We narrowed to 513 results for: gats

...specific protein by combining it with the proximity ligation assay (PLA). The clever combination of FUNCAT ...endogenous protein complexes in situ by proximity ligation." Nature methods 3.12 (2006): 995-1000. PubMed...

...problems. Why didn’t my PCR work? Why didn’t my ligation work? Why are there so many bands on my Western...process.  Step One: Identify the problem and gather information to help you understand it. Step Two...

...His work focuses on the enzyme hydropersulfide-propagating enzymes 3-mercaptopyruvate sulfurtransferase ...withdrawal to explore how these cells may encode the negative affect associated with drug withdrawal.  “Together...

... use an input signal that both positively and negatively controls the output. In this case, plasmid copy...increases its expression. However, if you introduce negative regulation by using a promoter that is responsive...

...several years, it seems that the more polarized (negative) the action potential of a cell, the more differentiated...Brook T., et al. "Use of genetically encoded, light-gated ion translocators to control tumorigenesis." Oncotarget...

...large number of targets, and can require specific conjugates. And, of course, once you have them, you’ll need...antibodies, along with a higher likelihood of aggregation (Bates, 2019). They are small enough to be produced...

...Later this week, postdocs and students will again gather in Boston - this time with the aim of finding solutions...programs? What would be the effects, positive and negative, of changing the workforce structure? Helping...

...spacer of each loxP site and the two strands are ligated, resulting in the reciprocal crossover event. This...sequence. Among these are loxN (GtATACcT), lox2272 (GgATACtT), and lox511 (GtATACAT). These variant lox sites...

...acquisition, storage, and utilization - and on self-propagation by reproduction. Both physiological processes...controlled by deliberate and compulsory actions instigated by the central nervous system signaling to peripheral...

...some scientists argue that these issues can be mitigated by using low doses and running appropriate controls...pore domain (IPD) of a channel, the PSAM acts as a gating mechanism to control ion flux. The system allows...

...and selectable markers that allow students to propagate the plasmids in either E. coli (ori and ampR) ...and instructor resources is available at our Investigations in Molecular Cell Biology Website. Many thanks...

...ball-and-chain that they resemble, actually impede your investigation into the regulation of your favorite gene. But...disease: cancer (Feinberg & Vogelstein 1983). The floodgates, as they say, had opened.  In 2021,  with over...

... culture supernatant and can be purified by centrifugation. Gag-Cas9 fusion protein The MLV Gag protein...which is both technically challenging and can negatively impact zygote viability. Nanoblades, however,...

...where and when certain genes are expressed to investigate their role in a specific behavior. This blog ...preceding the experiment. Handling can however negatively interfere with certain tests and is not always...

...These results are normally one figure and can be negative and/or even replicated studies to show reproducible...naturally “associate, self-excise, and covalently ligate their flanking peptides together (Wang et al., ...

...ribonucleoprotein (RNP) was limited (likely due to its long, negatively charged sgRNA), RNP delivery was much better ...This new capsid will be a valuable tool for investigating NHP brains. Figure 3: Overview of library...

...transformed into a bacterial cell. Transformed cells propagate, are induced to produce your protein of interest...of protein in bacterial cells: the pET, pRSET, Gateway pDEST, and pBAD vectors for example. Protein expression...

...elaborate if your audience seems captivated. Expertly navigate questions Answering questions is arguably the ..., so let us know your own ways to successfully navigate poster sessions with ease. Many thanks to our...

...So you’ve done the research, gathered up your data into an exciting story, and are ready to present your... information to prepare for the next meeting. Navigating talks Before the conference Is the conference...

...of the run, each droplet is read as positive or negative for each reaction, math happens (this time, Poisson...accurately, and raw data should be checked to confirm negative samples and, if unexpected, verified by running...