We narrowed to 322 results for: BRE

1. Making CRISPR Plasmids Using Fragmid

   Type

   Blog Post

   Published

   Aug. 29, 2024, 1:15 p.m.

   ... the Fragmid system, the Fragmid website, which breaks the design process down into four easy steps, provides...

2. Antibodies 101: Stripping and Reprobing Western Blots

   Type

   Blog Post

   Published

   Aug. 1, 2024, 1:15 p.m.

   ...Β-mercaptoethanol (βME) 100–115 mM = 0.7%–0.8% (v/v) Breaks disulfide bonds HCl Adjust pH to 6.7–6.8 ...

3. CRISPR in the Clinic

   Type

   Blog Post

   Published

   Nov. 2, 2023, 1:15 p.m.

   ...doubt that CRISPR/Cas9 technology has been a breakthrough for the genome-editing field and the greater...

4. Engaging with science and society at pgEd

   Type

   Blog Post

   Published

   Sept. 20, 2018, 12:17 p.m.

   ...eugenics and research misconduct. Past ethical breaches such as the Tuskegee syphilis experiment, the ...

5. Hot Plasmids and Viral Preps - January 2021

   Type

   Blog Post

   Published

   Jan. 5, 2021, 2:15 p.m.

   ...Dual-fluorescent DSB Repair) is a double-strand break repair assay for mammalian cells. It's based on ...

6. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

   Type

   Blog Post

   Published

   Sept. 13, 2016, 2:30 p.m.

   ...Howell -Founder of Howell Communications Philip Brenner, PhD - Assistant Professor of Sociology. University...

7. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

   Type

   Blog Post

   Published

   Dec. 1, 2015, 3:30 p.m.

   ... Addgene depositor) Craig Mello. Taking his groundbreaking work in RNAi one step further, his lab is now...

8. The Materials Science of Optogenetics Experiments

   Type

   Blog Post

   Published

   Sept. 17, 2015, 2:30 p.m.

   ...location of your region of stimulation (the z-axis bregma coordinate). Because the light that is emitted ...

9. How-to: 5 Steps to a Great Panel Discussion

   Type

   Blog Post

   Published

   Oct. 22, 2014, 1:57 p.m.

   ... follow and gets things rolling. It can make or break the discussion section. And what about those audience...

10. Polymerase Chain Reaction Overview and Applications

    Type

    Blog Post

    Published

    Aug. 10, 2021, 1:15 p.m.

    ...dsDNA template to a point where the hydrogen bonds break between the base pairs. This results in the separation...

11. Truncated gRNAs for Regulating Gene Expression

    Type

    Blog Post

    Published

    Jan. 10, 2017, 3:37 p.m.

    ...binding the protospacer. The resulting double strand break (DSB) stimulates DNA repair mechanisms that can ...

12. Plasmids 101: 5 factors to help you choose the right cloning method

    Type

    Blog Post

    Published

    Aug. 21, 2018, 12:31 p.m.

    ...methods that make working with multiple inserts a breeze. Among them are some of the homology based methods...

13. Transferable Skills Guide: Managing a Team

    Type

    Blog Post

    Published

    Jan. 30, 2018, 2:42 p.m.

    ...) everything ourselves and it’s a hard habit to break. But as a manager you will be quickly overwhelmed...

14. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type

    Blog Post

    Published

    June 18, 2020, 1:15 p.m.

    ... Vlieger D, Corbett KS, Torres GM, Wang N, Van Breedam W, Roose K, van Schie L, Hoffmann M, Pöhlmann S...

15. Degrading DNA with Cascade-Cas3

    Type

    Blog Post

    Published

    Feb. 11, 2025, 2:15 p.m.

    ...During DNA repair, there is a chance that the two breakpoints will repair without the section between the two...

16. Common Injection Routes in Mice

    Type

    Blog Post

    Published

    Jan. 22, 2025, 9:42 p.m.

    ...patience, as you wait for the anesthetized mice to breathe in the liquid. Restraint: anesthesia Location: ...

17. Twenty Years of Addgene Sharing: CRISPR

    Type

    Blog Post

    Published

    April 18, 2024, 1:15 p.m.

    ...effectors. Nucleases generate a classic double-stranded break, while modulators regulate transcription and chimeric...

18. PhD Applications After COVID

    Type

    Blog Post

    Published

    Jan. 5, 2023, 2:15 p.m.

    ...and video communication. But first, take a deep breath; at least you know you have an answer to that previous...

19. 3D Printing Meets CRISPR Cas9

    Type

    Blog Post

    Published

    Oct. 17, 2017, 12:55 p.m.

    ...sequence of events plays itself out: The researcher breaks eye-contact with any other human in the room. Their...

20. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type

    Blog Post

    Published

    April 19, 2016, 2:30 p.m.

    .... However, if the molecules are confined to a subregion of the cell and/or they cannot tumble in 720°,...