We narrowed to 284 results for: cki

1. Deciphering the Mysteries of Behavior with Viral Vectors

   Type

   Blog Post

   Published

   Sept. 17, 2019, 12:40 p.m.

   ... circular corridor apparatus using a camera or tracking software. Locomotion is a highly complex process...

2. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

   Type

   Blog Post

   Published

   Aug. 13, 2019, 12:58 p.m.

   ... target mRNA inhibit its translation either by blocking initiation or splicing. (RNAi, in contrast, engages...

3. Save Time with Transient Plant Leaf Transformations

   Type

   Blog Post

   Published

   July 25, 2019, 12:03 p.m.

   ... your tissue. Run a control with Agrobacterium lacking the GUS gene since plants can have endogenous GUS-like...

4. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

   Type

   Blog Post

   Published

   July 23, 2019, 12:46 p.m.

   ...galactose and the AAV receptor (AAVR). Cell lines lacking galactose were still transduced by PHP.B but only...

5. Plasmids 101: Codon usage bias

   Type

   Blog Post

   Published

   Sept. 27, 2018, 1:09 p.m.

   ... to balance all of these factors on their own. Luckily, many researchers have created codon optimization...

6. Twenty Years of Sharing: Expanding Accessibility

   Type

   Blog Post

   Published

   Nov. 21, 2024, 10:47 p.m.

   ...stays in transit for a certain number of days and tracking average transit time for each courier we contracted...

7. Transferable Skills Guide: External Collaborations

   Type

   Blog Post

   Published

   April 19, 2018, 1:25 p.m.

   ...studies in regard to reaching out to new people, checking in regularly, and maintaining useful notes that...

8. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

   Type

   Blog Post

   Published

   Oct. 19, 2017, 1:54 p.m.

   ...proteins needed for later experiments (cellular tracking for instance) can be controlled with FLP-FRT. ...

9. CRISPR 101: Targeting RNA with Cas13a (C2c2)

   Type

   Blog Post

   Published

   Sept. 21, 2017, 2:07 p.m.

   ...have utilized dCas13 fusion proteins for imaging, tracking, modulating splicing, and regulating expression...

10. Fluorescent Proteins 101: Aptamer Fluorophores

    Type

    Blog Post

    Published

    April 11, 2017, 2:30 p.m.

    ...fluorogen and increase its fluorescence, essentially mimicking GFP.  Though the aptamer, called Spinach, required...

11. Antibiotic Resistance: An Old Solution but a New Problem

    Type

    Blog Post

    Published

    Nov. 10, 2016, 3:30 p.m.

    ...that only infect bacterial cells), or a bacterium picking up resistance genes from it’s environment (from...

12. 5 Reasons to Use Reddit for Science Communication

    Type

    Blog Post

    Published

    Oct. 20, 2016, 2:30 p.m.

    ...off, usually via the internet, using dialogue). Luckily r/Science has a great team of moderators who facilitated...

13. CRISPR Activation: A Practical Guide

    Type

    Blog Post

    Published

    Aug. 18, 2016, 2:30 p.m.

    ... scorer 1.0 (Now 2.0) (Chari et al., 2015) and picking whichever guides are closest to the transcription...

14. Getting the Most from Your Lentiviral Transduction

    Type

    Blog Post

    Published

    April 7, 2016, 2:30 p.m.

    ...lentivirus to be time consuming, difficult, and lacking in reproducibility. Read on for some tips for getting...

15. Plasmids 101: Control Plasmids

    Type

    Blog Post

    Published

    April 9, 2015, 3:29 p.m.

    ...results can be compared; results from an experiment lacking the proper controls are invariably inconclusive...

16. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type

    Blog Post

    Published

    Aug. 5, 2014, 6:51 p.m.

    ...Instead of providing a “dead end” figure or table, clicking on the published chart could reveal the underlying...

17. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type

    Blog Post

    Published

    May 5, 2020, 1:15 p.m.

    ... so, the WHO recommends one strategy: testing, tracking, and social distancing. With countries like South...

18. The Fluorescent Vegetables in Aptamer Soup

    Type

    Blog Post

    Published

    Jan. 29, 2019, 2:27 p.m.

    ...bright, high-affinity complex for RNA labeling and tracking." ACS chemical biology 9.10 (2014): 2412-2420....

19. Antibodies 101: Introduction to Immunofluorescence

    Type

    Blog Post

    Published

    Nov. 16, 2021, 2:15 p.m.

    ...permeabilization, antigen retrieval, and sectioning. Blocking Block is used to reduce non-specific binding...

20. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type

    Blog Post

    Published

    June 3, 2021, 1:15 p.m.

    ... signal is boosted and enables single molecule tracking in living cells. The HA frankenbody is an HA tag...