transcriptional regulation of many genes. A number of groups have designed means of studying...

  11. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    Published
    Nov. 27, 2018, 2 p.m.
    ...Julio Ernesto Valdivia Silva, Universidad de Ingeniería y Tecnología (UTEC), Peru Julio Ernesto Valdivia...her current research, Sheen aims to identify new genes related to PZA-resistance by using different approaches...experience in the field of bacterial and fungal pathogenesis. Her research focuses on a collection of pathogenic...growth will then be measured to link the deleted genes to specific functions in biofilm formation. The...

  12. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ...provide the Brunello (targeting human genes) and Brie (targeting mouse genes) pooled libraries from the labs...popular genome editing tool is its developers’ willingness to make their CRISPR technologies available to...pooled gRNA libraries that can be used to screen for genes involved in a wide variety of cellular processes...80,000 gRNAs to target around 20,000 individual genes. We provide enough lentivirus for you to both perform...

  13. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ...when choosing an individual fluorescent protein (brightness, photostability, and so on; see the previous ...use (particularly not the most recently published ones), they can be a good starting point. In many cases... used filter sets for multicolor imaging include ones designed for CFP, YFP, and RFP or the Sedat Quad...supplement your cells with biliverdin for maximal brightness. In mammalian cells, one of the improved folding...proteins with this filter set and have reported brightness measurements. Here, EGFP outperforms the improved...

  14. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...stable cell lines and are not used for general plasmid selection. Instead, reporter genes are often employed...within the cell for effective replication. Cell lines expressing the Epstein–Barr virus (EBV) nuclear ...48 h post transfection when creating stable cell lines. Looking for Mammalian Expression Plasmids? Check...Read More: Plasmids 101: Antibiotic Resistance Genes Plasmids 101: Origin of Replication All Plasmids...

  15. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type
    Blog Post
    Published
    May 19, 2020, 1:15 p.m.
    ...global pandemic in which scientists race to find vaccines, therapeutics, and other treatments to stem the...plasmids containing all SARS-CoV-2 protein coding genes in E. coli and S. cerevisiae expression vectors ...yeast expression constructs contain protein coding genes, tags, truncations, and codon optimizations similar...plasmids. Each combination is present in two sets of backbones which drive expression using either TEF1 or GAL1...of plasmids containing SARS-CoV-2 protein coding genes in E. coli and S. cerevisiae expression vectors ...

  16. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...target gene, it therefore determines the timing of gene expression and largely defines the amount of recombinant... cAMP and represses pBad, decreasing promoter leakiness.   Other examples of chemically induced promoters... be compatible with your experimental system. Leakiness If you’re expressing a gene that may be toxic,...inducible promoters are also known for their low leakiness. Inducibility level How much do you want to induce...

  17. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    Published
    June 25, 2019, 12:54 p.m.
    ... comic, when a bacterium uptakes a plasmid, the genes on the plasmid can give them different phenotypes...example are bacteriophages that attach to bacterial membranes and inject their genetic material into the cell... DNA into eukaryotic cells, like mammalian cell lines. This can be done with lentiviral and Adeno Associated...and AAV can be used to create both transient cell lines, where a gene of interest is expressed but not integrated... integrated into the genome and stable cell lines, where foreign DNA is incorporated into the cell’s genome...

  18. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ...single-use” transgenic lines. Instead, researchers can generate and maintain transgenic lines that can be used...system that makes use of pre-existing transgenic lines, termed BLITZ (Biotin Labeling In Transgenic Zebrafish...dCas9 and microbial SSAPs (dCas9-SSAP) which insert genes in a cleavage free manner. The dCas9-SSAP system... 12-plex multiplex imaging system. Mixing the subclones within a single well reduced well-to-well variability...

  19. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ... nucleic acid sequence of the antibody-encoding genes over time as the hybridoma cells divide. These changes...hybridoma cells before culturing or they can clone the genes from the hybridoma into a plasmid to create a recombinant...Figure 2: Changes to the heavy chain or light chain genes within a hybridoma impacts the specificity, avidity...Wilkinson IC, Tandon AK, Anderson S, Bladen CL, Jones B, Aldred SF, Bestagno M, Burrone O, Maynard J, ...1999. 1, Generation of Hybridomas: Permanent Cell Lines Secreting Monoclonal Antibodies. https://www.ncbi.nlm.nih.gov...

  20. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...transcription of other relevant viral genes (including the ones listed previously). E3/E1B suppresses ...applications and aid in ease of use. Our lentiviral guide outlines these components.     Fig. 1 - Viral vector... AAV also requires some back up from adenovirus genes – E4, E2a, and VA – which are supplied on a helper...viral replication. More information on these viral genes is available in the adenovirus section below!   ...
Showing: 221 - 240 of 745 results