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We narrowed to 512 results for: sam

Showing: 221 - 240 of 512 results

  1. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...Because we have a resource like Addgene to all get the same virus.” — Grad student visiting Addgene’s booth ...quality reagents, and we realized we could use the same principles to provide recombinant antibodies, another...

  2. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...episomal and does not divide and replicate in the same way the host DNA does. Because of this, fast dividing...McCarty, D. M., Paul E. Monahan, and Richard J. Samulski. "Self-complementary recombinant adeno-associated...

  3. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...overall inflation of USD has gone up 19.8% in the same time period. For ease of calculations*, let’s split...inflation at… roughly 34.9%, which means that the same plasmid would cost $155.25 in reagents and consumables...

  4. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...systems can lead to local dsRNA production. In the same vein, genotoxic stress, such as chemotherapy treatment...dsRNA finds its way outside of the mitochondria, the same inflammatory response can activate. These types ...

  5. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...communication. Researchers often post a preprint at the same time as, or before, submitting the manuscript to...plasmids that were used in a preprint provides all the same benefits as sharing those plasmids alongside a journal...

  8. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...contact Addgene. We may require that you send your samples to Addgene pre-transformed in this strain. ...with instructions on how to prepare and ship the samples to Addgene and contact your technology transfer...

  10. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...necessary and sufficient for retrograde transmission (Etessami et al., 2000, Wickersham et al., 2007). Thus, ...Mar 19;110(12):E1152-61 Pubmed PMID 23487772. 3. Etessami R, Conzelmann KK, Fadai-Ghotbi B, Natelson B, ...

  12. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...your poster. While a poster generally contains the same sections as a primary research article, it’s important...four column style, and in all cases the idea is the same; the reader will navigate the poster from column...

  13. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...& Walton et al). Additionally, SpG Cas9 with the same PAM preference of NG was isolated by the Kleinstiver...several bps away from a desired site does not have the same devastating impact on efficiency as 10-15 bps away...

  14. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ... viruses are more dangerous than others, and the same holds true for types of viruses generated in the...personal protection are key to lab safety and the same principles apply to viral handling. Gloves are always...

  16. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...between the sites. If a DNA sequence is floxed in the same orientation, the SSR will excise the DNA fragment...reporter with one loxP site and one lox511 site in the same orientation, and placing the additional loxP and...

  17. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ...answers uninteresting, the panel members all say the same thing and one panel member dominates the discussion... for an intimate event, put the panelists on the same level as the audience. In my experience the best...

  18. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ... synthetic biology lab Keira and I worked in the same lab for four years. It was a cutting-edge synthetic... on the detector plants, we expected to see that same anger. Instead, social media comments were more ...

  19. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...mammalian cells, finding xCas9 3.7 displayed about the same editing rate as SpCas9 at an NGG PAM. When they ...Adenine base editor xCas9 3.7-ABE also follows the same pattern, with improved editing at an NGG PAM (69...

  20. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ... this purpose.Learn about the effects of CO2 on sample pH Cell morphology: Shape and size matter You should...contaminated.Learn how Addgene uses barcodes to track samples Keep track of passage numbers Keep an eye on the...
Showing: 221 - 240 of 512 results