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Addgene
Showing: 241 - 260 of 529 results

  1. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    Published
    May 1, 2014, 8:23 p.m.
    ...Addgene helps: For Providers: One-time Deposit: Simply deposit with Addgene (it’s FREE!) and we’ll take...

  2. What's New in CRISPR - December 2019

    Type
    Blog Post
    Published
    Dec. 17, 2019, 2:37 p.m.
    ...If you have a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in...Find CRISPR plasmids by function Find recently deposited plasmids ...

  3. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ...selection marker). Additionally, Dr. Formosa has deposited six plasmids with a multiple cloning site in place... complexes. John Pringle and Jürg Bähler have deposited a large collection of plasmids for genome modification...

  5. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    Published
    June 8, 2023, 1:15 p.m.
    ... created the IBEX Knowledge-Base, a community repository and resource for spatial biology enthusiasts....Imaging Community’s vision is an open, global repository for reagents, protocols, panels, publications...

  6. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    ...scientists on the team ensure the deposits we received are what the depositing labs intended. With the use ...

  7. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    Published
    Sept. 29, 2015, 2:30 p.m.
    ... yield during preparation in E. coli. Addgene depositor Indu Maiti has created a new and versatile binary... corn. For both β-glucuronidase (GUS) and GFP reporters, as well as multiple transient and transgenic ...

  8. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    Published
    April 1, 2014, 5:33 p.m.
    ...Investigators (PIs). We've interviewed Addgene depositors who are at various stages in their careers to... to deadlines, writing up clear and attractive reports, being good at presentations and of course, networking...

  9. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    Published
    June 5, 2017, 3:19 p.m.
    ...stock. All of our proceeds go into running the repository and serving scientists better. That includes ...reagents Addgene makes it easy for scientists to deposit and request plasmids (via web interface, processes...

  11. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ...capstone project report and presentation, during the final week at Addgene. This report shared my work ...

  12. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...puromycin, hygromycin) and fluorescent protein reporters (GFP, BFP, RFP, no FP). The vectors each contain...region of the RPL41 gene, to drive the selection/reporter parts of each construct. This new tool kit allows...

  13. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    Published
    Nov. 17, 2016, 3:30 p.m.
    ...money producing virus from select vectors in the repository yourself, you can now order ready-to-use virus...of the most popular lentiviral vectors in the repository are used to produce CRISPR tools, we’re starting...

  14. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    Published
    Sept. 14, 2021, 1:15 p.m.
    ...webpage for any specific recommendations from the depositing lab. If your vector is large (>10 kb) or has ...4847–4851. https://doi.org/10.1073/pnas.77.8.4847 Trepotec Z, Geiger J, Plank C, Aneja MK, Rudolph C (2019...

  16. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    Published
    Sept. 21, 2023, 1:15 p.m.
    ...primary antibody that is directly conjugated to your reporter circumvents the issue. However, directly conjugated...which uses just a primary antibody that has your reporter (in this case a fluorophore) directly attached...

  17. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ...While the vast majority of CRISPR plasmids in our repository use Cas9 to cut DNA, Addgene Science Communications...Resources Learn More about Viral Vectors Browse the Repository  ...

  18. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    ...calcium-binding sites as possible for added sensitivity. As reported in Nature Methods, the FRET changes of these Twitch...magnesium sensor, the first of its kind. “I hope by depositing at Addgene, people will start using them,” Merkx...

  19. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ...lentiviral preps of select lentiviral vectors from the repository for you. We perform test infections with all ...lentiviral format Another popular set of tools in the repository are CRISPR pooled gRNA libraries that can be ...

  20. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ...career exploration site Inventing Heron has a radio reporter challenge, and code.org has coding tutorials for...experiments carefully. Consider submitting a registered report, where peer review is based on the methods, not...
Showing: 241 - 260 of 529 results