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  1. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ...are there. Make the most of everyone’s time by considering the level of meeting you need. Project Meetings...review the agenda and add anything that is missing.  During the meeting, take notes on the agenda points. At...

  2. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...delegating for success.  Meet regularly before during and after the assignment to discuss goals, expected...the Management for Scientists Series Learn How Volunteering at a Professional Organization Can Help You ...

  4. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...GFP is often used as a reporter and we will be covering this ever-versatile fluorophore in a later post...selection markers are listed below: Name Gene Conferring Resistance Cell Types* Mode of Action** Working...

  6. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...shock-inducible Cre and Cas9 for easy genome engineering in species like C. elegans and Drosophila. Light...  Mary Gearing...

  7. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ...to rely on differences in emission wavelength. Filtering the emission wavelength partially separates the... Mary Gearing...

  8. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...acts through the Gq pathway and promotes neuronal firing. Plasmid Serotype Name Depositor 44361 AAV1...of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...

  9. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ... ChIP is an incredibly useful application for exploring these interactions, and can give valuable insight...and the Three Steps (Crosslinking, Fragmenting, Capturing), if you ask me.  Each of the three steps need...

  10. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ...you are studying a protein that may bind a ligand during your experiment, then you’d want to make sure that... scientists can freeze hybridoma cells before culturing or they can clone the genes from the hybridoma...

  11. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...molecules to either activate or inhibit neuronal firing. As GPCRs are the largest class of signal transducing... and have different effects on neuron activity (firing or inhibition). AAV preps from DREADD plasmids ...

  12. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    Published
    Aug. 20, 2024, 1:15 p.m.
    ...are some good practice tips on adjusting voltage: During data acquisition, make sure that the signal for...upregulation or downregulation are easier to identify by comparing peak positions to the base expression. When compared...

  13. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ...come up with a new solution.  Now, you may be wondering how this all fits in with science. Well, I’ll ...a software product manager does, you might be wondering what the day to day looks like. Check out the ...

  14. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ... called the Resource Identification Initiative. During this project, authors are asked to add an identifier...guidelines for the reporting of laboratory animals during in vivo research and the CARE guidelines for case...

  15. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...however, they tend to be unstable and may be lost during budding. Yeast Centromere plasmids (YCp): These...with yeast, due to high rates of spontaneously occuring resistant mutants and the insensitivity of yeast...

  16. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    Published
    Oct. 1, 2019, 1:21 p.m.
    ...you on LinkedIn, you will lose opportunities and hiring managers will think it is odd. For those planning...any of these people, including and especially the hiring manager. This is considered intrusive and it’s ...

  19. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...purification preserves RNA structure and naturally occurring complexes present in the cell, it can also introduce...events that occur on a short timescale, such as during drug action or cell signaling. Limitations of RaPID...

  20. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...as cereblon (CRBN) or von Hippel-Lindau (VHL) to bring the endogenous degradation machinery into close ...lentiviral plasmids can serve as a rapid means of comparing functionality of N- and C-terminal fusions and...

  21. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...influence behavior. A conference and a breakthrough! During my graduate studies I attended a scientific symposium...inspiration to resolve the technical hurtle of monitoring protein turnover of specific proteins in situ...

  22. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...techniques. Gel filtration and SDS-PAGE under non-denaturing conditions separate proteins based on size. Whether... and the concentration of the protein solution. During the experiment, the solution is diluted, complicating...

  23. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...informing potential for success in a patient-mutation bearing knock-in mouse model for CTD. Congratulations the...questions,” Hébert says. In the spirit of plasmid sharing and open science, Hébert plans on building upon...

  24. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...similar to a Fab fragment but created through engineering instead of cleavage. They are comprised of the...stable up to 80°C and can refold correctly after denaturing.  Designed ankyrin repeat proteins, or DARPins...

