...continue our mission of accelerating scientific research. To make sure that adeno-associated viral (AAV...impurities are generally considered innocuous for research-grade vectors, we’d like to make sure they are...reference sequence. Second, we perform a megaBLAST search on all the reads that did not map to the reference...clinical-grade materials, and is not commonly performed for research-grade materials. Addgene is going the extra mile...that you receive the best AAV vectors for your research. Please use the comments section below to let ...

...molecular biology in our Plasmids 101 eBook! Researchers use various techniques to control protein expression..., biotechnological, and medical applications. Researches can visualize proteins in vivo by tagging them... also be purified for use in molecular biology research (eg. polymerases and other enzymes might be purified...and biochemistry. When using S. cerevisiae, researchers often place recombinant proteins under the control...Resources on Addgene.org Bacterial expression systems Search for plasmids by expression system ...

...fertilization treatments and donated afterwards for ESC research purposes, with the informed consent of the donor...and adult stem cells can be used for different research and therapeutic purposes. Both types have their...skirts issues surrounding the use of embryos in research. With all these benefits, iPSCs have increasingly...increasingly become the ideal experimental system for research in regenerative medicine over the last decade....Richards, History and perspective of stem cell research. Best Pract Res Clin Obstet Gynaecol, 2004. 18...

...biosensors kit Antibodies for neurodegeneration research   Optimized prime editing in dicots By Emily...New recombinant antibodies for neurodegeneration research   By Ashley Waldron We recently added two antibodies...against important targets for neurodegeneration research: Anti-MAPT [AT8] and Anti-APP [6E10-A5]. Both ...hope these will become valuable tools for your research! If they do (or if they don't!), please consider...consider sharing your experience with other researchers by contributing to the Addgene Antibody Data Hub.  ...

...a strategy suited to their research. Find Plasmids for Stem Cell Research Delivery Method Mechanism...layers. Choosing a reprogramming method Most iPSC research falls into two categories: 1) studies focused ...at least one method that can help you with your research goals. Are you a new or experienced reprogrammer...The promise of induced pluripotent stem cells in research and therapy." Nature 481, no. 7381 (2012): 295... Cells Check Out Our Interview with Stem Cell Researcher Darrell Kotton Four Factors that Differentiate...

...acknowledge that negative data is something every researcher experiences and that it often provides incredibly...and the CLOVER Center, each one submitted by researchers like you. Reports are reviewed by Addgene scientists...regions and cell types. Power in numbers A Pubmed search for “AAV” and “brain” over the past decade gives...., McGuire, A. L., & Lázaro-Muñoz, G. (2020). Researcher Perspectives on Data Sharing in Deep Brain Stimulation...Data Freedom: The Expansion of Data Sharing in Research Publications...

...types in the sample. The visualization helps the researcher see which of the multiplexed sgRNA was involved...browser’s Control+F or Command+F functionality to search for a particular sequence or name. You can perform...educating and supporting novice to expert-level researchers in achieving consistently successful CRISPR experiments...enthusiast, and when she is not guiding CRISPR researchers, she's either day-dreaming about exploring sci-fi...our CRISPR guide Find CRISPR plasmids for your research Find other CRISPR software resources ...

...selection. In a subcutaneous cancer model, the researchers showed that after 24 hours, the unstabilized ...in nature, these systems have been adapted by researchers for many uses when plasmid retention without ...for long-term experiments. They’ve also helped researchers design and optimize cloning methodologies using...genetic modification of Pichia pastoris." FEMS yeast research 9.4 (2009): 600-609. PubMed PMID: 19416369. Kulakauskas....org Check out our molecular biology reference Search for a plasmid here Find plasmids from our special...

...guest bloggers Marcelle Tuttle and Alex Chavez, researchers at the Wyss Institute for Biologically Inspired...domain (Fig 1). Two ways to use CRISPRa for your research CRISPRa for specific gene targets CRISPR activation...scale of activation allows for exploratory-based research as well. For example, CRISPRa can be used to find...medical student at Tufts University and former researcher at the Wyss Institute for Biologically Inspired...Zetsche B, Volz SE, Zhang F (2015) A split-Cas9 architecture for inducible genome editing and transcription...

