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We narrowed to 466 results for: SHI

Showing: 241 - 260 of 466 results
  1. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ...represents the proportion of cells that have either a frameshift or 21+ bp indel. This score is a useful measure...Represents the proportion of cells that have either a frameshift or 21+ bp indel. This score is a useful measure...
  2. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...vectors expressing BDNF in the hippocampus causes a shift towards antidepressant behavior in rats in the forced...neuroscience to reveal insights into the complex relationship between genes, neurons, and behavior. There ...
  3. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...mouse an attractive tool to probe gene-function relationships in defined substructures of the CNS as well ...Rochester Medical Center. He studies gene-function relationships in the CNS using CRISPRa as a member of the ...
  4. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...and how our search for solutions led us to a partnership with Seqera Labs.              DNA sequencing...Creating a bioinformatics workflow in Nextflow Establishing a complete pipeline however, required more than...
  5. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...Antibody here!   Anderson, Z., Zhu, H., Riedel, T., Moshinsky, D., Meijers, R. (2024). Western Blot for Anti-V5...and in ternary complexes with other proteins, establishing CRBNmidi as a promising tool for future structure-guided...
  6. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ... typhimurium, S. ordonez, Serratia marcescens, Shigella spp., Vibrio cholerae, Xanthomonas campestris ...10.1111/1574-6968.12241  Keen, N. T., Tamaki, S., Kobayashi, D., & Trollinger, D. (1988). Improved broad-host-range...
  7. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...difficult to treat in the future. Patients not finishing the whole course of antibiotics prescribed to ... cleanliness and personal hygiene standards. Handwashing is an essential component of preventing infection...
  8. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F (2014) Genome-scale transcriptional...Mammalian Protein Complex Structure, Function, and Membership Using Genome-Scale Fitness Screens. Cell Systems...
  9. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz at the National...of North Carolina at Chapel Hill. Under the co-mentorship Blossom Damania and Jack Griffith, her research...
  10. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...are listed below: EMSA (Electrophoretic Mobility Shift Assays) ELISA (Enzyme-Linked Immunosorbent Assay...Biotinylated probes in the electrophoretic mobility shift assay to examine specific dsDNA, ssDNA or RNA–protein...
  11. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...of a problem when the excitation wavelength is shifted to longer wavelengths. Therefore, the use of bright...25831507 PubMed Central PMCID: PMC4394297. 16. Iwano, Satoshi, et al. "Single-cell bioluminescence imaging of...
  12. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...Multiple uses: From multiplexing to multiscaling NIR-shifted spectrum is perfect for use in spectral multiplexing...autofluorescence, low scattering, and deep penetration of red-shifted light. The same NIR FPs and biosensors can be imaged...
  13. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...year, Russell and Hirata from the University of Washington reported a surprising finding - when delivered...effective for introducing non-sequence specific frameshift mutations through aberrant double strand break...
  14. Transferable Skills Guide: Time Management

    Type
    Blog Post
    Published
    Dec. 5, 2017, 1:52 p.m.
    ...it. The other half of each day you can spend accomplishing small tasks (like... preparing plasmids to send... Send out as many emails as possible While accomplishing any one of these things won’t mark an end to...
  15. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...develop a biosensor for oxytocin, Daisuke Ino and Hiroshi Hibino and colleagues devised a “Modular fluorescence...fluorescent biosensors for monitoring RTK activation by shift in cellular localization. Image adapted from Farahani...
  16. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...to understand. (1-2 sentences). For example: Distinguishing correctly folded and misfolded proteins is ...Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz at the National...
  17. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...approximately 1 in 5000 males. DMD is caused primarily by frameshift mutations in dystrophin, a protein essential ...regions, so low-frequency indels will not cause frameshifts. Figure adapted from Osterout et al. (2015b) ...
  18. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...experimental condition you could look for a slight size shift in the protein band before and after treatment.  ...0003-2697(81)90281-5 Pillai-Kastoori L, Heaton S, Shiflett SD, Roberts AC, Solache A, Schutz-Geschwender ...
  19. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 2:15 p.m.
    ...of AAV1 and AAV2, with only six amino acids distinguishing its capsids from those of AAV1 (Issa et al....N. L., Puschnik, A. S., Davulcu, O., Diep, J., Ishikawa, Y., Jae, L. T., Wosen, J. E., Nagamine, C. M....
Showing: 241 - 260 of 466 results