Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria
Type
Blog Post
Published
April 4, 2019, 12:53 p.m.
... it has been challenging to introduce CRISPRi machinery into diverse bacteria species. Existing protocols... disease-associated.
Mobile-CRISPRi: a modular approach for CRISPRi transfer
Mobile-CRISPRi offers two...bacteria with a chromosomal copy of the RP4 transfer machinery mobilize these plasmids for transfer to recipient...contain a chromosomal copy of the RP4 transfer machinery, which mobilizes the Tn7 plasmids, and lack the...wild-type bacteria and the relative frequency of each of the 40 library strains was measured after 6 and...