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We narrowed to 662 results for: CLU

Showing: 241 - 260 of 662 results
  1. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...specific blockers, and the manufacturer will usually include recommended blocking conditions on the spec sheet...especially important for highly expressed proteins, including “housekeeping” or ubiquitously expressed proteins... to improve binding and/or reduce background, including trying longer incubations at both temperatures...
  2. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    Published
    Feb. 23, 2023, 2:15 p.m.
    ... Last year, we distributed 165,000 plasmids, including 27,000 CRISPR plasmids and 6,000 biosensors plasmids...antibodies. Everything in this collection, which includes both neuroscience and general-use antibodies, ...
  3. Plasmid Preps: Different Purity, Different Quantities, Different Uses

    Type
    Blog Post
    Published
    June 16, 2020, 1:25 p.m.
    ...other uses.   Cloning grade DNA Molecular cloning includes a set of techniques that are used to insert recombinant...this agent to increase transfection success.  In conclusion, it is important for you to establish in advance...
  4. 6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results

    Type
    Blog Post
    Published
    June 26, 2014, 2:23 p.m.
    ...you run a restriction digest on a gel you always include proper controls like uncut DNA and the proper ladder...amplification reaction (as opposed to using the all inclusive service some companies offer), you can purify ...
  5. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    Published
    Jan. 11, 2017, 8:47 p.m.
    ...engineering research. A variety of new tools came out including the single base editors, Casilio, CombiGEM, and...2A-Puro (PX459) V2.0: What's not in the name? Not included in this plasmid's name are some additional features...
  6. Science Communication Snapshot: DayCon 2015

    Type
    Blog Post
    Published
    June 17, 2015, 9:04 p.m.
    ...organization runs a wide variety of programs, including fall and spring lecture series, science cafes,... information about SITN: DayCon event website (includes archived content) Science in the News website ...
  7. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    Published
    Jan. 23, 2014, 3:23 p.m.
    ...that introduced the kit in December, GreenGate includes the six most frequently used elements in plant...works, just trash it.” Lohmann and his colleagues including Joachim Forner now expect and hope that other ...
  8. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ...on previously described pFA6a-link vectors and include a Kan, SpHIS5, or CaURA3 selection marker. Lee ... variety of genome modifications in S. Pombe, including full and partial gene deletion, overexpression...
  9. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Jan. 23, 2024, 2:15 p.m.
    ...scientists in 88 countries around the world. That included 2008 pooled libraries, 290 antibodies, 18,359 ...it more accessible. Other accessibility updates include a printer-friendly design and alt text on all images...
  10. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    Published
    Dec. 5, 2019, 1:49 p.m.
    ...page, and a lot of the content was either small, cluttered, or hard to find. This made the page look not ... place all of the content above the fold, we decluttered the page elements and allowed our content to ...
  11. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    Published
    Oct. 7, 2014, 3 p.m.
    ...mentoring and applauded the new requirement for PIs to include in their annual NIH progress reports information... research be? The second day of the conference included workshops which were framed in advance by the ...
  12. New Viral Vectors - Fall 2024

    Type
    Blog Post
    Published
    Oct. 3, 2024, 1:15 p.m.
    ...available in the Addgene viral vector repository! This includes the first Addgene prep packaged in the AAV9-X1.1...have a bunch of other new AAV vectors as well — including next-generation biosensors for GABA, dopamine,...
  13. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...biosensors as an example. Many calcium biosensors include GFP fused to calmodulin (CaM) and the chicken myosin... (ER), vacuole or cellular membranes. Examples include blue flame mitochondria-targeted calcium sensor...
  14. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ...Acoustic Reporter Genes (ARGs) are engineered gene clusters that encode proteins that form bacterial gas vesicles...have hollow interiors and a protein shell that excludes water but is gas permeable. These vesicles are...
  15. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...tags, often called polyhistidine tags, typically include six consecutive His residues (or more). Histidine... tags There are many different types of tags, including epitope tags (FLAG, Myc, etc.) and fluorescent...
Showing: 241 - 260 of 662 results