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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 577 results for: SIM

Showing: 241 - 260 of 577 results

  1. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ... an advanced search option. For the former, you simply type in identifier keywords (Cas name, RefSeq ID...phylogenetic nomenclature and discover orthologs with similar properties (Makarova et al). CasPEDIA is an evolving...

  2. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...organisms. This is often cDNA, shRNA, or something similar, and is contained on the transfer plasmid. If expression...different from gamma-retrovirus, but they perform very similar functions to infiltrate the host cell and deposit...

  3. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...Packaging using 293T cells This method is very similar to lentiviral packaging methods. The three components...constructs. Why do many γ-retroviral plasmids have similar names? The nomenclature of the plasmid backbone...

  4. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...this post in the graphic below - including some simple comparisons. The easy multiplexing afforded by...integrated RNA and CRISPR/Cas toolkit in human cells. Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK. ...

  5. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    Published
    Feb. 18, 2014, 4:22 p.m.
    ...and mentees. Because the participants are at a similar development stage, they have a lot to learn from...Sometimes it is helpful to find peers that share a similar direction or problem. For example, a group of people...

  7. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ...Assembly, Golden Gate etc.), and try to find the simplest way to do it (i.e. avoid assembling too many fragments...help you at this stage and that can be used to simulate in silico cloning. This will definitely help in...

  8. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...experiences. If you’re hearing multiple people with similar misconceptions, it’s time to rework your strategy...group was just really tired and needed a slower, simpler presentation than I had planned; other times students...

  9. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...variables (scFvs) are ~27kD engineered fusion proteins similar to a Fab fragment but created through engineering...different antigen; binding of each can be either simultaneous or independent of the other (Shahied, 2004)....

  10. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...successfully used in conditions that are the most similar to yours. Take a look at the publications cited...application, a publication successfully used it in a similar way as you plan to. To secondary or not to secondary...

  11. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...conjugated in the lab. In the indirect approach, you can simply keep a stock of conjugated secondary antibodies...regardless of the number of protein targets, simplifying multiplex assays.  A brief note Since the indirect...

  12. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ...Fandino, L. B., Labasky, M. E., Forostyan, T., Colosimo, L. K., Graham, T. E. (2015). Rapid parallel measurements...Fluorescent Protein Oligomerization Seeing Red: Simple GFP Photoconversion Light Sheet Fluorescence Microscopy...

  13. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...With these improvements, labeling efficiency was similar to that of a comparable FISH method. Importantly...By labeling two genes of a given chromosome simultaneously, they could also monitor the spatial relationship...

  14. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    Published
    April 20, 2023, 4 p.m.
    ...getting too nervous or having a memory lapse, I simply pause and take a deep breath before the panic sets...make a strategy that can help. Even something as simple as telling yourself, “I can stop and check my notes...

  15. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...been clinically exploited for antiviral purposes similar to that of vaccines. If you know the sequence of...responses can mount in the absence of infection. Similarly, if endogenously synthesized dsRNA finds its way...

  16. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...to study and engineer them, but what if we had a simple tool that allowed us to rapidly decode the complexity...experiments. CombiGEM-CRISPR: The marriage of two simple platforms CRISPR has revolutionized how we decode...

  17. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...protein fusions, and multiple cloning sites. In the simplest case we have a standard backbone with one insert...reactions in parallel. We have a few tricks to simplify the process. First, nearly all of our Sanger sequencing...

  18. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...actually really research oriented [and] somewhat similar to an academic research environment” with a strong...multiple replicate populations, they found that similar barcodes consistently made up the majority of the...

  19. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...task, action, and results. While this method seems simple, I found it hard to remember to describe all four...answers rings true for you, go ahead and give a similar answer in a following interview. Expect to be tired...
Showing: 241 - 260 of 577 results