...are not enough to move the CRISPR field forward. To do that, we need to learn much more about cellular DNA... just stick to RNA? Cas13 may be able to help you do that! How to Design your gRNA for CRISPR Genome Editing...

...in our lab’s environmental impact, but we can all do more to make research more environmentally friendly...recycling program at your institute! What should I do in the lab to help the environment? Well, you could...

...at a peer audience.  Note that lay people can and do consume scientific communication (it is, after all...moves from layperson to expert) and peers can and do consume science communication.  Formal education ...

...call out every Addgene Team because they’re all doing amazing work during this difficult time. One function...distributes and fulfills requests for scientists doing almost every type of biomedical science research...

...So you have this awesome experiment you want to do, but it requires some AAV. You’ve never worked with... but you aren’t going to let that stop you. Where do you start? Turns out like all good experiments, making...

...plasmid to manipulate and use in later work.     How do scientists use plasmids? Generally, scientists use...Some of the many things that plasmids can be used to do include: Produce large amounts of a protein so that...

... time a post doc in the Quake Lab, volunteered to do the sampling and I happily agreed to accompany him...temperature is 30°C. Find IgnaviCas9 here! What can you do with IgnaviCas9? Why would a thermostable Cas9 be...

...Iowa. “I left not really knowing what I wanted to do, but knowing that I wanted to leave the bench.” Her...stumbled upon, about 6 months in, this opportunity to do scientific editing.” It was for Cactus Communications...

...sequence starts with a 'G', you naturally have one and do not need to add an additional 'G'. Q3: What is the...) template, you could then: Confirm your HR by doing Restriction Fragment Length Polymorphism (RFLP) ...

...chemically coloured lymphoid organs did in the past. How do researchers track fluorescently labeled cells? 1.... answer using two-photon microscopy include: How do T cells and/or Neutrophils travel to the sites of...Chtanova et al. 2008, Kamenyeva et al. 2015)? How do blood monocytes patrol blood vessels during immune...Auffray et al. 2007, Finsterbusch et al. 2016)? How do dendritic cells migrate to lymph nodes to activate...

... that is right for you. What type of plasmid prep do you need? As we covered in a previous post on plasmid...midiprep, etc. (more on prep sizes below). But what do we mean by DNA quality? There are a few different...starting culture conditions rather than your kit.   Do you want eco-friendly options? It’s no secret that...  While budget is an important consideration, you do want to make sure you’re getting the best bang for...

...guides as negative controls. Non-targeting guides do not target any site in the genome. This means that...confound the results of high-throughput CRISPR screens. Do short guides (17-18 bp) have less off-target cutting...have fewer off-target effects than long guides, and do not have a major reduction in on-target efficacy....

...train of action potentials that GCaMP6 and jGCaMP7 do not. Most likely, it’s a combination of many factors...supporting data and community engagement required to do this drove Addgene to develop new systems, new partnerships...resources, and collaborations, and will continue to do so, in the hopes that these findings can help others...

...site-directed knock-in. We’ll also discuss what to do if your editing efficiency isn’t as great as you ...insertion or deletion that isn’t a multiple of three will do. A popular screening method for knockouts is Tracking...base pairs modified? In these cases, it’s best to do some planning in the design phase of your experiment...

... resting phase of the cell cycle; quiescent cells do not take up nucleic acid or express transgenes as...economical mode of detection, Uphoff et. al. outline a do it yourself PCR-based approach complete with freely...Learn All About Viral Vector Elements Learn How to Do Genome-Wide Screens with CRISPR Lentiviral Vectors...

...expression levels for most purposes, but what do you do when you’ve tried a common strain and don’t get...popular commercial strains designed to avoid them. Why do I need an expression strain? Protein expression from...

...ends results in the production of blue colonies that do not need to be picked and sequenced for potential...PCR, restriction digest, or sequencing. Pro tips Do not add 5’ phosphates to your PCR primers; you need...

...make, hard to deliver into living systems, and often do not fold properly once inside cells.  Developing ... started with your experiments! What else can you do with the HA frankenbody? Another large advantage ...

...Rooms to divide the audience into small sessions and do a quick break to chat. This can be done randomly ...from academia to industry”. To date, aside from doing science, he has assisted many researchers in gaining...

...individual/non-overlapping cells. Until now, tools for doing this were labor-intensive and required using manipulations...structural deformation, these probes are reversible and do not cause structural deformation or photo-induced...