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We narrowed to 792 results for: Arch

Showing: 241 - 260 of 792 results
  1. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...team effort and many late nights, not unlike most research, but the urgent need for the test did catalyze...yet never hesitated to reach out to the greater research community when we needed additional expertise....rapid sharing of these tools with the Zika virus research community. Find the Zika Sensor Constructs at ... of Pharmacy at the University of Toronto. His research is focused on developing cell-free synthetic biology...Arizona State University. His group carries out research in synthetic biology, nanomaterials science, and...
  2. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ... technical challenges they can present. Many researchers find themselves plagued by troubleshooting a ...sensitive to freeze thaw cycles. Depending on the researcher and the type of virus, there have been varied...virus will be used in a couple of days, some researchers prefer to store freshly harvested virus at 4 ...transport and delivery." Japanese journal of cancer research 93.5 (2002): 598-603. PubMed PMID: 12036457. 6...transfected DNA into normal human cells."Nucleic acids research 18.9 (1990): 2733-2738. PubMed PMID: 2339059. ...
  3. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type
    Blog Post
    Published
    Oct. 30, 2018, 1:04 p.m.
    ...fertilization treatments and donated afterwards for ESC research purposes, with the informed consent of the donor...and adult stem cells can be used for different research and therapeutic purposes. Both types have their...skirts issues surrounding the use of embryos in research. With all these benefits, iPSCs have increasingly...increasingly become the ideal experimental system for research in regenerative medicine over the last decade....Richards, History and perspective of stem cell research. Best Pract Res Clin Obstet Gynaecol, 2004. 18...
  4. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...molecular biology in our Plasmids 101 eBook! Researchers use various techniques to control protein expression..., biotechnological, and medical applications. Researches can visualize proteins in vivo by tagging them... also be purified for use in molecular biology research (eg. polymerases and other enzymes might be purified...and biochemistry. When using S. cerevisiae, researchers often place recombinant proteins under the control...Resources on Addgene.org Bacterial expression systems Search for plasmids by expression system ...
  5. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...continue our mission of accelerating scientific research. To make sure that adeno-associated viral (AAV...impurities are generally considered innocuous for research-grade vectors, we’d like to make sure they are...reference sequence. Second, we perform a megaBLAST search on all the reads that did not map to the reference...clinical-grade materials, and is not commonly performed for research-grade materials. Addgene is going the extra mile...that you receive the best AAV vectors for your research. Please use the comments section below to let ...
  6. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...biosensors kit Antibodies for neurodegeneration research   Optimized prime editing in dicots By Emily...New recombinant antibodies for neurodegeneration research   By Ashley Waldron We recently added two antibodies...against important targets for neurodegeneration research: Anti-MAPT [AT8] and Anti-APP [6E10-A5]. Both ...hope these will become valuable tools for your research! If they do (or if they don't!), please consider...consider sharing your experience with other researchers by contributing to the Addgene Antibody Data Hub.  ...
  7. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    Published
    Sept. 23, 2014, 3:05 p.m.
    ... my introduction to protocols.io, I decided to research what other online (and mobile) tools are available...Protocol Online that you might find useful for your research. LabGuru Website: http://www.labguru.com/ Labguru...inventory, managing orders, tracking students and research projects, electronic lab notebooks and protocol...protocols to the protocols.io app so that all of our researchers can easily take those protocols to the lab bench...Data Freedom: The Expansion of Data Sharing in Research Publications by Jim Woodgett        ...
  8. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...a strategy suited to their research. Find Plasmids for Stem Cell Research Delivery Method Mechanism...layers. Choosing a reprogramming method Most iPSC research falls into two categories: 1) studies focused ...at least one method that can help you with your research goals. Are you a new or experienced reprogrammer...The promise of induced pluripotent stem cells in research and therapy." Nature 481, no. 7381 (2012): 295... Cells Check Out Our Interview with Stem Cell Researcher Darrell Kotton Four Factors that Differentiate...
  9. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...acknowledge that negative data is something every researcher experiences and that it often provides incredibly...and the CLOVER Center, each one submitted by researchers like you. Reports are reviewed by Addgene scientists...regions and cell types. Power in numbers A Pubmed search for “AAV” and “brain” over the past decade gives...., McGuire, A. L., & Lázaro-Muñoz, G. (2020). Researcher Perspectives on Data Sharing in Deep Brain Stimulation...Data Freedom: The Expansion of Data Sharing in Research Publications...
  10. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...selection. In a subcutaneous cancer model, the researchers showed that after 24 hours, the unstabilized ...in nature, these systems have been adapted by researchers for many uses when plasmid retention without ...for long-term experiments. They’ve also helped researchers design and optimize cloning methodologies using...genetic modification of Pichia pastoris." FEMS yeast research 9.4 (2009): 600-609. PubMed PMID: 19416369. Kulakauskas....org Check out our molecular biology reference Search for a plasmid here Find plasmids from our special...
  11. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ...types in the sample. The visualization helps the researcher see which of the multiplexed sgRNA was involved...browser’s Control+F or Command+F functionality to search for a particular sequence or name. You can perform...educating and supporting novice to expert-level researchers in achieving consistently successful CRISPR experiments...enthusiast, and when she is not guiding CRISPR researchers, she's either day-dreaming about exploring sci-fi...our CRISPR guide Find CRISPR plasmids for your research Find other CRISPR software resources ...
  12. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...guest bloggers Marcelle Tuttle and Alex Chavez, researchers at the Wyss Institute for Biologically Inspired...domain (Fig 1). Two ways to use CRISPRa for your research CRISPRa for specific gene targets CRISPR activation...scale of activation allows for exploratory-based research as well. For example, CRISPRa can be used to find...medical student at Tufts University and former researcher at the Wyss Institute for Biologically Inspired...Zetsche B, Volz SE, Zhang F (2015) A split-Cas9 architecture for inducible genome editing and transcription...
  13. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ... in Biomedical Research from Albert Einstein College of Medicine. Her doctoral research was conducted ...Figure 1). When behaving properly, FPs enable researchers to investigate the localization and dynamics ...co-mentorship Blossom Damania and Jack Griffith, her research focuses on the replication machinery of Kaposi...secretory pathway."Biochemical and biophysical research communications 430.3 (2013): 1114-1119. Pubmed...
  14. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    Published
    March 5, 2019, 1:08 p.m.
    ...exist for a variety of species, cell types, and research applications. Labeling neurons with Brainbow The... used alongside common, existing tools for fly research that use Flp recombination. In addition to the...small population size of CFP++ cells permitted researchers to longitudinally map the turnover of Langerhans...such as the brain, transparent so that the 3D architecture of a fluorescent neuron is visible. Chemical...more details, check out this blog post from the researchers who developed Tetbow. Conclusion Stochastic multicolor...
  15. The Advances Behind The World's First Personalized CRISPR Treatment

