We narrowed to 263 results for: rigi

1. Transferable Skills Guide: Career Planning Resources

   Type

   Blog Post

   Published

   Nov. 14, 2017, 2 p.m.

   ...you don’t get the specific position he or she originally called about, you can keep in touch and reach...

2. Twenty Years of Sharing: Expanding Accessibility

   Type

   Blog Post

   Published

   Nov. 21, 2024, 10:47 p.m.

   ...Bacterial Expression Systems AAV Plasmids Blogs Origin of Replication What is a Plasmid? How to Design...

3. Antibodies 101: The Four ELISAs and When to Use Them

   Type

   Blog Post

   Published

   July 25, 2024, 1:15 p.m.

   ...target antigen in a sample. While Engvall et al. originally developed the ELISA assay to measure antibody...

4. Tips for Writing a Good Cover Letter

   Type

   Blog Post

   Published

   May 21, 2024, 1:30 p.m.

   ... you're ready to go! Good luck! This post was originally written by Maria Soriano-Carot in September 2016...

5. 5 Reasons to Use Reddit for Science Communication

   Type

   Blog Post

   Published

   Oct. 20, 2016, 2:30 p.m.

   ...Science subreddit is well moderated. One of our original worries about sharing science advice on Reddit...

6. Tips for CRISPR Gene Editing in Mice

   Type

   Blog Post

   Published

   June 28, 2016, 10:59 a.m.

   ... with RNAs encoding Cas9 into the oocyte. This original method was developed as the use of CRISPR/Cas9...

7. Plasmids 101: The Promoter Region – Let's Go!

   Type

   Blog Post

   Published

   April 3, 2014, 8:05 p.m.

   ... such as the antibiotic resistance gene and the origin of replication are necessary to replicate plasmids...

8. New Optogenetic Tools for Cytoskeleton and Membrane Control

   Type

   Blog Post

   Published

   Sept. 28, 2023, 1:15 p.m.

   ... 10, 30, 50, and 100 times the activity of the original, generated using directed evolution (Tei et al...

9. Tips for Getting a Faculty Position

   Type

   Blog Post

   Published

   July 20, 2023, 1:15 p.m.

   ... at [email protected]." You can find his original blog post below! --Ed.  Eight years ago, I decided...

10. Quick Guide to Near-Infrared Fluorescent Proteins

    Type

    Blog Post

    Published

    Oct. 5, 2021, 1:15 p.m.

    ...normalization to fluorescence of co-transfected EGFP. e Originally reported as a monomer16, IFP2.0 was later found...

11. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type

    Blog Post

    Published

    Dec. 2, 2019, 2 p.m.

    ...did not affect the function or efficiency of the original APEX (Lam et al. 2014). They named this mutant...

12. Harnessing Bacterial Toxins for Allelic Exchange

    Type

    Blog Post

    Published

    Aug. 15, 2019, 12:30 p.m.

    ...chloramphenicol resistance cassette, and the R6K origin of replication to allow replication only in hosts...

13. CRISPR 101: Validating Your Genome Edit

    Type

    Blog Post

    Published

    Nov. 3, 2022, 12:15 p.m.

    ...This post was originally written by Melina Fan and updated Nov 3, 2022 by Susanna Stroik. You’ve designed...

14. Five Popular Model Organisms

    Type

    Blog Post

    Published

    April 11, 2019, 12:39 p.m.

    ...actually two distinct populations of β-cells that originate in different regions of the pancreas and produce...

15. The Advances Behind The World's First Personalized CRISPR Treatment

    Type

    Blog Post

    Published

    Sept. 16, 2025, 1:15 p.m.

    ...needed! Minimizing off-target editing ABEs were originally developed from RNA deaminases, using directed...

16. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type

    Blog Post

    Published

    May 13, 2025, 1:15 p.m.

    ...maintained in dividing cells because they lack an origin of replication (Banasik and McCray, 2010). However...

17. Hot Plasmids: Summer 2024

    Type

    Blog Post

    Published

    Aug. 27, 2024, 1:15 p.m.

    ... each other, while the 5’ flaps containing the original DNA sequence anneal with each other. Excision ...

18. Antibodies 101: Beyond Surface Labeling

    Type

    Blog Post

    Published

    June 27, 2024, 1:15 p.m.

    ...blotting, a primary epitope-targeting antibody can originate from a range of species; for example, rabbit or...

19. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type

    Blog Post

    Published

    April 4, 2024, 1:15 p.m.

    ...expected size. The strong signals close to the graph’s origin mainly stem from cell debris and other small fragments...

20. Fluorescent Proteins 101: When GFP lets you down

    Type

    Blog Post

    Published

    Aug. 23, 2018, 12:05 p.m.

    ... introducing two mutations (F64L, S65T) in the original jellyfish GFP (AvGFP) to create enhanced GFP (...