We narrowed to 582 results for: BASE;

...interaction approaches that of GST-glutathione. Biotin-based immobilization for kinetic analysis Proteolytic ...bacteria. Our initial attempt to create a plasmid based on the biotin ligase BirA backbone (Eric Campeau...

...discriminating between neurons for light microscopy-based neuronal reconstruction. To achieve stochastic multicolor...conventional transgenic strategies. In such vector-based gene delivery systems, the stochastic expression...

...long-term (AAV). If you perform a lipid-based or electrical-based transfection, most of the nucleic acid...

... remotely can be more difficult if your work is based in the lab. If you’re unsure what to do during this...submitting a registered report, where peer review is based on the methods, not the results. Also consider using...

...Topoisomerase based cloning (TOPO cloning) is a DNA cloning method that does not use restriction enzymes...polymerase has an error rate of about 1 in 3,500 bases. Typically polymerases with proofreading functionality... relies on the basic ability of complementary basepairs adenine (A) and thymine (T) to hybridize and form...

...handy resource for anyone looking to use antibody-based applications in their research.  Like all our eBooks...

... MACS. But MACS usually only allows for sorting based on a single (lineage) marker, while fluorescence-activated...biorender.com.   Dye stains Dye stains are non-antibody-based stains that bind DNA or free amines of proteins....cytosolic and/or nuclear markers. Lastly, stains, based not on antibodies but on small molecule dyes, are...

...They also produce more than 20 other MTRIA sensors based on GPCRs recognizing various neurotransmitters, ...powerful high-throughput screen to select variants based on performance metrics specifically for two-photon...phosphorylated state of an immunoreceptor tyrosinase-based activation motif (ITAM). By fusing ITAM to an RTK...

... The HA frankenbody works just like an antibody-based probe, but is easier and cheaper to generate since...scFvs: Just like antibodies, there are many scFv-based therapies in clinical trials or already in the clinic...blog: Read about frankenbodies, an anti-HA scFv-based probe for fluorescent imaging. Learn more about ...

...Institute for Protein Innovation (IPI), a Boston-based, nonprofit protein research institute, partnered...unconjugated and recommended for use in flow cytometry. Based on the established applications of the original ...with the Boston Children’s Hospital and Harvard-based lab of Tim Springer, the founder of IPI. Caitlin...

...with the bulkiness that accompanies mispairing of bases. To reduce off-targets, you’ll want to select a ... Off-targets that come from mutations arise from base/prime editing or Cas cleavage. In the case of Cas...Resources   References Rabinowitz R, Offen D. Single-Base Resolution: Increasing the Specificity of the CRISPR-Cas...

... optogenetic tools for manipulating Rho GTPases based on BcLOV4. These single-component systems take advantage...interested in directed-evolution methods, the activity-based selection in mammalian cells used here could be ...Fromme, J. C., & Baskin, J. M. (2023). Activity-based directed evolution of a membrane editor in mammalian...

..., especially in in vivo models, and new Cre-lox based technologies are still being developed today. In...Flpbow construct that is functionally similar to Cre-based Brainbow, but is controlled by FLP/FRT recombinase...-44. PubMed. Drosophila Brainbow: a recombinase-based fluorescence labeling technique to subdivide neural...

...conduct their hands-on experiments.  As a project-based curriculum, BioBuilder expands learning outcomes...Mary Tamer from BioBuilder! Mary Tamer is a Boston-based writer, editor, and consultant whose work is focused...

...their choice to share their fluorescent protein based research. This year, we put out another call for...fluorescence-activated cell sorting into four bins based on their eGFP:mCherry ratios. Genomic DNA from each...

...user-defined genomic region. EvolvR, a CRISPR-Cas9 based targeted mutagenesis method developed by the Dueber...changes cytidines or guanines to the other three bases, which limits the level of variation that can be...

...and proud member of Crowdfight. He is currently based in Spain and works on climate change. You can follow...the coordinators at Crowdfight. She is currently based in Spain and works in the field of genetics and ...

...commonly used for the prediction of T cell epitopes based on the amino acid sequence of the antibody.  NetMHCPan...Assays to Assess Immunogenicity Risk of Antibody-Based Biotherapeutics. PloS one, 11(8), e0159328. https...

...to altered function or maybe your favorite FRET-based biosensing technique can be applied to an important...experiments enabled by physical, software, and biology-based tools as well as collaborations between different...

...scientists can still reap the benefits of plasmid-based research using synthetic vectors and cultured mammalian..." mammalian ORIs, scientists have usurped viral-based ORIs to fill the void. These ORIs, however, require...