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Showing: 241 - 260 of 553 results

  1. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    Published
    July 28, 2017, 1:30 p.m.
    ...Harvard University. In my previous two posts, I’ve described the fundamentals of how to work with Drosophila...Drosophila as an experimental model organism. I then described the Gal4/UAS system used by geneticists to study...manipulations. You can produce some very beautiful immunofluorescent images like in this paper (2). Metabolic assays...

  2. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    Published
    July 6, 2017, 1:59 p.m.
    ...positive effect on cancer treatment (Ref 1). As described in the presentation, starvation or the use of ...can find useful tips on in vivo imaging in our Fluorescent Protein Guide. Throughout the conference aspirin... with T cell receptor engineering (Ref 5)  as described by Blue Flame Addgene depositor Professor Michel...

  3. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type
    Blog Post
    Published
    May 11, 2017, 2:30 p.m.
    ...27478040. The lung cancer mutant collection is described in this paper. Systematic Functional Interrogation...the genetic pathway reference collections are described in this paper Systematic morphological profiling...  The genetic pathway reference collection is described in this publication We also wrote an extensive...

  4. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...biologically relevant modification, or add in fluorescent or chemically reactive side chains, all of which...through a seminal paper from Peter Schultz’s lab describing a technique that allowed for intentional incorporation...of Phosphoserine into Recombinant Proteins in Escherichia coli. 12(21). https://doi.org/10.21769/BioProtoc...

  5. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    Published
    June 6, 2024, 1:50 p.m.
    ...cytometry controls we’ll discuss are single color, fluorescence minus one (FMO), and isotype. You should always...controls like FMO or isotype controls, which we’ll describe a bit later. Single Colors Single color controls...(C) with APC-Cy7.   FMOs FMO stands for fluorescence minus one. It is used as a negative control ...

  6. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ...Cell, Musunuru, Chad Cowan and their colleagues describe a much more efficient tool for doing that disease...people unfamiliar with this new TALEN kit, can you describe it for us? Musunuru: TALENs are a type of genome...including FokI endonuclease domain as well as fluorescent proteins used to sort the cells after the plasmids...

  7. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...either through a chromogenic or chemiluminescent reaction. Chemiluminescence, which is light produced by ...detect your antibodies using techniques like fluorescence microscopy, flow cytometry, or spectrophotometry...

  8. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...specifically refers to the organizational schema described above, and not any particular cloning technique...cellular engineering goal, several of which are described below. Multigene circuits assembled through MoClo...relationship between genotype and phenotype is best described with “it’s complicated.” Applying MoClo Interested...

  9. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...should appear fluorescent if they were infected. While there may be some low-level fluorescent protein or...virus. Alternatively, if the virus encodes a fluorescent protein, the packaging cells could be examined...

  10. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...colorimetric ladder, visible to the naked eye; a fluorescent ladder compatible with your imaging system; or...having to re-run the western.  Remember that chemilumescent methods, like horseradish peroxidase protein...Underexposed Increase exposure time Chemilumenescence: use a more sensitive exposure kit Too...Overexposed blot Shorter exposure time Chemilumenescence: use a less sensitive exposure kit No ...

  11. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ..., if your protein is fused with a fluorescent protein), fluorescence microscopy can theoretically be used...Addgene.org Check Out Our Protocols Pages Browse Fluorescent Protein Plasmids Find Mammalian Expression Vectors...

  12. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...following cloning methodologies have been extensively described in other articles; so if you are interested in... to assemble up to 6 different fragments in a prescribed order. These fragments can be pooled in one tube...homology. You might also consider FastCloning, first described by Chaokun Li and colleagues. Here you simply ...

  13. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...photostable than fluorescent proteins, making them ideal for single-molecule fluorescence techniques. After...used to measure signal along the dendrite. D) Fluorescence signal as a function of the distance between...

  14. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...proteasome for degradation. Like several other tags described here, the dTAG uses a small molecule, a tag, and...for visualization of proteins of interest. A fluorescent Halo ligand can be added to media, buffer, etc...there are antibodies to it and ways to achieve fluorescence with it. AID The auxin-inducible degron (AID...

  15. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...template with the endogenous gene. Here, we'll describe the techniques and the plasmids used to inactivate...Conditional knockouts The methods and plasmids described in the preceding sections are simple ways of knocking...knockout, researchers often use the Cre/Lox system described earlier. In this case, you design your targeting...

  16. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ...organism carrying a synthetic gene drive were to escape the laboratory, the drive could eventually spread...protocols, they can reduce the risk of accidental escape to a negligible level. One major reason we chose...performing experiments in a geographic area where escaped organisms won't be able to find mates2. For example...

  17. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...are easy to trace and not lost in a jumble of fluorescent neurons. A simple but flawed approach to multi-color...constitutively express a red, green, or blue (RGB) fluorescent protein (4a-c). When high doses of this viral...morphology studies when used as part of a two part fluorescent tag-then-express system that allows for controlled...

  18. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ...looking at your resume while they wrote the job description. You check off all the boxes - you have the desired... gaps in scientists' transferrable skills. In describing the limitations of the study, the authors noted...over email. Examine your current and past job descriptions. Scan through all the things for which you are...

  19. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...worked extensively with enChIP systems. Can you describe this technology and its advantages? Fujii: In ...enChIP system using CRISPR technology. Can you describe this technology and some of its potential applications... any other technologies that you would like to describe?  Fujii: I believe that "Biology is Method", i.e...

  20. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...areas associated) compared to an animal running to escape a threat. Locomotion can thus be used as a readout...behaviors are possible: active swimming in attempts to escape, or passive floating on the water surface. The ...that were not (Hare et al., 2019). The research described here is only a small selection of the increasing...
Showing: 241 - 260 of 553 results