We narrowed to 253 results for: IRES
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TypeBlog PostPublishedJuly 20, 2021, 1:15 p.m..... It does not, however, permeabilize cells and requires subsequent permeabilization with detergents such...
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Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog PostPublishedAug. 15, 2017, 1:24 p.m....auto-fluorescence (Drobizhev et al. 2011). But this requires other lasers than the standard Ti:Sapphire laser... -
A Needle in a Base-Stack: Cas9 Structural Biology
TypeBlog PostPublishedJune 4, 2024, 1:15 p.m....weak, random binding to precision gene editing requires three major steps: RNA loading, DNA sequence recognition... -
CRISPR 101: Multiplex Expression of gRNAs
TypeBlog PostPublishedSept. 10, 2020, 11:45 a.m....editing, and a subsequent curing protocol that requires only 2-3 hours incubation. Kondo Lab multiplexed... -
Prime Editing: Adding Precision and Flexibility to CRISPR Editing
TypeBlog PostPublishedJan. 13, 2025, 9:49 p.m....Prime editing is precise and versatile, but it requires careful optimization to achieve the best efficiency... -
Antibody Validation for Flow Cytometry
TypeBlog PostPublishedSept. 10, 2024, 1:15 p.m....antigen of interest in the given protocol. This requires use of one of the five pillars of antibody validation... -
Deep Dive: qPCR
TypeBlog PostPublishedSept. 29, 2022, 1:15 p.m.... practice, multiplexing is quite difficult and requires significant optimization to ensure the results... -
CRISPR 101: Cytosine and Adenine Base Editors
TypeBlog PostPublishedFeb. 13, 2025, 2:15 p.m....does base editing work? Base transition editing requires three elements. They are, broadly: A Cas protein... -
CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog PostPublishedSept. 28, 2020, noon...editing, and a subsequent curing protocol that requires only 2-3 hours incubation. E. coli and T. citrea... -
Troubleshooting and Optimizing a Western Blot
TypeBlog PostPublishedSept. 17, 2024, 1:15 p.m....concentration of your reagent. Be aware that this approach requires excellent attention to detail, since you’ll end... -
Viral Vectors 101: Systemic Capsids
TypeBlog PostPublishedOct. 12, 2023, 1:15 p.m....and astrocytes located in the CNS, however, it requires high doses. Requests have shifted toward the more... -
27 Hot Plasmids from 2016
TypeBlog PostPublishedDec. 22, 2016, 3:03 p.m....for more selective targeting of fusion proteins, requires less biotin, and shows enhanced labeling of proximate... -
28 Hot Plasmid Technologies from 2015
TypeBlog PostPublishedDec. 23, 2015, 3:30 p.m....for a protein of interest (POI). This technique requires the production and expression of fusions between...