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  3. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    Published
    Dec. 19, 2019, 2:40 p.m.
    ...localize opsins to the soma to avoid off target photostimulation of axons and dendrites on neighboring neurons...Learn about Addgene's viral service Find viral protocols and videos Find AAVs for your research ...

  5. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    Published
    Nov. 17, 2016, 3:30 p.m.
    ... viral vector pages as well as expanded virus protocols, information on how we produce and test virus,...Overview of Viral Production at Addgene Find Virus Protocols Browse All Viral Vectors ...

  7. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ...recombinant antibodies, educational materials, protocols, and shared data, on our values of transparency... antibody collection.  Addgene is sharing our protocols for recombinant antibody production as well as...

  8. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ... Figure 1: An adult plant expressing RUBY. Photos by Andrew Willoughby   Using RUBY as a selection...green tissues is slightly darker or slightly brown. Photos by Andrew Willoughby The ease of observing ...

  9. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    Published
    Sept. 21, 2023, 1:15 p.m.
    ...endogenous IgGs in your tissue sample. Most common IHC protocols use two antibodies - one against your target of...… Another strategy for species-on-species IHC protocols is to block endogenous IgGs by pre-treating the...

  10. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ... acid peptide (EQKLISEEDL) derived from the protooncogene of the same name. The tag has a net negative...General Use Antibodies Antibody guide Antibody protocols Additional resources on the Addgene blog Antibodies...

  11. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ...Database, bas006 (2012). PubMed. For a stepwise protocol on designing and using RNA scaffolds: Delebecque...scaffolds to assemble proteins in vivo. Nature protocols. 7(10) 1797-1807 (2012). PubMed. ...

  13. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...Byrne BJ, Zolotukhin S. A simplified purification protocol for recombinant adeno-associated virus vectors...Addgene.org Check out the Viral Service Find Virus Protocols Browse Our Viral Vectors ...

  14. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ...specificity of influence measurements by reducing photostimulation of the axons and dendrites of non-targeted...Learn about Addgene's viral service Find viral protocols and videos Find AAVs for your research ...

  15. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ...RNA provided that target is upstream (5') of a protospacer adjacent motif (PAM) Cas9 endonuclease binding... RNA and a 3-base pair sequence known as the Protospacer Adjacent Motif or PAM. In order for dsDNA to ...

  17. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    Published
    Sept. 15, 2020, 1:15 p.m.
    ...you’re planning to go this route, check out our protocol video on using restriction digests to analyze ...Plasmids 101 series Find more molecular biology protocols and tips Resources on Addgene.org Learn more...

  18. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ...educational resources — including blog posts, protocols, guides, and collections — is a lot of material...plasmids and viral preps for catalog, blog posts and protocols for educational resources, and labs and institutions...

  20. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...Usually Destroyed Yes, Cpf1 cleaves 5' of the protospacer Multiplexing CRISPR-Cas9 options before Cpf1...Delivery Method Advantages Disadvantages References Yamamoto Lab Golden Gate Assembly transfection One vector...
Showing: 221 - 240 of 495 results