We narrowed to 261 results for: uros
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TypeBlog PostPublishedNov. 19, 2015, 3:30 p.m....parents' and grandparents' slow growth period." European Journal of Human Genetics 10.11 (2002): 682-688...
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Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog PostPublishedJuly 23, 2015, 2:30 p.m....final target decisions, we intend to focus on motor neuron knockouts so that we can perturb factors related... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog PostPublishedNov. 5, 2024, 8:32 p.m..... (2002). Preferential transformation of human neuronal cells by human adenoviruses and the origin of ... -
Viral Vectors 101: Producing Your rAAV
TypeBlog PostPublishedJuly 16, 2024, 1:15 p.m.... of gene expression in the CNS. Molecular Neurodegeneration, 15(1), Article 1. https://doi.org/10.1186... -
Of Myc and Men
TypeBlog PostPublishedJan. 19, 2023, 2:15 p.m....tagging experiment involved a peptide from the neuropeptide substance P. This protein had a highly specific... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog PostPublishedDec. 10, 2020, 1 p.m.... https://doi.org/10.1038/srep05105 Stephens CJ, Lauron EJ, Kashentseva E, Lu ZH, Yokoyama WM, Curiel DT... -
Plasmids 101: Multicistronic Vectors
TypeBlog PostPublishedSept. 9, 2014, 8:20 p.m....pMSCV-IRES-YFP II IRES Retroviral pCMMP-MCS-IRES-Puro IRES Retroviral pEF1a-IRES-Neo IRES Mammalian ... -
Course-Based Undergraduate Research Experiences (CUREs)
TypeBlog PostPublishedFeb. 9, 2023, 2:15 p.m....spanning chemical biology, biochemistry, and neurobiology. CBE Life Sciences Education. 15(4):ar55. Ma... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog PostPublishedAug. 9, 2016, 2:30 p.m....metastasized tumor cells, or alzheimers-affected neurons, or [your cell line of interest], is the foundation... -
Lentiviral Vector Uses and Overview
TypeBlog PostPublishedMay 19, 2016, 2:30 p.m....experimental use. In 1996, Naldini et al. transduced rat neurons in vivo with beta-gal and other reporters, and ... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog PostPublishedOct. 22, 2015, 2:30 p.m.... to reduce false negatives and produce a more vigourous screen is to use a number of different bait and... -
The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog PostPublishedSept. 3, 2015, 2:30 p.m....aware that “organizing the fun” sounds a little neurotic, but hey- sometimes fun needs to be had in an ... -
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog PostPublishedDec. 2, 2014, 7:23 p.m....the US (New York University School of Medicine), Europe (Basel Institute for Immunology), and Japan (University... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog PostPublishedFeb. 17, 2022, 2 p.m....gutter or the bottom of Tokyo Bay. When a weird, sulfurous smelling, black pellet was sent to the AbuGoot... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog PostPublishedSept. 16, 2020, 1 p.m.... https://doi.org/10.1038/nature14299 Senís E, Fatouros C, Große S, Wiedtke E, Niopek D, Mueller A-K, ... -
Hot Plasmids - August 2020
TypeBlog PostPublishedAug. 18, 2020, 1:15 p.m....optogenetic AAV for expression in forebrain GABA-ergic interneurons. These include mDlx-GFP, hDlx-FLEX-GFP, hDlx-Flex-dTomato... -
Antibodies 101: Reading a Flow Plot
TypeBlog PostPublishedFeb. 6, 2024, 3:13 p.m....Moya-Quiles, M., Minguela Puras, A., Pons, J. A., & Muro, M. (2018). In vitro intracellular IFNγ, IL-17 and... -
Save Time with Transient Plant Leaf Transformations
TypeBlog PostPublishedJuly 25, 2019, 12:03 p.m....reporter gene examples are below. The GUS gene (β-glucuronidase) with histochemical GUS-staining is a very useful... -
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog PostPublishedMay 2, 2019, 12:41 p.m....the genetic basis of evolution in immunology, neurobiology, and more. Prochlorococcus (Penny Chisholm) ... -
Plasmids 101: Gateway Cloning
TypeBlog PostPublishedJan. 12, 2017, 3:30 p.m....expression, we could use a vector like pLenti CMV Puro DEST (w118-1) or the doxycycline-inducible pLIX_...