We narrowed to 855 results for: NTS

...for high-quality antibodies and reagents that work for our experiments!)     Figure 1: The IBEX...their successes (recommended reagents) and failures (not recommended reagents) along with information critical...tissue preservation method, and detergents that they used in their experiments. Researchers can then visit...interested in having high-quality antibodies and reagents that you know work…preferably before you start...dataset currently includes 1049 community-validated reagents from 20 contributors, including 61 fluorescent...works and in what doesn’t, and feedback are vital components of building both the community and the database...Community’s vision is an open, global repository for reagents, protocols, panels, publications, software, and...

... a library of Arch mutants screening for improved brightness. The brightest mutants were then further ...from the past year? Let us know about it in the comments or shoot us an e-mail at [email protected] and ...Cas9(N)-2xNLS) and c-terminal (Cas9(C)-2xNLS) fragments facilitates target DNA cleavage upon co-expression... of specific genes using dCas9-VP64 activator fragments (dCas9(C)-FKBP-2xNLS-VP64 and dCas9(N)-FRB-NES... of recording permanent "memories" of molecular events. These memories would be recorded in the cell's...the relationships between pH changes and cellular events. To overcome the issues with fluorescent pH sensors... tools for spatial and temporal control of cell events Three years ago, Brian Kuhlman’s lab designed photoswitchable...

... - the tendency for students to be more interested in studying animals than plants, and biology teachers...many benefits. Engaging students in active learning through hands-on experiments allows them to develop...conduct the experiments outlined in ABRC’s kits vary by module. As a general guideline, experiments that include...concepts than can be gained from lectures alone. Using plants as an experimental system comes with fewer ethical...include growing plants require pots, trays, soil, fertilizer, seeds and a growth space with fluorescent...fluorescent lights. Other experiments require only seeds, petri dishes, and filter paper. Another benefit of using...questions about growing conditions, to guiding students through specific protocols, ABRC staff are available...

...data with you. “Affinity reagents are among the most commonly used reagents in neuroscience research ...to introduce affinity reagents in protein format within the next year. NIH grants and Addgene’s mission...focus of the NABOR platform will be neuroscience reagents and data, the platform can be expanded for any...secrete antibodies in culture and provide renewable reagents with reliable and reproducible production. However...recombinant antibodies as well as other affinity reagents such as single-chain variable fragment (scFvs)...model for the creation and acquisition of affinity reagents and will enable researchers everywhere.” The ...door for a new generation of recombinant affinity reagents. Addgene is looking forward to working with the...

...the PAM Requirements of Different Cas9 variants Learn about CRISPR Pooled Library Experiments Additional...even image your favorite gene. With so many Cas9 reagents available, it can be difficult to decipher which...your experimental target, SpCas9 and its enhanced variants, eSpCas9 and HypaCas9, are great choices. Check...modifications Site-specific deletions and knockouts Some experiments may require the introduction of a mutation at...an expanding number of Cas9s with looser PAM requirements compared to SpCas9. xCas9 has a PAM requirement...Cas9 proteins (such as SpRY) with altered PAM requirements, giving it more flexibility. A consideration...target genes using dCas9-based repressors Early experiments using dCas9 in bacteria demonstrated that targeting...

...LOV-Turbo into TransitID experiments.  Find TransitID plasmids here! These two developments open up new possibilities...vivo. Incorporating reverse transcriptase (RT) variants, PE6a and PE6b are 500–800 bp smaller than previous...PE6d are optimized for dual-AAV systems. These variants use different RT enzymes and performed large edits...such as long or highly structured templates. Cas9 variants PE6e–g often had even higher editing efficiencies...interchangeable set of fusogenic and targeting components to reprogram delivery vehicles like lentiviral...DIRECTED particles where the fusion and targeting components are interchangeable. Image from Strebinger et...demonstrated LOV-Turbo in neurons, in pulse-chase experiments, and more. Find LOV-Turbo plasmids here! Next...

...because when RSpace documents are deposited into the repositories, links in the documents to data in the external...reproducibility RSpace was designed in response to requirements that emerged from intensive engagement with ...relevant to a particular experiment, series of experiments, or project. ‘Small’ data produced by the research...lab servers specifically designed to hold large amounts of data. Although it may be useful to selectively...reproducibility. For example, it’s possible to import Word documents into RSpace, maintaining the original formatting...edited, and it’s possible to link between various documents. A second example is the integration between RSpace... a two-way integration. It’s possible to send documents from RSpace to a selected Slack channel for discussion...

...the game rules and then you let your students play. As your students play, you can observe, answer questions... make our classes more practical and hands-on. Students in these courses get plenty of theory in their... genetic manipulation of human stem cells when students are not even allowed to manipulate prokaryotic...cells? Especially, if you only have 4 hours and 35 students! After a course coordination meeting with some... I didn’t want to face another group of sleepy students at 8 a.m. many of them focused more on social ...therefore started planning a game that would force my students to use concepts learned from previous lectures...win-win strategy. After each team presented, all students had to pick the best strategy and propose a final...

...labor with up and coming immunotherapy treatments. As treatments enter the clinic, physicians and researchers...keep ahead of the disease in patients and developing collaborative treatments will go a long way in combating...macrophages (M1 and M2) that help create welcoming environments for tumor cells spreading to other organs during...to inhibit further cancer growth and subjecting patients to the risk of microbial infection. Chronic inflammation...one of the most commonly used immunotherapeutic agents. Currently the FDA has 12 approved monoclonal antibodies...cells and T cells activated ex vivo are also contestants for cell-based immunotherapy. Using activated... CRISPR-based clinical trial in 18 lung cancer patients. This study used CRISPR gene editing to knockout...

...microenvironment, a tumor’s backstage team. Microenvironments are cellular regions with specialized structures...structures. Tumors are surrounded by microenvironments and what encircles a disease is highly prone to affect...precisely that for breast cancer. What are tumor microenvironments made of?  A tumor’s genesis is divided into...development? Being pathological active regions, microenvironments shape a tumor’s evasiveness, evolution, and...others repress it. Unfortunately, as a whole, microenvironments promote tumor durability instead of repressing... To understand how cancer cells influence microenvironments and vice versa, let’s take a look at cancer...improving cancer cells proliferation.  How do microenvironments affect tumor spread and drug resistance? Although...