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Showing: 241 - 260 of 650 results

  1. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    Published
    Jan. 23, 2014, 3:23 p.m.
    ...that introduced the kit in December, GreenGate includes the six most frequently used elements in plant...works, just trash it.” Lohmann and his colleagues including Joachim Forner now expect and hope that other ...

  3. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    Published
    May 17, 2016, 2:30 p.m.
    ...over 120 labs have deposited CRISPR reagents, including many gRNA-containing plasmids (McDade et al, 2016...is both sortable and searchable, which can help exclude the data that you are not interested in. You can...

  4. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ...on previously described pFA6a-link vectors and include a Kan, SpHIS5, or CaURA3 selection marker. Lee ... variety of genome modifications in S. Pombe, including full and partial gene deletion, overexpression...

  5. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    Published
    Dec. 5, 2019, 1:49 p.m.
    ...page, and a lot of the content was either small, cluttered, or hard to find. This made the page look not ... place all of the content above the fold, we decluttered the page elements and allowed our content to ...

  6. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Jan. 23, 2024, 2:15 p.m.
    ...scientists in 88 countries around the world. That included 2008 pooled libraries, 290 antibodies, 18,359 ...it more accessible. Other accessibility updates include a printer-friendly design and alt text on all images...

  7. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    Published
    Oct. 7, 2014, 3 p.m.
    ...mentoring and applauded the new requirement for PIs to include in their annual NIH progress reports information... research be? The second day of the conference included workshops which were framed in advance by the ...

  10. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...affected. Previous tools to overcome this restraint include an AAV-based sparse labeling system where a limiting...recombinase can work in other model organisms including mouse and fish, SPARC is likely generalizable ...

  11. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...tags, often called polyhistidine tags, typically include six consecutive His residues (or more). Histidine... tags There are many different types of tags, including epitope tags (FLAG, Myc, etc.) and fluorescent...

  13. New Viral Vectors - Fall 2024

    Type
    Blog Post
    Published
    Oct. 3, 2024, 1:15 p.m.
    ...available in the Addgene viral vector repository! This includes the first Addgene prep packaged in the AAV9-X1.1...have a bunch of other new AAV vectors as well — including next-generation biosensors for GABA, dopamine,...

  14. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ...Acoustic Reporter Genes (ARGs) are engineered gene clusters that encode proteins that form bacterial gas vesicles...have hollow interiors and a protein shell that excludes water but is gas permeable. These vesicles are...

  15. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...biosensors as an example. Many calcium biosensors include GFP fused to calmodulin (CaM) and the chicken myosin... (ER), vacuole or cellular membranes. Examples include blue flame mitochondria-targeted calcium sensor...

  16. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ...members of the ubiquitous activin ligand family, including Activin A. A commercially developed therapeutic...analytical development. His areas of expertise include early stage drug discovery and pre-clinical development...

  17. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...receptors that play many roles in signaling processes including smell, taste, sight, inflammation, and neurotransmission... could be measured using the TRUPATH system. Conclusions Although the importance of GPCR pathways is well...

  18. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ...appreciated and valued This often stems from being included in organizational decision-making. It is imperative...things, diversity of responsibilities, which might include training or teaching others, research, and policy...

  19. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    .... A quick search for "CRISPR", short-hand for Clustered Regularly Interspaced Short Palindromic Repeats...few references for the double nickase system, including one recently from the Zhang group. Learn more ...

  20. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ...technological biological blockbuster known as the CRISPR (clustered regularly interspaced palindromic repeats) Cas...in E. coli in 1987 (1), the concept that these clustered repeat sequences operated as a safeguard against...
Showing: 241 - 260 of 650 results