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We narrowed to 849 results for: NTS

Showing: 241 - 260 of 849 results

  1. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...integrase mutants in the early 1990s (Leavitt et al., 1996), and since then, numerous variants have been...These trials unfortunately resulted in several patients developing T-cell acute lymphoblastic leukemia...titering IDLVs include detecting vector particle components or performing quantitative PCR titration of vector...research, we wish you the best of luck with any experiments involving IDLVs. And remember: You can never ...Consequences on Host Gene Expression. Viruses: Essential Agents of Life, 147–175. https://doi.org/10.1007/978-94...leukemogenesis following gene therapy of SCID-X1 patients. The Journal of Clinical Investigation, 118(9)... Human immunodeficiency virus type 1 integrase mutants retain in vitro integrase activity yet fail to ...

  2. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...DNA-based reagents are named for soup ingredients! From pSOUP to mCherry, satisfying ingredients reagents...Gram-positive bacteria (Kellenberger et al., 2013). Other variants of Spinach include Baby Spinach, a miniaturized...short non-coding RNAs (Autour et al., 2018). Three variants of Mango, coined Mango II, III, and IV, were then...high binding affinity to TO1-biotin. Two of these variants exhibit increased brightness compared to Mango...useful for both live and fixed cell imaging. New ingredients in the aptamer soup In addition to these established...

  3. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ... and quality for HIV-1 patients. However, ART is not an HIV-1 cure. In patients who stop ART, viral levels... latently infected cells. Although ART-treated patients lack plasma HIV-1, they are at increased risk ... isolated CD4+ T-cells from two HIV-1 infected patients. Although this is an exciting finding, it’s important...important to note that these HIV-1 patients were ART-naive, so this experiment models the active HIV-1... ART-induced viral control found in most HIV-1 patients. Obstacles for CRISPR HIV-1 therapy Both of these...CRISPR gRNAs require DNA sequence-specific binding. Patients’ HIV-1 genomes will need to be sequenced to determine...discouraging, it’s important to note that these experiments were done in cell culture, and the cells infected...

  4. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ... Designs Consulting (EDC), we instead have our clients focus on their experimental needs and consider ...accomplish your science, and can include: equipment, reagents, animals, glassware, and even people. When developing...even includes clocks, timers, and phones.  As for reagents and consumables (such as pipet tips, glassware...to need a tech because you’ll be doing mouse experiments, start defining keywords you’ll want to find ...their goals, rather than focusing on long term investments. For example, purchasing “used” equipment can...resource budget, and know how to decide which components are the most critical to your research, negotiations...limitations. Everything is negotiable. Try asking for discounts, collaborations, access to internal award applications...

  5. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...some extra guidance to understand the specific components that make up a plasmid and why each is important...spend more time developing cleverly designed experiments and innovative solutions necessary for advancing...naturally in archaea and eukaryotes such as yeast and plants. In nature, plasmids provide one or more functional....   Figure 1: Map of a plasmid with its elements described below.   Vector Element Description...as they grow, scientists can easily make large amounts of plasmid to manipulate and use in later work....plasmids can be used to do include: Produce large amounts of a protein so that scientists can purify and ...Plasmids 101 series, please let us know in the comments. Note:Marcy Patrick contributed to the writing...

  6. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ...awareness for rare disease and helps patients find the correct treatments, if available. As part of the team...play with my kids like any other youngster. His parents, Kristi and Rico, had to keep him from running ...triggers appropriate bone regeneration in healthy patients. However, the R206H mutation makes ACVR1 responsive... with inappropriate activin-ACVR1 interactions prevents FOP bone growth in mouse models. As this biologic... But, as we in the scientific community know, treatments that work well in animal models do not always... Disease Researchers Check Out Potentiall HIV Treatments with CRIPSR Resources on Addgene.org Find ACVR1...

