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We narrowed to 588 results for: BASE

Showing: 241 - 260 of 588 results
  1. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ...alternative careers. Science Careers has developed a web-based, career-planning platform called the Individual ...to learn about a variety of science professions based on their skills and interests. Once scientists identify...
  2. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...found in my tissue.” It is based on the same principles as other antibody-based assays, like an ELISA or...cases, a whole organism. But, as with any antibody-based technique, validation and proper experimental design...different companies, such as Validated Antibody Database and CiteAb. The antibody you choose can make or...
  3. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...long-term (AAV). If you perform a lipid-based or electrical-based transfection, most of the nucleic acid...
  4. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    Published
    July 17, 2018, 12:23 p.m.
    ...should be in real scientific practice, so it is based on a win-win strategy. After each team presented...developed to teach gamma-retrovirus or lentivirus-based ex-vivo gene therapy’ strategies to undergraduates...
  5. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...interaction approaches that of GST-glutathione. Biotin-based immobilization for kinetic analysis Proteolytic ...bacteria. Our initial attempt to create a plasmid based on the biotin ligase BirA backbone (Eric Campeau...
  6. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ... remotely can be more difficult if your work is based in the lab. If you’re unsure what to do during this...submitting a registered report, where peer review is based on the methods, not the results. Also consider using...
  7. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    Published
    Jan. 24, 2019, 2:24 p.m.
    ...discriminating between neurons for light microscopy-based neuronal reconstruction. To achieve stochastic multicolor...conventional transgenic strategies. In such vector-based gene delivery systems, the stochastic expression...
  8. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...Topoisomerase based cloning (TOPO cloning) is a DNA cloning method that does not use restriction enzymes...polymerase has an error rate of about 1 in 3,500 bases. Typically polymerases with proofreading functionality... relies on the basic ability of complementary basepairs adenine (A) and thymine (T) to hybridize and form...
  9. Students Invented a New Diagnostic for Lyme Disease — and a Tool for CRISPR Researchers

    Type
    Blog Post
    Published
    May 12, 2026, 1:15 p.m.
    ... Most people are diagnosed with Lyme disease based on their symptoms and history of exposure to ticks...before we finally saw a clean band at around 200 base pairs, which is exactly where we expected it. Aptamer...Chen, J. S., & Chiu, C. Y. (2020). CRISPR–Cas12-based detection of SARS-CoV-2. Nature Biotechnology, 38...
  10. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...They also produce more than 20 other MTRIA sensors based on GPCRs recognizing various neurotransmitters, ...powerful high-throughput screen to select variants based on performance metrics specifically for two-photon...phosphorylated state of an immunoreceptor tyrosinase-based activation motif (ITAM). By fusing ITAM to an RTK...
  11. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...Institute for Protein Innovation (IPI), a Boston-based, nonprofit protein research institute, partnered...unconjugated and recommended for use in flow cytometry. Based on the established applications of the original ...with the Boston Children’s Hospital and Harvard-based lab of Tim Springer, the founder of IPI. Caitlin...
  12. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...with the bulkiness that accompanies mispairing of bases. To reduce off-targets, you’ll want to select a ... Off-targets that come from mutations arise from base/prime editing or Cas cleavage. In the case of Cas...Resources   References Rabinowitz R, Offen D. Single-Base Resolution: Increasing the Specificity of the CRISPR-Cas...
  13. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ... optogenetic tools for manipulating Rho GTPases based on BcLOV4. These single-component systems take advantage...interested in directed-evolution methods, the activity-based selection in mammalian cells used here could be ...Fromme, J. C., & Baskin, J. M. (2023). Activity-based directed evolution of a membrane editor in mammalian...
  14. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ... MACS. But MACS usually only allows for sorting based on a single (lineage) marker, while fluorescence-activated...biorender.com.   Dye stains Dye stains are non-antibody-based stains that bind DNA or free amines of proteins....cytosolic and/or nuclear markers. Lastly, stains, based not on antibodies but on small molecule dyes, are...
  15. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ... The HA frankenbody works just like an antibody-based probe, but is easier and cheaper to generate since...scFvs: Just like antibodies, there are many scFv-based therapies in clinical trials or already in the clinic...blog: Read about frankenbodies, an anti-HA scFv-based probe for fluorescent imaging. Learn more about ...
  16. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ..., especially in in vivo models, and new Cre-lox based technologies are still being developed today. In...Flpbow construct that is functionally similar to Cre-based Brainbow, but is controlled by FLP/FRT recombinase...-44. PubMed. Drosophila Brainbow: a recombinase-based fluorescence labeling technique to subdivide neural...
  17. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ...commonly used for the prediction of T cell epitopes based on the amino acid sequence of the antibody.  NetMHCPan...Assays to Assess Immunogenicity Risk of Antibody-Based Biotherapeutics. PloS one, 11(8), e0159328. https...
Showing: 241 - 260 of 588 results