    Type
    Blog Post
    Published
    Sept. 16, 2025, 1:15 p.m.
    ...transplant — creating intense time pressure for the research team. Ultimately, the lifesaving effort was only...possible because of a massive collaboration between research hospitals, universities, private companies, and...invention of base editing doesn’t necessarily mean researchers can fix any problematic C or A mutations in the...higher risk of off-target editing and longer genome search times. Using a machine learning approach, the lab...of a collaborative community focused on CRISPR research, and we’re grateful for every scientist who uses...
  16. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...expression or silencing. To alter DNA modifications, researchers used blunt tools like histone deacetylases, but...epigenetic modifications at a user-specified locus. Researchers showed that TALE-TET1 constructs, which fused...transcription at CpG regions of various promoters. Other researchers additionally fused a TAL effector to LSD1 histone...and dCas9-based epigenome editing. Nucleic Acids Research 47:e23–e23. https://doi.org/10.1093/nar/gky1286...system for targeted DNA methylation. Nucleic Acids Research 44:5615–5628 . https://doi.org/10.1093/nar/gkw159...
  17. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ... selection marker such as thymidine kinase, a researcher may subsequently kill off any cells that still...later restores the target sequence. This allows researchers to avoid making unnecessary base pair changes...powerful technology that may be used for a variety of research applications, including making transgenic cell...molecular and cellular biology and brings 10 years of research experience with stem cells to Transposagen's cell...Mathew applies his specialized knowledge to help researchers identify the right technologies and strategies...
  18. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    Published
    June 13, 2016, 7:08 p.m.
    ...on the right-hand side of the search page to narrow your keyword search to one of these organisms. Help...plasmid kits and other resources for microbial research. Kits, guides, plasmids, and more We have Plasmid...resources that we created filters just for them on our search page. Use the Expression and Insert Species filters...
  19. Addgene’s Blog Gets a New Look!

    Type
    Blog Post
    Published
    Jan. 25, 2021, 5:54 p.m.
    ...you’re new to the topic. Or, try the search bar Now, you can search for the articles you want to find without...on the search bar at the top of the screen and type in what you’re looking for. After you search, you’ll...
  20. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    Published
    Sept. 25, 2018, 2:39 p.m.
    ... us to calculate how much time we have saved researchers. In a recent blog post, we looked at how long... plasmid and how much time Addgene has saved researchers. Assuming 5 hours of hands-on time to make each...are always on the lookout for new ways to help researchers facilitate and accelerate scientific discovery...discovery. How can Addgene help accelerate your research? Let us know! ...
Showing: 241 - 260 of 792 results