  7. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type
    Blog Post
    Published
    Aug. 8, 2019, 1:19 p.m.
    ...to address the comments to “you” rather than “the authors.” By addressing the comments to “you” I can ... figures). After finishing my section-specific comments, I re-read what I wrote about the paper after ...in the first (and clunky) version of my general comments and adjust them if my opinion changed after this...that there is another human who is reading your comments. Receiving a peer-review is already a humbling...young scientists that are reading your peer-review comments will learn from them and propagate this culture...ratio in Arabidopsis, (2) expression patterns in plants with enhanced sodium accumulation in their roots...

  8. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...oligomerization can lead to misleading artefacts in your experiments. Read this post from Erik Snapp, Associate Professor...oligomerization can be and how to avoid it in your experiments. Better Dyeing Through Chemistry & Small Molecule...fluorophores can still be used in a wide variety of experiments. Read more to find chemical fluorophores for ...and/or engineer “temporally ordered biochemical events” such as those that occur during development. Read...study basic virus biology while leaving virulent components behind. Minigenomes are stripped down viral genomes...fuel sources.” In her work, Dr. Polka made R-body mutants that respond to different pH’s and some that are...libraries of CRISPR gRNAs to use for screening experiments. Get the full details on how to make these libraries...

  9. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    ...connection between molecular- and cellular-level events and behavior. It’s a problem that he considers ...change has a particular advantage for observing events taking place inside cells that are on the move,...Methods, the FRET changes of these Twitch sensor variants were optimized by testing various linker configurations...large-scale functional screen in bacteria. Sensor variants were then refined by a secondary screen in rat... than intensity-based readouts.” Griesbeck also points out that the approach he and his colleagues took...“trick” to the development of additional color variants. This resulted in the construction of redCALWY...redCALWY-1, a red-shifted FRET sensor for zinc using variants of mOrange2 and mCherry as donor and acceptor ...

  10. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ...in silico. These algorithms are trained on vast amounts of data to calculate the relative immunogenicity... replicate the complexity of antigen processing events that take place within an antigen-presenting cell...conditions, etc. that can vary widely between recipients.   In vitro prediction of drug immunogenicity... to validate the findings from the in silico assessments, as well as analyze the contributing factors ...in-vitro models can be built that contain various components of the immune system. Figure 2: In-vitro...Potential Use of HLA Haplotypes to Predict Vulnerable Patients. Frontiers in Immunology, 13. https://www.frontiersin.org...Algorithm Infrastructure and a Vision for Future Enhancements. Frontiers in drug discovery, 2, 952326. https...

  11. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...that’s where MTAs, and similar agreements, shine.  These types of agreements allow for centralized tracking...and developments.  MTAs also mean that everyone is on the same page as to how the shared reagents should...organizations to make policy decisions on research use agreements. The UBMTA, which is the MTA most plasmids at...department is responsible for managing research use agreements like MTAs.  Addgene does a lot to ensure that...tracking when biological reagents are shared — both at what time and with whom. Tracking these connections...

  12. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...classes, workshops, book clubs, seminars, and social events. Building a community science lab In the Fall...it. We started out by holding a few journal club events and soon realized that it was easy to find people...entrepreneurs using our space to perform early-stage experiments. For example, Dave Grawoig is using nanopore ...sequencing at BosLab to perform proof-of-concept experiments for discovering novel therapeutics. Dave is a...optimizes plant cell culture and transgenesis he wants to share his knowledge with the community by teaching... will engineer yeast to glow, produce synthetic scents, detect compounds in the environment, and openly...ecology but she became a member at BosLab because she wants to engineer microbes. We’ve ordered a few yeast ...

  13. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

    Type
    Blog Post
    Published
    Nov. 20, 2018, 8:17 p.m.
    ...long-lasting way to identify and reference documents, files, or physical reagents. What are persistent identifiers... there is insufficient information about which reagents were used? Unambiguous identification of a reagent...Object Identifier (DOI). Nearly all the papers or preprints you read or produce have one. The DOI serves as...publications help to enable reproducibility of experiments. Additionally, proper citation of these Addgene...standardized persistent identification of research reagents, several journals or organizations now recommend... the RRID and compact identifier formats. Acknowledgments Many thanks to Daniela Bourges for editing ...

