We narrowed to 587 results for: SIM

...strong collaborative research team motivated by similar visions and goals? We continue our conversation...and a friend. George Church runs his lab fairly similarly to his advisor Wally Gilbert: “Very interdisciplinary...

...can either express the plasmid DNA transiently (similar to bacteria) or incorporate the genetic material.... Positive selection in mammalian cells works similarly to that in bacteria and a table of the most commonly...

...management, and how does it relate to science? In the simplest of terms, a software product manager helps ensure...could code the best operations software possible. Similarly, when the developers needed to know information...

...not robust. Sampling noise There is, of course, simple noise in the process of sampling and inference-...are known. The author submits the research plan (similar to a preregistration, but usually with a fully ...

...research purposes requires stable host cells (the simplest example would be a transformed mesophilic, for... warm weather. Psychrophilic enzymes face very similar issues when forced to express in mesophilic host...

..., where multiple fluorescent tools are used simultaneously to monitor or control different cell types ...4.0 International license. Figure 5: A) Simultaneous fluorescence (upper trace) and electrophysiological...

...Packaging using 293T cells This method is very similar to lentiviral packaging methods. The three components...constructs. Why do many γ-retroviral plasmids have similar names? The nomenclature of the plasmid backbone...

... an advanced search option. For the former, you simply type in identifier keywords (Cas name, RefSeq ID...phylogenetic nomenclature and discover orthologs with similar properties (Makarova et al). CasPEDIA is an evolving...

...organisms. This is often cDNA, shRNA, or something similar, and is contained on the transfer plasmid. If expression...different from gamma-retrovirus, but they perform very similar functions to infiltrate the host cell and deposit...

...NG (Nishimasu et al., 2018). The David Liu Lab similarly described xCas9, which displayed the ability to... (HypaCas9). This nuclease showed decreased or similar off-target activity, relative to eSpCas9 and SpCas9...

...this post in the graphic below - including some simple comparisons. The easy multiplexing afforded by...integrated RNA and CRISPR/Cas toolkit in human cells. Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK. ...

...and mentees. Because the participants are at a similar development stage, they have a lot to learn from...Sometimes it is helpful to find peers that share a similar direction or problem. For example, a group of people...

...experiences. If you’re hearing multiple people with similar misconceptions, it’s time to rework your strategy...group was just really tired and needed a slower, simpler presentation than I had planned; other times students...

...variables (scFvs) are ~27kD engineered fusion proteins similar to a Fab fragment but created through engineering...different antigen; binding of each can be either simultaneous or independent of the other (Shahied, 2004)....

...successfully used in conditions that are the most similar to yours. Take a look at the publications cited...application, a publication successfully used it in a similar way as you plan to. To secondary or not to secondary...

...conjugated in the lab. In the indirect approach, you can simply keep a stock of conjugated secondary antibodies...regardless of the number of protein targets, simplifying multiplex assays.  A brief note Since the indirect...

...Assembly, Golden Gate etc.), and try to find the simplest way to do it (i.e. avoid assembling too many fragments...help you at this stage and that can be used to simulate in silico cloning. This will definitely help in...

...getting too nervous or having a memory lapse, I simply pause and take a deep breath before the panic sets...make a strategy that can help. Even something as simple as telling yourself, “I can stop and check my notes...

...Fandino, L. B., Labasky, M. E., Forostyan, T., Colosimo, L. K., Graham, T. E. (2015). Rapid parallel measurements...Fluorescent Protein Oligomerization Seeing Red: Simple GFP Photoconversion Light Sheet Fluorescence Microscopy...

...With these improvements, labeling efficiency was similar to that of a comparable FISH method. Importantly...By labeling two genes of a given chromosome simultaneously, they could also monitor the spatial relationship...