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We narrowed to 599 results for: REV;

Showing: 121 - 130 of 599 results

  1. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...In previous posts for our Plasmids 101 series, we examined a number of important plasmid elements – promoters...and the region of DNA between the loxP sites is reversed.   Deletion: If the sites face in the same direction... cassette) upstream of a gene of interest will prevent gene expression in the absence of Cre. In the presence...recombination event in a single system. The Saccharomyces cerevisiae FLP-FRT recombination system is another site-...

  2. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...fluorogen A split fluorescent reporter with rapid and reversible complementation. Tebo AG, Gautier A. Nat Commun...options. While the BiFC interaction is typically irreversible and requires some time for chromophore maturation...maturation, splitFAST displays rapid and reversible complementation. TagBiFC (split HaloTag) provides maximum...important for their interaction. You can read our previous blog post for more on BiFC principles and experimental...

  3. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...low cost and quick point-of-care diagnosis kits (reviewed in Tsang and LaManna, 2020). To see even more ...uses loop-mediated isothermal amplification after reverse transcription (RT-LAMP) and a colorimetric readout...completed in just 45 minutes. The team deposited the reverse transcriptase, protease, and Bst polymerase constructs... this toolkit include ones for expressing MMLV reverse transcriptase and Taq polymerase that can be used...

  4. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ..., you will need to correct it). It’s easy for reviewers to spot when an application is rushed (whether...that you ask a PI or mentor (your supervisor/ a previous supervisor / someone else in the department) to... times and will have useful advice about what reviewers in your area are looking for. Remember though,...for grant success The two most important things reviewers will be looking for in your research proposal ...

  5. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    Published
    July 2, 2020, 12:40 p.m.
    ...broken during cross-link reversal (not shown in diagram). Cross-links are reversed so that DNA fragments ...cells (~5x107). Together, these two requirements prevent the use of CAPTURE with primary cells or rare cell...multiplexed CAPTURE 2.0 largely replicated interactions previously identified with the original CAPTURE method. ...

  6. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    Published
    Jan. 30, 2018, 2:42 p.m.
    ...new managers and I had a “Eureka” moment while reviewing the results of my Myers-Briggs test:  my undergrads...motivation, but he didn’t want to spend hours reviewing papers to piece together the best protocol;  he...associate feedback with end-of-the-year performance review, but feedback should be given throughout the year...References Becoming the boss. Harvard Business Review, January 2007. Three Rules for Managing a Successful...

  7. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ...then dropped to a range in which the forward and reverse primers are stable. At this temperature the primers...can also be used to quantitate RNA levels using reverse transcription-qPCR (RT-qPCR). The first step of...process requires RNA to be converted to cDNA using reverse transcription and the cDNA is subsequently quantified...template is amplified using several forward and reverse primer sets.  Multiple template PCR - multiple...

  8. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ... are voltmeters for cells! In this blog we will review how these indicators work, their general applications...of the membrane, a concern with several of the previously described systems. Several adaptations of this...   Viral Vectors and Voltage Indicators As previously mentioned, cellular voltage is measured at the... Components of the types of voltage indicators reviewed.   References and Resources   References ...

  9. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...Cas13-based RNA editing system would likely be reversible and would avoid genomic off-targets or indels... RNA knockdown. In initial tests, Cas13b from Prevotella sep. P5-125 (PspCas13b) yielded 62.9% average...editing plasmids here! Future directions Building on previous RNA targeting work with Cas13, REPAIR is the first...edit RNA has multiple advantages, including reversibility and use in non-dividing cells. With the expanded...

  10. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...ambitious, that’s because it is. Zimmer, whose previous books focused primarily on fluorescent proteins...racism and sexism in science, problems with peer-reviewed publications and funding, bioethics in the age...unlikely to be surprised by many of Zimmer’s revelations. The issues he discusses have been making the...of his book today--the day you are reading this review--in the current period of rapid flux, the chapter...
Showing: 121 - 130 of 599 results