Skip to main content
Addgene
Showing: 601 - 650 of 698 results

  7. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...types, during certain stages of development, or by making the Cre inducible (such as with tamoxifen or doxycycline...

  8. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...with a one-stop shop for plasmids and vectors, and making them available to all researchers for a reasonable...

  14. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...

  19. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...having an ‘off’ day – you are both more likely to make a mistake and more likely to want to cut a few corners...

  24. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ... it’s time to move ahead with your infection and make your stable cell line. While we’ve all experienced...

  28. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...translated at the ribosome into polypeptide chains that make up the primary structure of proteins. Most proteins...

  31. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...

  32. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...of rAAV production.  Of course, you will want to make sure that your final stocks only contain rAAV. To...

  33. Antibodies 101: Flow Compensation

    Type
    Blog Post
    Published
    May 16, 2024, 1:15 p.m.
    ...will ‘look’ similar on your flow plot. This will make it difficult to properly gate your desired cell ...

  34. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...degron will behave with a new protein, but you can make an informed choice! References and Resources References...

  35. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...and more photostable than fluorescent proteins, making them ideal for single-molecule fluorescence techniques...

  36. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...that they don’t always require needles. If needles make you cringe or your experiment will allow you to ...

  40. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...recommend Phusion or Q5. Speed up your life Rather than making up your PCR mix every time, pre-made aliquots of...

  41. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...started, and 10 more years to obtain FDA approval and make history in the form of Luxturna™. A better vector...

  42. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...domain proteins, they can be expressed in bacteria, making nanobodies a recombinant and renewable anti-IgG...

  43. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...don’t require a PAM sequence at the target locus, making them more flexible than Cas9/Cpf1. Some Cas13 enzymes...

  47. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...process – such as the viral DNA polymerase – it makes sense that vectorization strategies include creating...

  48. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...synaptic transmission, are right around 250 nm, making it difficult to study the inner workings of the...
Showing: 601 - 650 of 698 results