We narrowed to 507 results for: otos
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TypeBlog PostPublishedJuly 20, 2017, 2:30 p.m....gene results in the degeneration of spinal cord motor neurons. This disease is estimated to affect anywhere...molecules for use in the clinic and thereby restore motor neuron function in spinal muscular atrophy patients...
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Scientific Sharing in the Time of COVID-19: Databases and Resources
TypeBlog PostPublishedMay 12, 2020, 1:15 p.m....reagents, funding and volunteer opportunities, protocols, publications, and more to accelerate the pace...scientific community work together to share open protocols, reagents, and other initiatives for #COVID19 ... -
IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging
TypeBlog PostPublishedJune 8, 2023, 1:15 p.m....vision is an open, global repository for reagents, protocols, panels, publications, software, and datasets....high-content imaging of diverse tissues. Nature Protocols, 17(2), Article 2. https://doi.org/10.1038/s41596... -
Build Your CRISPR Vocabulary
TypeBlog PostPublishedJuly 2, 2024, 1:15 p.m....integration into the array, these sequences are called protospacers. Repeats: Bacterial DNA that appears between...sequence matches the guide sequence. PAM: The “protospacer-adjacent motif” present in the non-target strand... -
The Blue Flame Award: Celebrating Addgene's Most Requested Depositors
TypeBlog PostPublishedNov. 14, 2016, 3:30 p.m....at Hiroshima University who works with Takashi Yamamoto. “Addgene has made it possible to eliminate geographical...we’d love to see you show it off! To share your photo, tag @Addgene and #happydepositor on Twitter. All... -
Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process
TypeBlog PostPublishedFeb. 1, 2018, 3:07 p.m....Pages Basic Molecular Biology Protocols Diagnostic Restriction Digest Protocol ... -
Sensing Neuronal Dopamine
TypeBlog PostPublishedJuly 30, 2019, 12:59 p.m....in reinforcement learning, decision making, and motor control. What do we understand about Dopamine?...Learn about Addgene's viral service Find viral protocols and videos Find biosensor AAVs for your research... -
The Stingy Scientist: How the Baby Gel Box Was Born
TypeBlog PostPublishedApril 15, 2014, 3:11 p.m.... the most accessed blogs on BitesizeBio is the protocol for making your own Taq polymerase by Outreach...Addgene founder, Melina Fan, recently shared our protocol for making competent cells to save time and money... -
Addgene Begins Distribution of Recombinant Antibodies
TypeBlog PostPublishedMarch 28, 2022, 4:30 p.m....recombinant antibodies, educational materials, protocols, and shared data, on our values of transparency... antibody collection. Addgene is sharing our protocols for recombinant antibody production as well as... -
RUBY-Red Siliques
TypeBlog PostPublishedMarch 1, 2022, 2:15 p.m.... Figure 1: An adult plant expressing RUBY. Photos by Andrew Willoughby. Using RUBY as a selection...green tissues is slightly darker or slightly brown. Photos by Andrew Willoughby. The ease of observing... -
Using Addgene's New Viral Service for Your Research
TypeBlog PostPublishedNov. 17, 2016, 3:30 p.m.... viral vector pages as well as expanded virus protocols, information on how we produce and test virus,...Overview of Viral Production at Addgene Find Virus Protocols Browse All Viral Vectors ... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog PostPublishedJune 2, 2020, 1:15 p.m.... intercalating fluorescent dyes which can cause photo-induced DNA breakage and structural deformation,...reversible and do not cause structural deformation or photo-induced damage. These fusions can also function ... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog PostPublishedApril 23, 2020, 1:15 p.m.... blog post on AAV titers Browse viral vectors protocols and tips Resources on Addgene.org Find AAVs ...Learn more about Addgene's viral service Find protocols for working with virus ... -
New and Upcoming Viral Vectors - December 2019
TypeBlog PostPublishedDec. 19, 2019, 2:40 p.m....localize opsins to the soma to avoid off target photostimulation of axons and dendrites on neighboring neurons...Learn about Addgene's viral service Find viral protocols and videos Find AAVs for your research ... -
Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation
TypeBlog PostPublishedDec. 13, 2018, 1:14 p.m....goal is to provide scientists with new tools and protocols that they can easily incorporate into their own...service Learn about viral production Find viral protocols here ... -
Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC
TypeBlog PostPublishedSept. 21, 2023, 1:15 p.m....endogenous IgGs in your tissue sample. Most common IHC protocols use two antibodies - one against your target of...… Another strategy for species-on-species IHC protocols is to block endogenous IgGs by pre-treating the... -
Antibodies 101: Epitope Tags
TypeBlog PostPublishedMay 11, 2023, 1:15 p.m.... acid peptide (EQKLISEEDL) derived from the protooncogene of the same name. The tag has a net negative...General Use Antibodies Antibody guide Antibody protocols Additional resources on the Addgene blog Antibodies... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog PostPublishedNov. 12, 2019, 2 p.m....CRISPR nuclease activity is desired and time or protocol steps can be saved by flexibly deploying IgnaviCas9...without an increase in the number of steps in the protocol. IgnaviCas9’s incredible thermostability makes... -
Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]
TypeBlog PostPublishedApril 7, 2015, 4:21 p.m....Database, bas006 (2012). PubMed. For a stepwise protocol on designing and using RNA scaffolds: Delebecque...scaffolds to assemble proteins in vivo. Nature protocols. 7(10) 1797-1807 (2012). PubMed. ... -
How Dry Ice Affects Viral Vector Sample pH & How to Avoid It
TypeBlog PostPublishedJune 20, 2017, 2:30 p.m....Byrne BJ, Zolotukhin S. A simplified purification protocol for recombinant adeno-associated virus vectors...Addgene.org Check out the Viral Service Find Virus Protocols Browse Our Viral Vectors ...