... in Biomedical Research from Albert Einstein College of Medicine. Her doctoral research was conducted ...Figure 1). When behaving properly, FPs enable researchers to investigate the localization and dynamics ...co-mentorship Blossom Damania and Jack Griffith, her research focuses on the replication machinery of Kaposi...secretory pathway."Biochemical and biophysical research communications 430.3 (2013): 1114-1119. Pubmed...

...exist for a variety of species, cell types, and research applications. Labeling neurons with Brainbow The... used alongside common, existing tools for fly research that use Flp recombination. In addition to the...small population size of CFP++ cells permitted researchers to longitudinally map the turnover of Langerhans...such as the brain, transparent so that the 3D architecture of a fluorescent neuron is visible. Chemical...more details, check out this blog post from the researchers who developed Tetbow. Conclusion Stochastic multicolor...

...expression or silencing. To alter DNA modifications, researchers used blunt tools like histone deacetylases, but...epigenetic modifications at a user-specified locus. Researchers showed that TALE-TET1 constructs, which fused...transcription at CpG regions of various promoters. Other researchers additionally fused a TAL effector to LSD1 histone...and dCas9-based epigenome editing. Nucleic Acids Research 47:e23–e23. https://doi.org/10.1093/nar/gky1286...system for targeted DNA methylation. Nucleic Acids Research 44:5615–5628 . https://doi.org/10.1093/nar/gkw159...

... selection marker such as thymidine kinase, a researcher may subsequently kill off any cells that still...later restores the target sequence. This allows researchers to avoid making unnecessary base pair changes...powerful technology that may be used for a variety of research applications, including making transgenic cell...molecular and cellular biology and brings 10 years of research experience with stem cells to Transposagen's cell...Mathew applies his specialized knowledge to help researchers identify the right technologies and strategies...

...on the right-hand side of the search page to narrow your keyword search to one of these organisms. Help...plasmid kits and other resources for microbial research. Kits, guides, plasmids, and more We have Plasmid...resources that we created filters just for them on our search page. Use the Expression and Insert Species filters...

...continuation of our efforts to provide resources to researchers in developing countries or that work in under-funded...continued opportunity to get plasmids to the researchers who need them the most. Check back here to learn...more about future awardees and their inspiring research. Additional Resources on the Addgene Blog Learn...Resources Learn About Our Grants to Rare Disease Research Resources on Addgene.org Check out our Cancer...

...you’re new to the topic. Or, try the search bar Now, you can search for the articles you want to find without...on the search bar at the top of the screen and type in what you’re looking for. After you search, you’ll...

... us to calculate how much time we have saved researchers. In a recent blog post, we looked at how long... plasmid and how much time Addgene has saved researchers. Assuming 5 hours of hands-on time to make each...are always on the lookout for new ways to help researchers facilitate and accelerate scientific discovery...discovery. How can Addgene help accelerate your research? Let us know! ...

... Mentor Learn How the Future of Research Looks to Improve the Research Process  Browse our Career Posts...Plasmids Find Plasmids for Your Research ... research project...

...important. Antibodies are a critical component of the researcher toolkit due to their ability to bind to molecular...culprits in the reproducibility crisis of biomedical research (Baker, 2015; Bradbury & Plükthorn, 2015).  To...which represent five experimental strategies researchers can use to show that their antibody performs ...Reproducibility: Standardize antibodies used in research. Nature 518:27–9. https://doi.org/10.1038/518027a...finding and validating suitable antibodies for research. Mabs 8:27–36. https://doi.org/10.1080/19420862.2015.1100787...

... and cloned by Neville Sanjana. This fantastic research tool has been requested from the repository nearly...over a year and a half, a small sampling of the research it has already been used for includes work probing...plasmids being distributed to and accelerating research in labs all around the world. We thank our many...depositors for making these reagents available to the research community through Addgene and look forward to ...