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We narrowed to 415 results for: genome

Showing: 241 - 260 of 415 results

  1. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    Published
    July 20, 2017, 2:30 p.m.
    ...these patients suffer any less acutely.  The Rare Genomics Institute works to help those who suffer from ...disease cures. Through their BeHEARD Award, the Rare Genomics Institute partners with companies to provide technological...

  2. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...bacteria that can incorporate foreign DNA into plant genomes. Eukaryotes are a bit more complicated. Yeast vectors...the plasmid is incorporated into the host cell’s genome, so no special sequence is needed to retain the...remains episomal, meaning it stays separate from the genome. One option for episomal vectors is to include ...

  3. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...dCas-VPR repressor are introduced into the hPSC genome using AAV1 and contain an EGFP reporter. Expression...The multi-gRNA piggyBac vector integrates into genomic DNA with the help of the piggyBa transposase. Image... and AAV donor infection (CRISPR-READI) enables genome engineering using HDR donors lengths up to 4.9 ...

  4. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...proteins and genomic DNA to precipitate. Plasmid DNA is free in solution. Proteins and genomic DNA are removed... on the type of DNA you’re purifying (plasmid, genomic, or DNA fragments from agarose).    In this article...

  5. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...considered an authority in this area. What is genome engineering? How does CRISPR work? This section...that, like computers and the internet before it, genome engineering technology is moving along much faster...examples of how understanding genetics is so timely.” Genome engineering, and many other technologies and fields...

  6. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ... Dotson.  Resources and references References Genome-scale CRISPR-Cas9 knockout and transcriptional ...screening, and validation of CRISPR libraries. The Genome Engineering using CRISPR/Cas Systems Forum- Moderated... OO, Platt RJ, Brigham MD, Sanjana NE, Zhang F. Genome-scale CRISPR-Cas9 knockout and transcriptional ...

  7. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...events likely due to insertional effects in the genome. (Bottom panel, right) A highly expressing RUBY...Addgene blog  CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9 Tips for Arabidopsis Transformation...

  8. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    Published
    May 2, 2023, 1 p.m.
    ...to introduce reporters throughout an organism’s genome. These reporters have historically been used extensively...Examples of promoter, enhancer, and gene traps at a genomic locus.    Choosing a trap At first glance...

  9. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...p-element, random or targeted, integration into the fly genome and are under the Gal4 inducible UAS promoter. ...From making transgenic animals, gene targeting and genome modifications to reporter vectors (e.g. pBV-Luc...

  11. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...efficiency using XMAS-TREE-based methods at various genomic loci in four different categories.     Brookhouser...resistance.  The MYC-CRISPR library targets E-boxes genome-wide. It was designed based on MYC-ChIP-seq data...Cas13Y, two compact Cas13 proteins found in the metagenome datasets from hypersaline samples, were engineered...

  12. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    Published
    Sept. 14, 2021, 1:15 p.m.
    ...carries the transgene of interest (aka the viral genome) and the other contains the bacterial selection...paired-gRNA plasmid cloning strategy for efficient genome editing in Escherichia coli. Microb Cell Fact 19...lentiviral expression vectors using reduced-genomeEscherichia coli. BioTechniques 43:466–470. https://doi.org...

  13. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ... amplifying from plasmid DNA (rather than from genomic DNA or a cDNA library), roughly 18-21bp is usually...increase the Tm if you are trying to clone from genomic DNA, a cDNA library, or by RT-PCR. Isolate the ...

  14. CRISPR Guide Update

    Type
    Blog Post
    Published
    Oct. 10, 2024, 1:15 p.m.
    ...additions describing CRISPR’s ability to make large genome edits using transposases or the Cascade-Cas3 system...

  15. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

    Type
    Blog Post
    Published
    Sept. 9, 2025, 1:15 p.m.
    ...genetic tools, enhancers were nominated from the genome based on their differential chromatin accessibility...were derived from the human, mouse, and macaque genomes, and are designated by a letter following the enhancer... of enhancer action: the known and the unknown. Genome Biology, 22(1). https://doi.org/10.1186/s13059-...

  16. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ... a nuclease-deficient Cas9 can bind loci in the genome that are defined by a gRNA. Since Cas9 is a protein...protein, a fusion with GFP allows for detection of genomic locations (Chen et al., 2013). GFP fluorescence.... 13. Chen, Baohui, et al. "Dynamic imaging of genomic loci in living human cells by an optimized CRISPR...

  17. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...concentrations in cells than existing sensors. Using genome mining and structure-guided engineering of potassium...engineering. Figure 5: Structure-guided design and genome mining both led to new sensors with lower binding...Potassium Indicators through Structure-Guided and Genome Mining Strategies. ACS Sens. 2022 May 27;7(5):1336...

  18. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...guide RNAs (gRNAs) on a single array to target the genome of the invading phage pathogen. Over the past decade...This could also enable more convenient multiplex genome engineering of crops or allow for sophisticated...

  20. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...library The transcription factor library The human genome encodes approximately 1,800 TFs, with >3,500 unique...doi: 10.1016/j.cell.2022.11.026 Joung J. et al., Genome-scale CRISPR Cas-9 knockout and transcriptional...
Showing: 241 - 260 of 415 results