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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 411 results for: genome

Showing: 241 - 260 of 411 results

  1. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ... Dotson.  Resources and references References Genome-scale CRISPR-Cas9 knockout and transcriptional ...screening, and validation of CRISPR libraries. The Genome Engineering using CRISPR/Cas Systems Forum- Moderated... OO, Platt RJ, Brigham MD, Sanjana NE, Zhang F. Genome-scale CRISPR-Cas9 knockout and transcriptional ...

  2. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...dCas-VPR repressor are introduced into the hPSC genome using AAV1 and contain an EGFP reporter. Expression...The multi-gRNA piggyBac vector integrates into genomic DNA with the help of the piggyBa transposase. Image... and AAV donor infection (CRISPR-READI) enables genome engineering using HDR donors lengths up to 4.9 ...

  3. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...proteins and genomic DNA to precipitate. Plasmid DNA is free in solution. Proteins and genomic DNA are removed... on the type of DNA you’re purifying (plasmid, genomic, or DNA fragments from agarose).    In this article...

  4. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...considered an authority in this area. What is genome engineering? How does CRISPR work? This section...that, like computers and the internet before it, genome engineering technology is moving along much faster...examples of how understanding genetics is so timely.” Genome engineering, and many other technologies and fields...

  5. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    Published
    May 2, 2023, 1 p.m.
    ...to introduce reporters throughout an organism’s genome. These reporters have historically been used extensively...Examples of promoter, enhancer, and gene traps at a genomic locus.    Choosing a trap At first glance...

  6. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...events likely due to insertional effects in the genome. (Bottom panel, right) A highly expressing RUBY...Addgene blog  CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9 Tips for Arabidopsis Transformation...

  7. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...p-element, random or targeted, integration into the fly genome and are under the Gal4 inducible UAS promoter. ...From making transgenic animals, gene targeting and genome modifications to reporter vectors (e.g. pBV-Luc...

  8. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...efficiency using XMAS-TREE-based methods at various genomic loci in four different categories.     Brookhouser...resistance.  The MYC-CRISPR library targets E-boxes genome-wide. It was designed based on MYC-ChIP-seq data...Cas13Y, two compact Cas13 proteins found in the metagenome datasets from hypersaline samples, were engineered...

  9. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    Published
    Sept. 14, 2021, 1:15 p.m.
    ...carries the transgene of interest (aka the viral genome) and the other contains the bacterial selection...paired-gRNA plasmid cloning strategy for efficient genome editing in Escherichia coli. Microb Cell Fact 19...lentiviral expression vectors using reduced-genomeEscherichia coli. BioTechniques 43:466–470. https://doi.org...

  11. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ... amplifying from plasmid DNA (rather than from genomic DNA or a cDNA library), roughly 18-21bp is usually...increase the Tm if you are trying to clone from genomic DNA, a cDNA library, or by RT-PCR. Isolate the ...

  12. CRISPR Guide Update

    Type
    Blog Post
    Published
    Oct. 10, 2024, 1:15 p.m.
    ...additions describing CRISPR’s ability to make large genome edits using transposases or the Cascade-Cas3 system...

  13. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...concentrations in cells than existing sensors. Using genome mining and structure-guided engineering of potassium...engineering. Figure 5: Structure-guided design and genome mining both led to new sensors with lower binding...Potassium Indicators through Structure-Guided and Genome Mining Strategies. ACS Sens. 2022 May 27;7(5):1336...

  14. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ... a nuclease-deficient Cas9 can bind loci in the genome that are defined by a gRNA. Since Cas9 is a protein...protein, a fusion with GFP allows for detection of genomic locations (Chen et al., 2013). GFP fluorescence.... 13. Chen, Baohui, et al. "Dynamic imaging of genomic loci in living human cells by an optimized CRISPR...

  15. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...library The transcription factor library The human genome encodes approximately 1,800 TFs, with >3,500 unique...doi: 10.1016/j.cell.2022.11.026 Joung J. et al., Genome-scale CRISPR Cas-9 knockout and transcriptional...

  16. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ..., green chemistry and understanding the minimal genome. Many of these techniques require more complex ...Assembly® is a registered trademark of Synthetic Genomics, Inc. One or more of these products are covered...

  18. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...guide RNAs (gRNAs) on a single array to target the genome of the invading phage pathogen. Over the past decade...This could also enable more convenient multiplex genome engineering of crops or allow for sophisticated...

  19. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ... cell lines from becoming the new HeLa cells?), genome engineering and stem cells, and the difficulty ...progenitors into a pig. In stark contrast to recent genome engineering technologies, stem cell techniques ...

  20. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...is causal for a given disease. CRISPR has made genomic SDM relatively straightforward, but plasmid-based...separate plasmid or be inserted into the bacterial genome. REPLACR-mutagenesis starts out in familiar territory...
Showing: 241 - 260 of 411 results