  14. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...cells. Before getting into the mammalian plasmid components, it may be useful to describe the means of introducing... bacterial cells); however, the techniques and reagents vary. Plasmid transfection into mammalian cells...transfection. Unlike bacterial transformation, scientsts do not "select" for cells that have taken up the...transfection and the elusive "mammalian ORI" For many experiments, it is sufficient for the transfected plasmid...plasmid replicates in the cell, as many of these experiments are concluded within 48 h of transfection. Of...void. These ORIs, however, require additional components expressed in trans within the cell for effective...ORIs, respectively. The presence of these viral components greatly reduces the rate of plasmid dilution ...

  15. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ...competitive endeavor. Due to the COVID-19 pandemic, students will face a reduced supply of internships as a... to the opportunities of their dream.  To help students focus on the application process, and simplify...internships for high school, college, and graduate students. In order to gain a competitive advantage this...errors, sentence structures, and overall format (fonts, font size, legibility, and more). Tips on drafting...leadership status in their current field of expertise augments their authoritative voice. Send a draft of your...in the Chemical Biology Program at Harvard. Roo wants to simplify access to academic resources among economically...outreach among low-income communities to educate parents about resources that can help their children thrive...

  16. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...their required components are very similar. While plasmids containing virus-specific elements such as the...or infrequently cycling cells but is fine for experiments with immortalized cell line cultures. Lentivirus...between the two systems, other, more general, viral components such as a heterologous envelope or a post-transcriptional...whole animal administration, such as in mouse experiments. AAV also has multiple serotypes (12 naturally...strategies may need to be considered if size constraints are an issue. Adenovirus Adenovirus has a linear...Addgene blog Viral Vectors 101: Viral Vector Elements Viral Vectors 101: An Introduction to AAV Viral...

  17. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ... but should contain a table of contents that lays out which experiments can be found in each folder. This...their results. We are adept at recording wet-lab experiments using a “traditional” lab notebook; however, ...the future. Stay organized Set up a table of contents in the top directory: A directory is a file system...document with a brief description of the directory contents, at a minimum. This may or may not be where you...in tact! Take notes Comment in your scripts: Comments on what a given line of code does are very useful...Software You Can Use to Save Time and Track Your Experiments Try out the CRISPR Software Matchmaker Additional...

  18. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...Before you jump straight into the exciting new experiments that you’ve been planning, it is a good idea ...yourself have to perform extensive validation experiments for every antibody that you receive; citing existing...However, it is your responsibility to determine what counts as adequate validation. If you are not convinced...your sleeves and perform some extra validation experiments yourself.  Goal of Antibody Validation  As you...assess existing data or plan your own validation experiments, it’s important to know what you are aiming to...expected in another. You’ll need to validate that points 1 and 2 hold true under your specific experimental... that your new antibodies will work for your experiments. Capture MS Strategies This approach uses immunoprecipitation...

  19. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...professor who once decided that to teach high school students PCR, they first had to understand hydrogen bonds...every project, time, attention, and/or length restraints mean you will have to pick and choose what concepts...when needed. The trick is to communicate your key points well enough that the audience can take that knowledge...Different modes of communication take up different amounts of mental bandwidth - if not exactly cognitive ... audience, ask! They are experts in what their students know.   Ask the learners themselves for feedback... about the questions you’ve received, read the comments, seek feedback from others, and listen quietly...simpler presentation than I had planned; other times students were fired up and enthusiastic and needed a faster...

  20. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...washout experiments (Nabet et al. and Weintraub et al.), chemical and genetic rescue experiments (Nabet... for degrading targets in different cellular compartments, and in vivo utility. dTAG molecules will be... line of interest prior to starting knock-in experiments. Test dTAG molecule toxicity in new cell lines...is recommended, while quantitative proteomics experiments will provide the best confirmation of selective...-substrate degradation with dTAG-13. Example experiments See our recent papers noted below for examples...Nabet et al., Erb et al., Weintraub et al.), assessments of fusion chimera functionality (Nabet et al....
Showing: 241 - 260 of 849 results