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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 577 results for: BASE

Showing: 241 - 260 of 577 results

  1. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...Topoisomerase based cloning (TOPO cloning) is a DNA cloning method that does not use restriction enzymes...polymerase has an error rate of about 1 in 3,500 bases. Typically polymerases with proofreading functionality... relies on the basic ability of complementary basepairs adenine (A) and thymine (T) to hybridize and form...

  3. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ... optogenetic tools for manipulating Rho GTPases based on BcLOV4. These single-component systems take advantage...interested in directed-evolution methods, the activity-based selection in mammalian cells used here could be ...Fromme, J. C., & Baskin, J. M. (2023). Activity-based directed evolution of a membrane editor in mammalian...

  4. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...They also produce more than 20 other MTRIA sensors based on GPCRs recognizing various neurotransmitters, ...powerful high-throughput screen to select variants based on performance metrics specifically for two-photon...phosphorylated state of an immunoreceptor tyrosinase-based activation motif (ITAM). By fusing ITAM to an RTK...

  5. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ... MACS. But MACS usually only allows for sorting based on a single (lineage) marker, while fluorescence-activated...biorender.com.   Dye stains Dye stains are non-antibody-based stains that bind DNA or free amines of proteins....cytosolic and/or nuclear markers. Lastly, stains, based not on antibodies but on small molecule dyes, are...

  6. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...Institute for Protein Innovation (IPI), a Boston-based, nonprofit protein research institute, partnered...unconjugated and recommended for use in flow cytometry. Based on the established applications of the original ...with the Boston Children’s Hospital and Harvard-based lab of Tim Springer, the founder of IPI. Caitlin...

  7. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ... The HA frankenbody works just like an antibody-based probe, but is easier and cheaper to generate since...scFvs: Just like antibodies, there are many scFv-based therapies in clinical trials or already in the clinic...blog: Read about frankenbodies, an anti-HA scFv-based probe for fluorescent imaging. Learn more about ...

  8. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...with the bulkiness that accompanies mispairing of bases. To reduce off-targets, you’ll want to select a ... Off-targets that come from mutations arise from base/prime editing or Cas cleavage. In the case of Cas...Resources   References Rabinowitz R, Offen D. Single-Base Resolution: Increasing the Specificity of the CRISPR-Cas...

  9. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ..., especially in in vivo models, and new Cre-lox based technologies are still being developed today. In...Flpbow construct that is functionally similar to Cre-based Brainbow, but is controlled by FLP/FRT recombinase...-44. PubMed. Drosophila Brainbow: a recombinase-based fluorescence labeling technique to subdivide neural...

  11. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...user-defined genomic region. EvolvR, a CRISPR-Cas9 based targeted mutagenesis method developed by the Dueber...changes cytidines or guanines to the other three bases, which limits the level of variation that can be...

  12. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...conduct their hands-on experiments.  As a project-based curriculum, BioBuilder expands learning outcomes...Mary Tamer from BioBuilder! Mary Tamer is a Boston-based writer, editor, and consultant whose work is focused...

  14. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ...to altered function or maybe your favorite FRET-based biosensing technique can be applied to an important...experiments enabled by physical, software, and biology-based tools as well as collaborations between different...

  15. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ...commonly used for the prediction of T cell epitopes based on the amino acid sequence of the antibody.  NetMHCPan...Assays to Assess Immunogenicity Risk of Antibody-Based Biotherapeutics. PloS one, 11(8), e0159328. https...

  16. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...scientists can still reap the benefits of plasmid-based research using synthetic vectors and cultured mammalian..." mammalian ORIs, scientists have usurped viral-based ORIs to fill the void. These ORIs, however, require...

  17. Meet Aavery, Blugene's New Virus Friend!

    Type
    Blog Post
    Published
    Feb. 13, 2020, 2:34 p.m.
    ... more than one person, we selected the finalist based on the first time of submission.)     We love...

  18. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ...by gel or by nanodrop to assemble the  fragments based on their molar ratio. Assembly of the fragments ...you can assemble the different parts of a plasmid based on the cloning strategy you followed. While most...

  20. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...all be ssDNA within the viral particle. The ITRs base pair with each other to allow DNA synthesis of the...57 human adenovirus types, but most vectors are based on adenovirus 5 (Ad5).     Fig. 3 - Viral...
Showing: 241 - 260 of 577 results