  25. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    Published
    April 20, 2023, 4 p.m.
    ... could present something coherent or to quickly string together all the words that I could remember related...conferences, or even just provide quick updates during a company meeting increases visibility of your ...

  26. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...them for free. Plasmids ship out 2-3 days after ordering, making it a toss-up as to whether it’s faster...cloning method Find blog posts about scientific sharing here Download the Plasmids 101 eBook Resources...

  27. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...spectral properties of the fluorophores you are considering to make sure that they’ll be compatible with ...target. I love FPbase’s Spectra Viewer tool for comparing different fluorophores. We also touched on fluorophores...

  28. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...enough that there is virtually no chance of it occurring randomly. Therefore, inserting loxP sites at deliberate...are active only in certain cell or tissue types, during certain stages of development, or by making the...

  29. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...CRISPR is a simple and versatile tool for genome engineering, but its utility is dependent on its ability ...Nanoblades editing efficiencies of primary cells. Comparing Nanoblades to Existing CRISPR Delivery Methods...

  31. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...specific locations and give rise to progenitors during growth and development or that blood flows through.... While the EnvA genetic sequences are supplied during production of EnvA-pseudotyped RABV, these sequences...

  32. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...hold great promise for improving or potentially curing human diseases that were previously thought to ...as gene delivery tools: Unlike most naturally occurring viruses used in clinical trials, wild type AAV...

  34. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...options are available to you, it may be worth considering an antibody raised in a different species.    ... strategically plan primary/secondary antibody pairing such that each target has a unique species or isotype...

  35. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ...spread. They can be inherited by daughter cells during cellular reproduction, similar to the host’s chromosome...metabolic pathways. Fertility (F) plasmids: microbial sharing F plasmids contain the genes for bacteria to form...

  36. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ... of Research (FoR) symposia held across the USA during this time have revealed that young scientists are...Biochemistry and Molecular Biology/ FoR Hack Day during which several projects were initiated. Recent developments...

  37. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...and Ghyselinck NB. A directional strategy for monitoring Cre-mediated recombination at the cellular level...Addgene Blog  Read about Other Means of Genome Engineering Using FLEx AAV Vectors? Learn about Viral Vector...

  38. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...change (facilitated by the flexible linker) that brings the fluorescent units of the FRET pair together... and diabetes mortality determined by nutrition during parents' and grandparents' slow growth period."...

  40. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...mouse knockout lines should increase. New genome engineering tools also offer hope for the creation of new...knockout animals or cell lines, please consider sharing them by depositing with Addgene! Figures were ...

  42. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...expression baseline. Copy number is then determined by comparing results to a standard curve. For CNV assays, it...gives an absolute value of DNA concentration. By comparing the number of positive droplets for the gene of...

  43. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...to dicot species. In addition, they generated offspring tomato plants that inherited the genomic edit ...If they do (or if they don't!), please consider sharing your experience with other researchers by contributing...

  44. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...the Challenges of Lentivirus Production Tips for Titering Your Lentivirus Preps More resources on Addgene.org...recombinant proteins and viral vectors. Frontiers in Bioengineering and Biotechnology, 9. https://doi.org/10.3389...

  46. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...their mental model of the protein. At some point during this interrogation process, they will almost invariably...recognize a PAM sequence, allow the complementary base pairing between the guide RNA and the target DNA strand...

  47. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...and protein turnover rates. Additionally, by engineering FP fusions associated with cellular organelles...Cas Plasmids - Protein Tagging. Beyond simply monitoring protein localization and activity, CRISPR tagging...

  49. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...similar resource for those involved in monocot engineering projects. The MoClo tools will work in any dicot...think plant biology is massively underfunded considering how important it is. I would really like to reach...

  50. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...to get into the same cell. For simple cloning requiring just one or two recombination events, homemade...DNA polymerase This will minimize random errors during PCR: we recommend Phusion or Q5. Speed up your ...
Showing: 601 - 650 of 832 results